##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547722_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1330809
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.705756423348504	31.0	31.0	33.0	30.0	34.0
2	32.08257533575442	33.0	31.0	34.0	30.0	34.0
3	32.12901250292116	33.0	31.0	34.0	30.0	34.0
4	35.828089530503625	37.0	35.0	37.0	35.0	37.0
5	35.752670743885865	37.0	35.0	37.0	35.0	37.0
6	35.80925587368285	37.0	35.0	37.0	35.0	37.0
7	36.16586602585345	37.0	35.0	37.0	35.0	37.0
8	36.07583732902317	37.0	35.0	37.0	35.0	37.0
9	37.93359302499457	39.0	39.0	39.0	35.0	39.0
10	37.55715433243989	39.0	37.0	39.0	35.0	39.0
11	37.14052204335859	39.0	37.0	39.0	34.0	39.0
12	36.271040397232056	38.0	35.0	39.0	33.0	39.0
13	35.93831496480712	38.0	35.0	39.0	32.0	39.0
14	36.85026927229978	39.0	35.0	41.0	32.0	41.0
15	37.12053645564465	39.0	35.0	41.0	33.0	41.0
16	37.28454421333189	39.0	35.0	41.0	33.0	41.0
17	37.20976413595039	38.0	35.0	41.0	33.0	41.0
18	37.13069343534647	38.0	35.0	41.0	33.0	41.0
19	37.09758274853867	38.0	35.0	41.0	33.0	41.0
20	36.988567856093546	38.0	35.0	41.0	33.0	41.0
21	36.83300608877758	38.0	35.0	41.0	32.0	41.0
22	36.76657581967059	38.0	35.0	41.0	32.0	41.0
23	36.70690835424167	38.0	35.0	41.0	32.0	41.0
24	36.71416108547508	38.0	35.0	41.0	32.0	41.0
25	36.64745203857203	38.0	35.0	40.0	32.0	41.0
26	36.60458638316994	38.0	35.0	40.0	32.0	41.0
27	36.53054570565723	38.0	35.0	40.0	32.0	41.0
28	36.548927006054214	38.0	35.0	40.0	32.0	41.0
29	36.53799831531046	38.0	35.0	40.0	32.0	41.0
30	36.56608423898546	38.0	35.0	40.0	32.0	41.0
31	36.40664287662617	38.0	35.0	40.0	31.0	41.0
32	36.16646941822606	38.0	35.0	40.0	31.0	41.0
33	36.07280909582066	38.0	35.0	40.0	31.0	41.0
34	35.90086781799642	38.0	35.0	40.0	30.0	41.0
35	35.795019420517896	38.0	35.0	40.0	30.0	41.0
36	35.705053843188615	38.0	35.0	40.0	30.0	41.0
37	35.47249304746211	37.0	35.0	40.0	29.0	41.0
38	35.598805688870456	37.0	35.0	40.0	30.0	41.0
39	35.552812612478576	37.0	35.0	40.0	30.0	41.0
40	35.452671269881705	37.0	35.0	40.0	29.0	41.0
41	35.427158217294895	37.0	35.0	40.0	29.0	41.0
42	35.42383016646266	37.0	35.0	40.0	29.0	41.0
43	35.31266395102528	37.0	34.0	40.0	29.0	41.0
44	35.20256700999167	36.0	34.0	40.0	29.0	41.0
45	35.18686227700594	36.0	34.0	40.0	28.0	41.0
46	35.021852121529086	36.0	34.0	40.0	28.0	41.0
47	34.898521876542766	36.0	34.0	40.0	27.0	41.0
48	34.78619847025381	36.0	34.0	40.0	27.0	41.0
49	34.88532013234055	36.0	34.0	40.0	27.0	41.0
50	34.75707708619343	36.0	34.0	40.0	27.0	41.0
51	34.14467290197166	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	4.0
14	12.0
15	36.0
16	89.0
17	175.0
18	384.0
19	767.0
20	1390.0
21	2426.0
22	3739.0
23	5596.0
24	8410.0
25	12628.0
26	17249.0
27	19841.0
28	21372.0
29	23514.0
30	27857.0
31	34590.0
32	44516.0
33	61043.0
34	119899.0
35	227920.0
36	77219.0
37	100395.0
38	167220.0
39	352419.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.191252839438267	23.7898902096394	30.731607616119216	14.287249334803114
