##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547719_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2321423
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64046793712305	31.0	31.0	33.0	30.0	34.0
2	32.00982673127646	33.0	31.0	34.0	30.0	34.0
3	32.05668893605345	33.0	31.0	34.0	30.0	34.0
4	35.75266765255621	37.0	35.0	37.0	35.0	37.0
5	35.67228893657037	37.0	35.0	37.0	33.0	37.0
6	35.73722152317781	37.0	35.0	37.0	33.0	37.0
7	36.13869380978822	37.0	35.0	37.0	35.0	37.0
8	36.050237289800265	37.0	35.0	37.0	35.0	37.0
9	37.865155553296404	39.0	38.0	39.0	35.0	39.0
10	37.450918682204836	39.0	37.0	39.0	35.0	39.0
11	37.09341167034186	39.0	37.0	39.0	33.0	39.0
12	36.26886741451256	38.0	35.0	39.0	33.0	39.0
13	35.96004648872696	38.0	35.0	39.0	32.0	39.0
14	36.88249879492018	39.0	35.0	41.0	32.0	41.0
15	37.13958464269545	39.0	35.0	41.0	32.0	41.0
16	37.295897387076806	39.0	35.0	41.0	33.0	41.0
17	37.22669802099833	39.0	35.0	41.0	33.0	41.0
18	37.140658983735406	38.0	35.0	41.0	32.0	41.0
19	37.127059997251685	38.0	35.0	41.0	32.0	41.0
20	37.00750832571229	38.0	35.0	41.0	32.0	41.0
21	36.84972880857991	38.0	35.0	41.0	32.0	41.0
22	36.7998658581396	38.0	35.0	41.0	32.0	41.0
23	36.740432053960006	38.0	35.0	41.0	32.0	41.0
24	36.73183732564035	38.0	35.0	41.0	32.0	41.0
25	36.653156706037635	38.0	35.0	41.0	32.0	41.0
26	36.612761655243354	38.0	35.0	40.0	32.0	41.0
27	36.51710782567417	38.0	35.0	40.0	31.0	41.0
28	36.55343382054886	38.0	35.0	40.0	32.0	41.0
29	36.509643007758605	38.0	35.0	40.0	31.0	41.0
30	36.511790397527726	38.0	35.0	40.0	31.0	41.0
31	36.349485207995265	38.0	35.0	40.0	31.0	41.0
32	36.13649171219549	38.0	35.0	40.0	31.0	41.0
33	36.0554513330832	38.0	35.0	41.0	30.0	41.0
34	35.90847079571453	38.0	35.0	40.0	30.0	41.0
35	35.79456652234427	38.0	35.0	40.0	30.0	41.0
36	35.704995599681745	38.0	35.0	40.0	30.0	41.0
37	35.43304473161505	37.0	34.0	40.0	29.0	41.0
38	35.563532798632565	38.0	35.0	40.0	29.0	41.0
39	35.518798168192525	37.0	35.0	40.0	29.0	41.0
40	35.4185256198461	37.0	35.0	40.0	29.0	41.0
41	35.386689543439516	37.0	35.0	40.0	29.0	41.0
42	35.40199868787377	37.0	35.0	40.0	29.0	41.0
43	35.31791620915275	37.0	34.0	40.0	28.0	41.0
44	35.195619238716944	37.0	34.0	40.0	28.0	41.0
45	35.17627463844375	37.0	34.0	40.0	28.0	41.0
46	34.9919682884162	36.0	34.0	40.0	27.0	41.0
47	34.86258773174902	36.0	34.0	40.0	27.0	41.0
48	34.751064325631305	36.0	34.0	40.0	26.0	41.0
49	34.84055684810566	36.0	34.0	40.0	27.0	41.0
50	34.70510975380187	36.0	34.0	40.0	26.0	41.0
51	34.083427277148544	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	5.0
13	15.0
14	20.0
15	52.0
16	121.0
17	336.0
18	708.0
19	1544.0
20	2636.0
21	4363.0
22	7050.0
23	10604.0
24	15330.0
25	23074.0
26	30530.0
27	35736.0
28	38864.0
29	42938.0
30	50483.0
31	62975.0
32	80045.0
33	107178.0
34	201021.0
35	371627.0
36	139934.0
37	180521.0
38	292538.0
39	621007.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.219256464677052	24.64445299284103	30.607476534866763	13.528814007615155
2	29.66572658235918	25.737489462282404	30.99616054463146	13.600623410726955
3	28.08376586257653	25.628547662360546	33.52340353309155	12.76428294197137
