##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547721_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1233864
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.605272542192658	31.0	31.0	33.0	30.0	34.0
2	31.97673244377014	31.0	31.0	34.0	30.0	34.0
3	32.03239660124617	33.0	31.0	34.0	30.0	34.0
4	35.74784822314291	37.0	35.0	37.0	35.0	37.0
5	35.69500447375076	37.0	35.0	37.0	33.0	37.0
6	35.748594658730624	37.0	35.0	37.0	33.0	37.0
7	36.06393087082531	37.0	35.0	37.0	35.0	37.0
8	35.91987528609312	37.0	35.0	37.0	35.0	37.0
9	37.63969935098196	39.0	37.0	39.0	35.0	39.0
10	37.355957382661295	39.0	37.0	39.0	34.0	39.0
11	37.10730031834951	39.0	37.0	39.0	33.0	39.0
12	36.57282407137254	39.0	35.0	39.0	33.0	39.0
13	36.379791451894214	39.0	35.0	39.0	32.0	39.0
14	37.411568860101276	40.0	35.0	41.0	33.0	41.0
15	37.58354648486381	40.0	35.0	41.0	33.0	41.0
16	37.642109665246736	40.0	35.0	41.0	33.0	41.0
17	37.533369155757846	39.0	35.0	41.0	33.0	41.0
18	37.41039450052842	39.0	35.0	41.0	32.0	41.0
19	37.38576131567174	39.0	35.0	41.0	32.0	41.0
20	37.297509287895586	39.0	35.0	41.0	32.0	41.0
21	37.16390299092931	39.0	35.0	41.0	32.0	41.0
22	37.10426513781098	39.0	35.0	41.0	32.0	41.0
23	36.99660659521633	39.0	35.0	41.0	32.0	41.0
24	37.00727470774737	39.0	35.0	41.0	32.0	41.0
25	36.92691090752303	39.0	35.0	41.0	32.0	41.0
26	36.87289279855803	39.0	35.0	41.0	32.0	41.0
27	36.77552631408324	38.0	35.0	41.0	31.0	41.0
28	36.767625119137925	38.0	35.0	41.0	31.0	41.0
29	36.67664831780488	38.0	35.0	41.0	31.0	41.0
30	36.66198543761711	38.0	35.0	41.0	31.0	41.0
31	36.539946866105176	38.0	35.0	41.0	31.0	41.0
32	36.35114323782848	38.0	35.0	41.0	30.0	41.0
33	36.25759727166041	38.0	35.0	41.0	30.0	41.0
34	36.073754481855374	38.0	35.0	41.0	30.0	41.0
35	35.97035248617352	38.0	35.0	41.0	30.0	41.0
36	35.88846906952468	38.0	35.0	41.0	29.0	41.0
37	35.63464774075587	38.0	35.0	41.0	27.0	41.0
38	35.745282300156255	38.0	35.0	41.0	29.0	41.0
39	35.66462997542679	38.0	35.0	40.0	28.0	41.0
40	35.56695632581873	38.0	35.0	40.0	27.0	41.0
41	35.50717177906155	38.0	35.0	40.0	27.0	41.0
42	35.51495302561709	38.0	35.0	40.0	27.0	41.0
43	35.4287855063443	38.0	35.0	40.0	27.0	41.0
44	35.2934391472642	38.0	34.0	40.0	27.0	41.0
45	35.26771345950607	38.0	34.0	40.0	27.0	41.0
46	35.07769332762768	38.0	34.0	40.0	26.0	41.0
47	34.97690831404434	37.0	34.0	40.0	26.0	41.0
48	34.85434861540656	37.0	34.0	40.0	25.0	41.0
49	34.913548008532544	37.0	34.0	40.0	26.0	41.0
50	34.78692303203594	37.0	34.0	40.0	26.0	41.0
51	34.17935120888526	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	5.0
12	8.0
13	16.0
14	29.0
15	49.0
16	109.0
17	227.0
18	522.0
19	1020.0
20	1744.0
21	2865.0
22	4391.0
23	6255.0
24	8985.0
25	12696.0
26	16915.0
27	19445.0
28	20984.0
29	22717.0
30	26388.0
31	31878.0
32	39852.0
33	52399.0
34	92840.0
35	149102.0
36	79149.0
37	107388.0
38	176498.0
39	359309.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.992893868367986	22.72989567731938	26.36530444198064	13.91190601233199
2	34.305320521548566	23.954828084780818	27.46031977592344	14.279531617747173
3	30.995150194835087	24.403256760874783	30.272055915400724	14.329537128889408
4	27.099421005880714	26.205562363437135	30.231856995584604	16.463159635097547
5	24.106951819649492	31.24161171733676	28.977342721726217	15.674093741287532
6	23.180188416227395	39.342585568587786	26.688921955742284	10.788304059442531
7	83.58668378362607	5.230641302445002	8.406112829290748	2.776562084638177
8	83.23575369732806	4.9478710781739315	8.850489194919376	2.9658860295786247
9	78.24257778815169	7.192121660085713	10.397012960909793	4.168287590852801
10	42.23423327044147	31.02092288939462	16.049499782796158	10.695344057367748
11	35.179322842712004	23.548705529944954	25.775450130646487	15.496521496696555
12	32.74291169853404	22.628101638430167	28.642378738661634	15.98660792437416
