##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547720_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1449831
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69606940395122	31.0	31.0	33.0	30.0	34.0
2	32.08144052651654	33.0	31.0	34.0	30.0	34.0
3	32.148291766419675	33.0	31.0	34.0	30.0	34.0
4	35.838968128009405	37.0	35.0	37.0	35.0	37.0
5	35.7739377899907	37.0	35.0	37.0	35.0	37.0
6	35.82646942988528	37.0	35.0	37.0	35.0	37.0
7	36.15183769694537	37.0	35.0	37.0	35.0	37.0
8	36.04296017949678	37.0	35.0	37.0	35.0	37.0
9	37.84579168192707	39.0	38.0	39.0	35.0	39.0
10	37.50161846449689	39.0	37.0	39.0	35.0	39.0
11	37.16182023973829	39.0	37.0	39.0	34.0	39.0
12	36.35793137269103	38.0	35.0	39.0	33.0	39.0
13	36.076301996577534	38.0	35.0	39.0	32.0	39.0
14	36.95838204590742	39.0	35.0	41.0	32.0	41.0
15	37.19799135209552	39.0	35.0	41.0	33.0	41.0
16	37.32741885088676	39.0	35.0	41.0	33.0	41.0
17	37.24007073927927	39.0	35.0	41.0	33.0	41.0
18	37.14437337869035	39.0	35.0	41.0	32.0	41.0
19	37.14078606403091	38.0	35.0	41.0	32.0	41.0
20	37.04935885630808	38.0	35.0	41.0	32.0	41.0
21	36.90340391397342	38.0	35.0	41.0	32.0	41.0
22	36.86195356562248	38.0	35.0	41.0	32.0	41.0
23	36.80837007899542	38.0	35.0	41.0	32.0	41.0
24	36.80271631659138	38.0	35.0	41.0	32.0	41.0
25	36.740520101998094	38.0	35.0	41.0	32.0	41.0
26	36.708849514184756	38.0	35.0	41.0	32.0	41.0
27	36.63006377984745	38.0	35.0	41.0	32.0	41.0
28	36.63490365428798	38.0	35.0	40.0	32.0	41.0
29	36.565534879582515	38.0	35.0	40.0	32.0	41.0
30	36.55741117412995	38.0	35.0	40.0	32.0	41.0
31	36.40564314047637	38.0	35.0	41.0	31.0	41.0
32	36.2282659151308	38.0	35.0	41.0	31.0	41.0
33	36.14420094479977	38.0	35.0	41.0	30.0	41.0
34	35.96540217446034	38.0	35.0	41.0	30.0	41.0
35	35.85886010162564	38.0	35.0	41.0	30.0	41.0
36	35.79960147079211	38.0	35.0	40.0	30.0	41.0
37	35.55197605789916	38.0	35.0	40.0	29.0	41.0
38	35.652313959351126	38.0	35.0	40.0	29.0	41.0
39	35.59549423346583	38.0	35.0	40.0	29.0	41.0
40	35.46890499651339	37.0	35.0	40.0	29.0	41.0
41	35.427042876031756	37.0	35.0	40.0	29.0	41.0
42	35.43793724923801	37.0	35.0	40.0	29.0	41.0
43	35.35079605829921	37.0	34.0	40.0	29.0	41.0
44	35.22070986204599	37.0	34.0	40.0	28.0	41.0
45	35.198153439952655	37.0	34.0	40.0	28.0	41.0
46	35.04815250880965	36.0	34.0	40.0	27.0	41.0
47	34.9334370695619	36.0	34.0	40.0	27.0	41.0
48	34.81342032278245	36.0	34.0	40.0	27.0	41.0
49	34.884069936427075	36.0	34.0	40.0	27.0	41.0
50	34.76438495245308	36.0	34.0	40.0	27.0	41.0
51	34.17784072764343	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	3.0
13	9.0
14	19.0
15	44.0
16	115.0
17	216.0
18	523.0
19	1014.0
20	1695.0
21	2723.0
22	4442.0
23	6342.0
24	9386.0
25	13793.0
26	18465.0
27	21973.0
28	23929.0
29	26166.0
30	31019.0
31	37958.0
32	48583.0
33	65937.0
34	128614.0
35	219784.0
36	86415.0
37	114829.0
38	190765.0
39	394950.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.07773871575377	23.751733822769687	30.26676902342411	13.903758438052435
