FastQCFastQC Report
Thu 26 May 2016
SRR1547720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547720_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1449831
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64450.4445345698912494No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24020.1656744820603229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG351.2122291E-745.0000041
TCGTCCG351.2122291E-745.00000434
TCGTAAG351.2122291E-745.0000041
TCGAGTA302.1658961E-645.0000041
TGATCGC207.0338976E-445.038
CGTATCG253.891313E-545.041
ATAGCGC207.0338976E-445.037
CGCTAGC207.0338976E-445.029
TAGGACG207.0338976E-445.01
ACGACGT207.0338976E-445.012
ATCGATT207.0338976E-445.027
TATCGCG700.041.7857171
CGTTTTT42000.041.6785741
TATAGCG603.6379788E-1241.2500041
CGGGTAT1150.041.0869566
AATGCGG1650.040.909092
ATATCGC556.184564E-1140.9090923
TTAGTCG556.184564E-1140.909091
CGTAAGG1650.040.909092
CGGATTA501.0822987E-940.525