FastQCFastQC Report
Thu 26 May 2016
SRR1547722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547722_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1330809
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68290.51314651463884No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27900.20964691401996832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA207.033688E-445.00000427
CAAGTCG207.033688E-445.0000041
ACGTTGT207.033688E-445.00000437
GCGGTAG207.033688E-445.00000441
CTAAGCG207.033688E-445.0000041
TCGATCT207.033688E-445.00000435
ATCCGGC207.033688E-445.00000418
GCCGACC207.033688E-445.00000414
CGTAGCA207.033688E-445.00000437
ACAATCG207.033688E-445.00000444
TTACGCT207.033688E-445.00000442
TAATGCG406.8157533E-945.0000041
TAGTCGT207.033688E-445.00000440
TAGTCGA207.033688E-445.00000431
ATCGATC207.033688E-445.00000434
AGTCGTA207.033688E-445.00000441
CGGACGT207.033688E-445.00000418
GTCGACG351.21212E-745.01
TTAGACG253.8911392E-545.01
GAATACG253.8911392E-545.01