2	30.352890610147664	25.311370752677508	31.998431029546687	12.337307607628142
3	27.84847412363457	25.001784628748382	33.33626388159382	13.813477366023225
4	26.285364766844825	27.268601279372174	32.466417044068685	13.979616909714315
5	23.507580727211792	31.730098008053748	31.3498781568204	13.412443107914058
6	21.823266900058535	40.52174278953629	27.026267480908228	10.628722829496946
7	88.53486864005278	3.589621050053013	6.521972724861344	1.3535375850328635
8	90.04432642099655	2.882156643064482	5.809774355298168	1.263742580640798
9	87.78389686273538	3.7792049798280596	6.486205007630696	1.95069314980587
10	54.83927445636452	26.807453210791333	10.743164496182397	7.61010783666176
11	42.81681293108177	20.334773810516758	22.628341106800452	14.220072151601018
12	39.734928152725146	20.84281065126551	24.93956683491019	14.482694361099153
13	23.7544982037242	36.96834031029246	24.565658933776373	14.711502552206964
14	15.20894433386008	41.70425658377724	27.244480612920412	15.84231846944227
15	13.670406497100634	26.58172585247019	45.636676638044975	14.111191012384197
16	18.20952518355376	21.29095910833185	44.83844037724422	15.661075330870169
17	18.594028143783216	21.029163463727702	28.911586861826155	31.46522153066293
18	21.65427195037004	25.207599287350778	34.275767597003025	18.86236116527616
19	27.0257414850666	26.162131455377896	27.312108649701045	19.50001840985446
20	30.317949457811	25.25298521425689	26.95976657807394	17.46929874985817
21	23.43769842253847	27.178505705927748	30.537740577348067	18.846055294185717
22	23.09888195826749	22.49909641428635	29.693517251536473	24.70850437590969
23	17.92488629097038	28.593735088957168	29.700054628425264	23.781323991647184
24	19.735589404640336	25.147786045931458	38.13665221680947	16.979972332618733
25	17.720499335366682	26.27198944401488	36.020420661417226	19.987090559201206
26	16.5483551734321	36.15289647124418	27.04678131873169	20.251967036592028
27	17.49529797288717	34.60000646223463	31.44786366788923	16.456831896988973
28	14.812268327010111	28.89603241336661	39.25484423384572	17.03685502577755
29	15.115317074050447	25.849689925451365	39.11560562034071	19.91938738015748
30	18.03962852670819	30.492504934968128	33.90877278407345	17.559093754250235
31	28.62469370135008	27.28400544330554	26.59667916282502	17.494621692519363
32	28.13724584068788	27.456757506148517	28.510853172769345	15.895143480394255
33	27.20863775342667	28.846062808412025	26.188581531985434	17.75671790617587
34	20.610095062477036	28.492518460575482	28.179100081228786	22.718286395718696
35	18.820807493787616	27.681733441838762	31.12813333844301	22.36932572593062
36	28.19397824932053	24.661540461478694	29.733643220026313	17.410838069174464
37	20.11603468266295	30.84634985185703	31.099654420732055	17.93796104474797
38	19.6773541507459	32.483925191368556	25.372611697095525	22.466108960790017
39	19.4347197832296	30.744982938949168	29.94096072389051	19.87933655393073
40	23.23083177225282	27.35569116229301	26.68910414642522	22.72437291902895