2	30.926570062303817	25.54497731114868	30.184414597287546	13.344038029259961
3	29.924246343194483	24.134606033392856	32.039044550709704	13.902103072702957
4	27.21737912901573	26.655589513536405	30.877460890269283	15.24957046717859
5	23.85581491911816	31.483255634622243	29.673389519192238	14.987539927067361
6	22.325153759300225	40.416158848858935	26.404249874640563	10.85443751720028
7	87.83782385671157	3.9760496223352924	6.455235127404505	1.7308913935486274
8	88.99202734663557	3.11119020078892	6.188031570576157	1.7087508819993502
9	85.31394348720644	5.075901949951408	7.139935620082616	2.4702189427595354
10	49.584744704727655	27.79068732838517	13.403631181841194	9.220936785045982
11	42.827198480374605	20.9384404113307	22.284252440456854	13.950108667837839
12	39.820020402377935	20.699102171218577	24.974772921809507	14.506104504593983
13	26.087730225108995	32.985430715717904	24.49575157380412	16.431087485368984
14	17.220007021508025	36.66565275539011	28.564294735041535	17.55004548806033
15	17.016121189297234	26.16015245914869	42.26368452598958	14.560041825564497
16	21.95918006995298	22.031533330436446	40.4489902616236	15.560296337986978
17	22.337500025865083	21.707150695494853	27.604113858787677	28.351235419852383
18	23.388588049227806	25.83611469198824	32.27224414431751	18.503053114466443
19	28.22749685997885	26.532195821444017	26.498674673117073	18.741632645460058
20	30.500313484813056	26.498674673117073	25.104443207518667	17.896568634551198
21	25.187487369217514	27.462097306513655	29.83782247724045	17.512592847028376
22	23.691037093288806	22.761480476000308	29.607105931656864	23.94037649905403
23	20.75097028550224	27.743302495256344	29.056352085174066	22.449375134067353
24	22.04208628453937	24.782267726376382	35.445786439936796	17.729859549147452
25	20.490871004965406	25.487384391698065	32.62704411755577	21.394700485780756
26	18.568371072214624	32.07829050420359	27.693227693434615	21.66011073014717
27	17.742481709937227	32.49254568291063	31.11335045256999	18.651622154582153
28	15.771355420045508	29.426809055676145	35.92087629523717	18.880959229041178
29	18.07155454670234	26.90968809468138	35.06112091685169	19.957636441764592
30	20.23966931318202	28.901230557216667	32.84272442788159	18.016375701719717
31	28.524910834435186	25.697064002632032	27.52520811046253	18.252817052470252
32	28.766939043240214	26.95458987978599	27.455682765784427	16.822788311189374
33	27.4751333086408	26.78201804210284	26.765464388608052	18.97738426064831
34	20.88636537637835	27.898217102545054	29.58517234077627	21.630245180300324
35	19.4764079399599	28.673962689444494	31.218811020043024	20.63081835055258
36	27.93815279160123	26.404111927528106	28.156040255726356	17.501695025144308
37	21.53582038182381	31.810811053150335	28.665892783365788	17.98747578166007
38	21.517818283648232	32.00566134949522	25.058230924845727	21.418289442010828
