FastQCFastQC Report
Thu 26 May 2016
SRR1547723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547723_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1344113
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133610.9940384476602785No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18210.13547968065184995No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17960.1336197179850206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAG453.8562575E-1045.01
CCGCTAT207.033715E-445.011
TCTTGCG502.1827873E-1144.9999961
CGACGGT5500.044.59090828
CGTTTTT78850.043.2308161
GCGCGTC1050.042.85714338
CGCATCG900.042.521
GGACCGT750.042.0000048
CTCACGA6000.041.62500424
ACGACGG5900.041.567827
AATGCGG1250.041.3999982
ATACGCG603.6379788E-1241.2499961
CACGACG5950.041.21848726
TCACGAC6050.040.90909225
TCCGTGC556.184564E-1140.90909233
CTAACCT6150.040.243915
TCTCACG6250.039.96000323
GACGGTC6200.039.55645429
CGGTCTA6150.039.51219631
CTAGACG403.4588084E-739.3751
Produced by FastQC (version 0.11.5)