41	17.90076562451862	24.703394702019597	30.288568832942968	27.10727084051881
42	22.314096162559764	24.954970998843564	27.215099988052383	25.515832850544296
43	22.231063961845766	25.65942971530851	27.301513590605413	24.807992732240315
44	19.34176880378777	30.4804070306107	29.260848100666585	20.91697606493494
45	17.83396415263197	36.12486840711176	25.26989222345205	20.771275216804213
46	23.504950748003658	32.09596568703698	26.458793110055613	17.940290454903746
47	21.147061674515278	29.527603134634646	27.138680306490265	22.186654884359815
48	22.414636510573644	26.6300423276368	30.961618083436466	19.993703078353093
49	21.303057012689276	23.720834469860065	32.66479261862521	22.311315898825452
50	20.053140608456964	31.536756965124223	28.62649711566423	19.783605310754588
51	18.685551420226343	33.30598154956872	26.91122467611806	21.097242354086877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1301.0
1	1677.0
2	2053.0
3	6636.5
4	11220.0
5	8033.5
6	4847.0
7	5150.0
8	5453.0
9	5905.0
10	6357.0
11	6451.5
12	6546.0
13	6337.5
14	6129.0
15	5924.5
16	5720.0
17	5466.5
18	5213.0
19	4746.0
20	4279.0
21	4690.5
22	5102.0
23	5518.0
24	5934.0
25	7259.5
26	10780.0
27	12975.0
28	15107.5
29	17240.0
30	19248.5
31	21257.0
32	26961.5
33	32666.0
34	37011.0
35	41356.0
36	44013.5
37	46671.0
38	52876.0
39	59081.0
40	82227.5
41	105374.0
42	121594.0
43	137814.0
44	141831.0
45	145848.0
46	136434.5
47	127021.0
48	120030.0
49	113039.0
50	102328.5
51	91618.0
52	82336.5
53	73055.0
54	67180.0
55	61305.0
56	56580.5
57	51856.0
58	46320.0
59	40784.0
60	34511.5
61	28239.0
62	23698.5
63	19158.0
64	15567.5
65	11977.0
66	9198.0
67	6419.0
68	5004.0
69	3589.0
70	2622.5
71	1656.0
72	1349.5
73	1043.0
74	839.5
75	425.5
76	215.0
77	124.5
78	34.0
79	61.0
80	88.0
81	55.5
82	23.0
83	16.5
84	10.0
85	8.5
86	7.0
87	5.5
88	4.0
89	3.0
90	2.0
91	3.5
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1330809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.01777836819444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.9515097828432	14.765841402061872
2	6.66866074745152	2.4030890272051875
3	2.005502960367493	1.0840412356997797
4	0.9336417954467968	0.6728860378257405
5	0.5943525512692367	0.5354456270670015
6	0.4246023824935359	0.45902349734255116
7	0.314173269793766	0.3962493041048523
8	0.26799709292538626	0.3862969778920015
9	0.23793897542624315	0.3858418551946781
>10	3.181868091689674	14.594002441832727
>50	1.9218823855864622	25.64234721601229
>100	1.492423164835039	37.3764778347305
>500	0.004608830660635999	0.5723027112625997
>1k	4.1898460551236357E-4	0.21062189215441934
>5k	4.1898460551236357E-4	0.5155329396138099
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6829	0.51314651463884	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2790	0.20964691401996832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.514226308959437E-5	0.0	0.0	0.029305482604941806	0.0
2	7.514226308959437E-5	0.0	0.0	0.1376606259801369	0.0