39	20.847395317109374	30.18779430154273	27.237174539653243	21.727635841694653
40	24.10639584889549	26.517573427523622	26.65634822265492	22.71968250092597
41	19.013871271893066	25.571463156740336	27.94836087792301	27.466304693443583
42	21.47712388547355	26.367280048502206	26.41990687190438	25.735689194119864
43	22.341638439238782	25.82087153606179	26.506951499864467	25.33053852483496
44	19.929495230823456	29.261479441396965	28.63037140190822	22.178653925871362
45	20.032403776716045	33.610951897152155	24.616386323647376	21.740258002484428
46	22.69478304712756	30.914223795738955	26.59930709165413	19.791686065479354
47	21.72694610613237	28.675204213456603	27.060602235708853	22.537247444702178
48	22.85735371915761	26.67448826794295	29.93341982617284	20.534738186726592
49	22.90225550426222	24.120604401478516	30.6011528240188	22.375987270240465
50	20.317471484607516	30.150410634067004	28.165765527154544	21.366352354170935
51	18.968486671894862	31.736940374429846	27.401952365482597	21.892620588192692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1035.0
1	1763.0
2	2491.0
3	7437.5
4	12384.0
5	8834.0
6	5284.0
7	5390.5
8	5497.0
9	5826.0
10	6155.0
11	6264.0
12	6373.0
13	6103.0
14	5833.0
15	5475.0
16	5117.0
17	5022.5
18	4928.0
19	5158.5
20	5389.0
21	5175.0
22	4961.0
23	5725.0
24	6489.0
25	7375.0
26	10006.0
27	11751.0
28	14705.5
29	17660.0
30	21341.5
31	25023.0
32	30188.5
33	35354.0
34	38025.0
35	40696.0
36	44975.0
37	49254.0
38	54458.5
39	59663.0
40	77136.5
41	94610.0
42	112657.5
43	130705.0
44	134423.5
45	138142.0
46	132699.0
47	127256.0
48	119455.0
49	111654.0
50	104832.5
51	98011.0
52	95696.0
53	93381.0
54	86062.0
55	78743.0
56	72659.5
57	66576.0
58	61753.0
59	56930.0
60	53037.5
61	49145.0
62	41318.0
63	33491.0
64	27639.5
65	21788.0
66	17608.0
67	13428.0
68	10428.0
69	7428.0
70	5892.5
71	4357.0
72	3204.5
73	2052.0
74	1650.5
75	979.0
76	709.0
77	518.5
78	328.0
79	215.5
80	103.0
81	91.5
82	80.0
83	51.0
84	22.0
85	16.0
86	10.0
87	10.5
88	11.0
89	11.5
90	12.0
91	9.5
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1449831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.808489402158656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14584921767984	14.450849958222156
2	5.8038493721061295	2.067155800697544
3	1.7560796011789566	0.9381937490082733
4	0.9322194204377796	0.6640567867741074
5	0.6340134421276629	0.5645410832478308
6	0.48193908111706485	0.5149564211135599
7	0.41788726233179097	0.5209358617772956
8	0.3221059506482114	0.45889763267927297
9	0.29294933971869686	0.469528669057481
>10	4.680564210947142	21.008258000927345
>50	2.4310351246102404	31.60955863920701
>100	1.0979999899083321	25.764127411468447
>500	0.002728434479715416	0.3548406911318983
>1k	3.897763542450594E-4	0.16673070033232
>5k	3.897763542450594E-4	0.4473685943554547