4	26.247866071801653	27.29455166077014	31.94432897408185	14.513253293346365
5	23.666647569184935	31.50976793113534	30.963249696414657	13.860334803265067
6	21.355866638695318	41.665521535713225	26.780858120213335	10.197753705378124
7	89.35415906536636	3.2225492725797924	6.030783704650123	1.3925079574037131
8	90.39683849087392	2.596381615931263	5.619053485728365	1.3877264074664548
9	87.1197537027935	4.595801799155088	6.5105756253815015	1.77386887266991
10	48.85356955625924	33.03086081252749	11.012211044691123	7.1033585865221465
11	40.962461386830405	20.976745728805135	24.478864903121924	13.58192798124254
12	38.31227656484837	21.867406327929036	25.787286504872224	14.033030602350369
13	23.186597186294787	37.08910439846594	25.59662758575236	14.127670829486913
14	14.43020078632804	42.24434754028025	27.896553105573606	15.4288985678181
15	13.291330360731326	28.887453945274082	44.938772468438536	12.882443225556047
16	17.01900084560203	23.890174259495144	44.17661063925015	14.914214255652675
17	18.146972783503912	23.413871577907173	28.135932141621755	30.303223496967163
18	20.918419434975874	26.277287680875048	34.64892008048512	18.155372803663962
19	26.187945927993304	27.898750033923157	27.1156958469008	18.797608191182736
20	29.18317773193425	26.70982410357785	26.6637747622902	17.443223402197706
21	22.745531512352553	28.486320674861926	30.859821755879906	17.90832605690561
22	21.926809547419836	23.512345660398815	29.95356727317684	24.60727751900451
23	17.577494493679094	30.043899797667205	29.235559396111782	23.14304631254192
24	18.445625807963477	26.765695006898788	37.73689672239829	17.05178246273945
25	16.78147412169174	27.91641161477249	35.75522427407672	19.54688998945905
26	16.498888828102416	35.91732312465242	27.648472510180177	19.935315537064984
27	16.140014120649273	36.42679511661597	31.473755537013286	15.959435225721466
28	14.0371659968907	31.03910834001386	38.89730566122589	16.026420001869543
29	15.094276226262943	26.612814639985906	38.260541056067765	20.032368077683387
30	17.430903372629633	32.210588074642146	33.15134725554111	17.207161297187113
31	27.032514108803092	28.94018022566331	27.152440550472708	16.874865115060892
32	26.920513840002446	28.539003878224694	28.36841885343602	16.172063428336845
33	25.479673458908607	29.448230675753624	26.544322167911666	18.527773697426106
34	18.997485593965425	30.651415101857783	28.548653132152136	21.80244617202466
35	17.562417534417467	29.07134977123945	31.9258920067562	21.440340687586882
36	28.06976582897645	26.110321126309167	28.839595368875038	16.980317675839345
37	19.01639640858215	31.747208500992706	31.377822999082888	17.85857209134225
38	18.78421123595312	33.266233685114685	26.58115302553649	21.368402053395698
39	18.34185325121703	31.600272763731557	30.301888109146844	19.755985875904564
40	22.61091580465947	28.671853427832843	27.126637411622095	21.59059335588559
41	17.84078127941353	26.12492423827971	29.143503790562942	26.890790691743817
42	20.93685640230152	26.894323007913677	26.744587263932512	25.424233325852292
43	21.783276895249166	26.86373831912581	27.647180199386323	23.7058045862387
44	18.44105964315853	31.369379901896384	29.771911452587485	20.417649002357603
