FastQCFastQC Report
Thu 26 May 2016
SRR1547719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547719_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2321423
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129010.5557367183835088No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37750.16261577489324436No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA207.034774E-445.00000441
CGCATAA207.034774E-445.00000444
ATTACGA207.034774E-445.00000420
CCGCTAT207.034774E-445.00000425
ATCCGGC207.034774E-445.00000435
ATATCGT207.034774E-445.00000413
GTGTCGA406.8193913E-945.00000444
CGAATCG207.034774E-445.00000422
TCTCCGA207.034774E-445.00000436
CCGGTAT207.034774E-445.00000427
TAGTCCG207.034774E-445.0000041
AATCGGC207.034774E-445.00000424
GTACGAC207.034774E-445.00000444
CTAGACG253.8920396E-545.01
GTCGCGA351.2126475E-745.016
GCGCGTC453.8562575E-1045.038
CTATCCG351.2126475E-745.01
TAACGCG453.8562575E-1045.01
TCGGCGA253.8920396E-545.034
GCCGATC253.8920396E-545.09