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6445	0.4445345698912494	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2402	0.1656744820603229	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.897355622827764E-5	0.0	0.0	0.0111047425527527	0.0
2	6.897355622827764E-5	0.0	0.0	0.051247352277610285	0.0
3	6.897355622827764E-5	0.0	0.0	0.07718140941944268	0.0
4	6.897355622827764E-5	0.0	0.0	0.11987604072474654	0.0
5	6.897355622827764E-5	0.0	0.0	0.22278458661733677	0.0
6	6.897355622827764E-5	0.0	0.0	0.3124502097140977	0.0
7	6.897355622827764E-5	0.0	0.0	0.37494025165691724	0.0
8	6.897355622827764E-5	0.0	0.0	0.49033301122682577	0.0
9	6.897355622827764E-5	0.0	0.0	0.5355106905563476	0.0
10	6.897355622827764E-5	0.0	0.0	0.6229001862975754	0.0
11	6.897355622827764E-5	0.0	0.0	0.764916738571599	0.0
12	6.897355622827764E-5	0.0	0.0	0.8725844598439404	0.0
13	6.897355622827764E-5	0.0	0.0	0.9172793242798644	0.0
14	6.897355622827764E-5	0.0	0.0	0.9370057613611518	0.0
15	6.897355622827764E-5	0.0	0.0	0.9666643905393111	0.0
16	6.897355622827764E-5	0.0	0.0	1.0296372473757287	0.0
17	6.897355622827764E-5	0.0	0.0	1.10205948141542	0.0
18	6.897355622827764E-5	0.0	0.0	1.19096639539367	0.0
19	6.897355622827764E-5	0.0	0.0	1.2510423628685	0.0
20	6.897355622827764E-5	0.0	0.0	1.3215333373337996	0.0
21	6.897355622827764E-5	0.0	0.0	1.411336907543017	0.0
22	6.897355622827764E-5	0.0	0.0	1.5003127950774953	0.0
23	6.897355622827764E-5	0.0	0.0	1.5911509686301368	0.0
24	6.897355622827764E-5	0.0	0.0	1.6580553181715663	0.0
25	6.897355622827764E-5	0.0	0.0	1.7149585020598952	0.0
26	6.897355622827764E-5	0.0	0.0	1.7797936449144762	0.0
27	6.897355622827764E-5	0.0	0.0	1.858492472570941	0.0
28	6.897355622827764E-5	0.0	0.0	1.9281557643615015	0.0
29	6.897355622827764E-5	0.0	0.0	2.0176144667895777	0.0
30	6.897355622827764E-5	0.0	0.0	2.1045901211934357	0.0
31	6.897355622827764E-5	0.0	0.0	2.1905311722538694	0.0
32	6.897355622827764E-5	0.0	0.0	2.2646087716430396	0.0
33	6.897355622827764E-5	0.0	0.0	2.3477219068981143	0.0
34	6.897355622827764E-5	0.0	0.0	2.442560546711996	0.0
35	6.897355622827764E-5	0.0	0.0	2.553263104458382	0.0
36	6.897355622827764E-5	0.0	0.0	2.6434115424487405	0.0
37	6.897355622827764E-5	0.0	0.0	2.740940150955525	0.0
38	6.897355622827764E-5	0.0	0.0	2.8639200017105444	0.0
39	6.897355622827764E-5	0.0	0.0	3.096153965531155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGTCG	35	1.2122291E-7	45.000004	1
TCGTCCG	35	1.2122291E-7	45.000004	34
TCGTAAG	35	1.2122291E-7	45.000004	1
TCGAGTA	30	2.1658961E-6	45.000004	1
TGATCGC	20	7.0338976E-4	45.0	38
CGTATCG	25	3.891313E-5	45.0	41
ATAGCGC	20	7.0338976E-4	45.0	37
CGCTAGC	20	7.0338976E-4	45.0	29
TAGGACG	20	7.0338976E-4	45.0	1
ACGACGT	20	7.0338976E-4	45.0	12
ATCGATT	20	7.0338976E-4	45.0	27
TATCGCG	70	0.0	41.785717	1
CGTTTTT	4200	0.0	41.678574	1
TATAGCG	60	3.6379788E-12	41.250004	1
CGGGTAT	115	0.0	41.086956	6
AATGCGG	165	0.0	40.90909	2
ATATCGC	55	6.184564E-11	40.90909	23
TTAGTCG	55	6.184564E-11	40.90909	1
CGTAAGG	165	0.0	40.90909	2
CGGATTA	50	1.0822987E-9	40.5	25
>>END_MODULE