13	24.050786796599947	31.603726180519086	27.32464842154403	17.020838601336937
14	17.58881043615828	34.49132157190744	30.069197253506058	17.850670738428224
15	17.12238950159823	28.057468246095198	38.95421213359009	15.865930118716488
16	21.22097735244727	24.765614362685028	37.465312222416735	16.548096062450966
17	21.692909429240174	24.4204385572478	29.010895852379193	24.87575616113283
18	22.756721972599898	26.333534327932412	32.79178256274597	18.117961136721714
19	26.40550336179676	27.527750222066615	27.45318770950445	18.613558706632176
20	27.95737617760142	27.550524206881793	27.239630947981304	17.25246866753548
21	24.833531086084044	27.418662024339795	29.67490744522897	18.07289944434719
22	23.427622493240747	23.594334545784623	30.34410599547438	22.63393696550025
23	20.645306127741794	27.694948551866332	30.31217378900754	21.347571531384336
24	21.041622091251547	25.6631200845474	34.37737060162222	18.917887222578823
25	19.791241174067807	27.693327627680198	32.55796424889615	19.957466949355844
26	19.068146894633443	32.73221359890555	28.178956513845932	20.02068299261507
27	18.564687842420234	32.182639253596825	30.817658996453417	18.43501390752952
28	17.619851134322744	29.020378258868075	34.99915711942321	18.36061348738597
29	18.64946217735504	27.012944700550467	34.682266441034024	19.655326681060473
30	20.61912820213573	28.80479534211226	32.02403182198362	18.55204463376839
31	25.545440988634077	27.578079918046072	28.155209974519074	18.721269118800777
32	26.104984017687528	27.96507556748556	28.054712674978767	17.87522773984815
33	24.905419073739083	27.882165295364803	27.812708693988963	19.399706936907148
34	20.26098500320943	28.768729778970776	29.61315023373727	21.357134984082524
35	20.246234593115613	28.83883475002107	30.51098014043687	20.403950516426445
36	24.772908521522634	28.10682538756297	28.12846472544786	18.991801365466536
37	20.80885737812271	31.484750345256852	28.602666096101352	19.103726180519086
38	20.33019846595735	31.937960747699908	26.494816284452742	21.237024501889998
39	20.026194134847923	30.803962187080586	28.403697652253406	20.76614602581808
40	23.128886165736258	27.783775197266475	27.36703558901143	21.72030304798584
41	19.19149922519824	26.97817587675789	28.58232349756537	25.248001400478497
42	21.16999928679336	27.347017175312676	27.42676664527047	24.056216892623496
43	21.582200307327227	27.003381247852275	27.610984679024593	23.803433765795905
44	19.938583182587383	29.702138971556025	29.25516912722958	21.104108718627014
45	19.85696964981554	32.82574092444548	26.070539378732178	21.246750047006802
46	21.182318310607975	31.528920529329003	27.560654983045136	19.72810617701789
47	20.60867324113517	29.766084430699006	27.652318245771006	21.97292408239482
48	21.166838484630397	27.88945945420241	30.682960196585686	20.26074186458151
49	21.304049716986636	26.743871285652226	30.558473219090597	21.393605778270537
50	19.95074011398339	30.58238185083607	29.16318167966648	20.30369635551406
51	19.206979051175818	31.81987642074005	27.143834328580784	21.82931019950335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	906.0
1	1686.5
2	2467.0
3	7327.5
4	12188.0
5	8857.0
6	5526.0
7	5576.5
8	5627.0
9	5906.0
10	6185.0
11	6123.0
12	6061.0
13	5996.0
14	5931.0
15	5542.5
16	5154.0
17	4906.0
18	4658.0
19	5113.0
20	5568.0
21	5704.5
22	5841.0
23	6758.5
24	7676.0
25	9473.5
26	12665.5
27	14060.0
28	17210.5
29	20361.0
30	22981.0
31	25601.0
32	29300.0
33	32999.0
34	38400.5
35	43802.0
36	45721.0
37	47640.0
38	55016.5
39	62393.0
40	72280.0
41	82167.0
42	92034.5
43	101902.0
44	105605.5
45	109309.0
46	105981.5
47	102654.0
48	99128.5
49	95603.0
50	89272.5
51	82942.0
52	77787.0
53	72632.0
54	66302.0
55	59972.0
56	56930.0
57	53888.0
58	48475.0
59	43062.0
60	38369.0
61	33676.0
62	28837.5
63	23999.0
64	19891.0
65	15783.0
66	12789.0