3	7.514226308959437E-5	0.0	0.0	0.19198848219391362	0.0
4	7.514226308959437E-5	0.0	0.0	0.2809569216919934	0.0
5	7.514226308959437E-5	0.0	0.0	0.49638978996986044	0.0
6	7.514226308959437E-5	0.0	0.0	0.6511077096713352	0.0
7	7.514226308959437E-5	0.0	0.0	0.7656245186198771	0.0
8	7.514226308959437E-5	0.0	0.0	0.9531796072915046	0.0
9	7.514226308959437E-5	0.0	0.0	1.0214839244399458	0.0
10	7.514226308959437E-5	0.0	0.0	1.1951376944399985	0.0
11	7.514226308959437E-5	0.0	0.0	1.4413037483215096	0.0
12	7.514226308959437E-5	0.0	0.0	1.6419335907707266	0.0
13	7.514226308959437E-5	0.0	0.0	1.716549858018694	0.0
14	7.514226308959437E-5	0.0	0.0	1.7447282066772918	0.0
15	7.514226308959437E-5	0.0	0.0	1.7968017949983808	0.0
16	7.514226308959437E-5	0.0	0.0	1.8980184233800643	0.0
17	7.514226308959437E-5	0.0	0.0	2.0248585634753	0.0
18	7.514226308959437E-5	0.0	0.0	2.1828827427527164	0.0
19	7.514226308959437E-5	0.0	0.0	2.2875559152365215	0.0
20	7.514226308959437E-5	0.0	0.0	2.3802063256259913	0.0
21	7.514226308959437E-5	0.0	0.0	2.5149364033456343	0.0
22	7.514226308959437E-5	0.0	0.0	2.6583829835836696	0.0
23	7.514226308959437E-5	0.0	0.0	2.8182857194383266	0.0
24	7.514226308959437E-5	0.0	0.0	2.932877670649958	0.0
25	7.514226308959437E-5	0.0	0.0	3.024926942934711	0.0
26	7.514226308959437E-5	0.0	0.0	3.126068429053305	0.0
27	7.514226308959437E-5	0.0	7.514226308959437E-5	3.2236782288066883	0.0
28	7.514226308959437E-5	0.0	7.514226308959437E-5	3.3254959952930885	0.0
29	7.514226308959437E-5	0.0	7.514226308959437E-5	3.438735385769107	0.0
30	7.514226308959437E-5	0.0	7.514226308959437E-5	3.573690890278019	0.0
31	7.514226308959437E-5	0.0	7.514226308959437E-5	3.698953042848373	0.0
32	7.514226308959437E-5	0.0	7.514226308959437E-5	3.8085104624330013	0.0
33	7.514226308959437E-5	0.0	7.514226308959437E-5	3.916489894492748	0.0
34	7.514226308959437E-5	0.0	7.514226308959437E-5	4.031758126072186	0.0
35	7.514226308959437E-5	0.0	7.514226308959437E-5	4.193238849451724	0.0
36	7.514226308959437E-5	0.0	7.514226308959437E-5	4.321431550282573	0.0
37	7.514226308959437E-5	0.0	7.514226308959437E-5	4.4542830714249755	0.0
38	7.514226308959437E-5	0.0	7.514226308959437E-5	4.595550526033413	0.0
39	7.514226308959437E-5	0.0	7.514226308959437E-5	4.76093864709361	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	7.033688E-4	45.000004	27
CAAGTCG	20	7.033688E-4	45.000004	1
ACGTTGT	20	7.033688E-4	45.000004	37
GCGGTAG	20	7.033688E-4	45.000004	41
CTAAGCG	20	7.033688E-4	45.000004	1
TCGATCT	20	7.033688E-4	45.000004	35
ATCCGGC	20	7.033688E-4	45.000004	18
GCCGACC	20	7.033688E-4	45.000004	14
CGTAGCA	20	7.033688E-4	45.000004	37
ACAATCG	20	7.033688E-4	45.000004	44
TTACGCT	20	7.033688E-4	45.000004	42
TAATGCG	40	6.8157533E-9	45.000004	1
TAGTCGT	20	7.033688E-4	45.000004	40
TAGTCGA	20	7.033688E-4	45.000004	31
ATCGATC	20	7.033688E-4	45.000004	34
AGTCGTA	20	7.033688E-4	45.000004	41
CGGACGT	20	7.033688E-4	45.000004	18
GTCGACG	35	1.21212E-7	45.0	1
TTAGACG	25	3.8911392E-5	45.0	1
GAATACG	25	3.8911392E-5	45.0	1
>>END_MODULE