FastQCFastQC Report
Thu 26 May 2016
SRR1547721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547721_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1233864
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64690.5242879280050313No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12460.10098357679614609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG453.8562575E-1045.01
CGTACGT207.033489E-445.029
ATGCGCG453.8562575E-1045.01
TCGAATA302.1656324E-644.99999630
CGACGGT2650.043.30188828
TTGTACG800.042.18751
CGTTTTT41750.041.8742521
CCGCTAT700.041.78571720
TCACGAC2700.041.66666425
ACGGTCT2700.041.66666430
CGTAAGG1850.041.3513532
GACGGTC2800.040.98214329
CGGTCTA2800.040.98214331
GCGTAAG1450.040.344831
TCGGTAG403.458499E-739.3751
GCGGGAT7300.039.1438375
GACAACG356.2486615E-638.571431
CAACCGG356.2486615E-638.571432
CGACCCG356.2486615E-638.5714332
GCGTTAG1050.038.571431
Produced by FastQC (version 0.11.5)