67	9795.0
68	7952.5
69	6110.0
70	4920.0
71	3730.0
72	3024.5
73	2319.0
74	1681.5
75	887.0
76	730.0
77	541.5
78	353.0
79	259.0
80	165.0
81	100.0
82	35.0
83	32.5
84	30.0
85	25.5
86	21.0
87	16.0
88	11.0
89	7.0
90	3.0
91	5.0
92	7.0
93	6.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1233864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.984160936959288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40158653838839	17.675614180467896
2	5.0967673013458885	2.2409630522203936
3	1.7508932047627295	1.154757539907968
4	1.0251177629745925	0.901454155222765
5	0.7388330866569099	0.8121312741307948
6	0.5702624109756935	0.7522044371512839
7	0.4766325550321176	0.7334856758334124
8	0.4351805453532584	0.7653663316543794
9	0.39068188730735687	0.7729932137147932
>10	6.866598254005823	38.74403483059745
>50	2.129081684074947	31.293343747796747
>100	0.11688057139039854	3.4332883064344877
>500	7.420988659707821E-4	0.09103446862555253
>1k	3.7104943298539106E-4	0.10163884221096636
>5k	3.7104943298539106E-4	0.5276899440310926
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6469	0.5242879280050313	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1246	0.10098357679614609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01823539709400712	0.0
2	0.0	0.0	0.0	0.06078465698002373	0.0
3	0.0	0.0	0.0	0.08550375081856672	0.0
4	0.0	0.0	0.0	0.12294709951826133	0.0
5	0.0	0.0	0.0	0.22814507919835572	0.0
6	0.0	0.0	0.0	0.3264541310873808	0.0
7	0.0	0.0	0.0	0.3982610725331155	0.0
8	0.0	0.0	0.0	0.5356343973079691	0.0
9	0.0	0.0	0.0	0.5860451394967354	0.0
10	0.0	0.0	0.0	0.6767358477109309	0.0
11	0.0	0.0	0.0	0.8126503407182639	0.0
12	0.0	0.0	0.0	0.9189019211193454	0.0
13	0.0	0.0	0.0	0.961208042377442	0.0
14	0.0	0.0	0.0	0.9828473802623303	0.0
15	8.104620930669831E-5	0.0	0.0	1.018669804775891	0.0
16	8.104620930669831E-5	0.0	0.0	1.0924218552449865	0.0
17	8.104620930669831E-5	0.0	0.0	1.177439328807713	0.0
18	8.104620930669831E-5	0.0	0.0	1.2732359482082305	0.0
19	8.104620930669831E-5	0.0	0.0	1.3354794369557748	0.0
20	8.104620930669831E-5	0.0	0.0	1.4055844080060687	0.0
21	8.104620930669831E-5	0.0	0.0	1.4931143140573029	0.0
22	8.104620930669831E-5	0.0	0.0	1.5925580128766217	0.0
23	8.104620930669831E-5	0.0	0.0	1.7038344582547185	0.0
24	8.104620930669831E-5	0.0	0.0	1.7827734661194428	0.0
25	8.104620930669831E-5	0.0	0.0	1.8543372689372573	0.0
26	8.104620930669831E-5	0.0	0.0	1.9274409497318992	0.0
27	8.104620930669831E-5	0.0	0.0	2.0054074030849427	0.0
28	1.6209241861339662E-4	0.0	0.0	2.0906680152755897	0.0
29	1.6209241861339662E-4	0.0	0.0	2.1863835884668004	0.0
30	1.6209241861339662E-4	0.0	0.0	2.3001724663334047	0.0
31	1.6209241861339662E-4	0.0	0.0	2.405127307385579	0.0
32	1.6209241861339662E-4	0.0	0.0	2.499140910181349	0.0
33	1.6209241861339662E-4	0.0	0.0	2.5981793779541342	0.0
34	1.6209241861339662E-4	0.0	0.0	2.7002165554712674	0.0
35	1.6209241861339662E-4	0.0	0.0	2.8183819286404335	0.0
36	1.6209241861339662E-4	0.0	0.0	2.921796891715781	0.0
37	1.6209241861339662E-4	0.0	0.0	3.0287778880006226	0.0
38	1.6209241861339662E-4	0.0	0.0	3.1487262777745357	0.0
39	1.6209241861339662E-4	0.0	0.0	3.3169782082952417	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGCG	45	3.8562575E-10	45.0	1
CGTACGT	20	7.033489E-4	45.0	29
ATGCGCG	45	3.8562575E-10	45.0	1
TCGAATA	30	2.1656324E-6	44.999996	30
CGACGGT	265	0.0	43.301888	28
TTGTACG	80	0.0	42.1875	1
CGTTTTT	4175	0.0	41.874252	1
CCGCTAT	70	0.0	41.785717	20
TCACGAC	270	0.0	41.666664	25
ACGGTCT	270	0.0	41.666664	30
CGTAAGG	185	0.0	41.351353	2
GACGGTC	280	0.0	40.982143	29
CGGTCTA	280	0.0	40.982143	31
GCGTAAG	145	0.0	40.34483	1
TCGGTAG	40	3.458499E-7	39.375	1
GCGGGAT	730	0.0	39.143837	5
GACAACG	35	6.2486615E-6	38.57143	1
CAACCGG	35	6.2486615E-6	38.57143	2
CGACCCG	35	6.2486615E-6	38.57143	32
GCGTTAG	105	0.0	38.57143	1
>>END_MODULE