45	17.51981435524676	37.59095175674575	24.706139294734307	20.183094593273175
46	22.781026982157066	32.52181959082856	26.949806217996464	17.747347209017917
47	20.578369388086532	30.449900772069544	26.93382464117914	22.037905198664784
48	21.348371236090966	27.389278041959606	30.925557298260593	20.33679342368883
49	20.76446214240145	25.928449920587504	31.545694171204474	21.761393765806577
50	19.606939364346783	32.469739465836255	28.651564148369342	19.271757021447623
51	17.642928496874546	34.24059294665384	26.38476486189721	21.731713694574406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1688.0
1	2403.5
2	3119.0
3	11362.5
4	19606.0
5	13667.0
6	7728.0
7	8263.0
8	8798.0
9	10046.0
10	11294.0
11	11871.5
12	12449.0
13	12428.0
14	12407.0
15	12333.5
16	12260.0
17	11615.5
18	10971.0
19	10671.0
20	10371.0
21	10757.0
22	11143.0
23	12533.0
24	13923.0
25	16902.5
26	23693.0
27	27504.0
28	32142.0
29	36780.0
30	46507.0
31	56234.0
32	61689.5
33	67145.0
34	75839.5
35	84534.0
36	89997.5
37	95461.0
38	105864.0
39	116267.0
40	145115.5
41	173964.0
42	215932.5
43	257901.0
44	251779.5
45	245658.0
46	234331.5
47	223005.0
48	210342.5
49	197680.0
50	179484.0
51	161288.0
52	141814.5
53	122341.0
54	105254.0
55	88167.0
56	79834.5
57	71502.0
58	63422.0
59	55342.0
60	45074.5
61	34807.0
62	27996.5
63	21186.0
64	17736.5
65	14287.0
66	10573.5
67	6860.0
68	5406.0
69	3952.0
70	2943.5
71	1935.0
72	1380.5
73	826.0
74	663.5
75	386.5
76	272.0
77	215.0
78	158.0
79	145.0
80	132.0
81	82.0
82	32.0
83	25.5
84	19.0
85	18.0
86	17.0
87	15.0
88	13.0
89	9.0
90	5.0
91	4.5
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2321423.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.503273801447452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1538181735379	15.016113176978541
2	8.014419061553811	2.9658598051093943
3	2.5235633200698557	1.4008254919962695
4	1.1916106665269497	0.8819479370989378
5	0.7493835848323169	0.6933024826231292
6	0.48435518390357557	0.5377293950950975
7	0.3501147984623004	0.4534788984520577
8	0.2660060628213805	0.39375864105832287
9	0.2312816118716669	0.38515202907313967
>10	2.430928153338887	10.50074665946334
>50	0.9927110628748931	13.416089840324133
>100	1.601295003156919	51.362049375890464
>500	0.009345170710774942	1.1153530569317434
>1k	9.345170710774943E-4	0.31989506690806174
>5k	0.0	0.0
>10k+	2.3362926776937358E-4	0.5576981429974195
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12901	0.5557367183835088	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3775	0.16261577489324436	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04393856699102232	0.0
2	0.0	0.0	0.0	0.1625296208403208	0.0
3	0.0	0.0	0.0	0.2237851524689813	0.0
4	0.0	0.0	0.0	0.32036384579630683	0.0
5	0.0	0.0	0.0	0.5749059951590038	0.0
6	0.0	0.0	0.0	0.787189581562688	0.0
7	0.0	0.0	0.0	0.9459715011008334	0.0
8	0.0	0.0	0.0	1.2905877127951262	0.0
9	0.0	0.0	0.0	1.3940156533298758	0.0
10	0.0	0.0	0.0	1.5883791967254568	0.0
11	0.0	0.0	0.0	1.9293338611704975	0.0
12	0.0	0.0	0.0	2.1800421551780955	0.0
13	0.0	0.0	0.0	2.2698146783244586	0.0
14	0.0	0.0	0.0	2.3041470684145025	0.0
15	0.0	0.0	0.0	2.3659626013871664	0.0
16	0.0	0.0	0.0	2.5055752441498167	0.0
17	0.0	0.0	0.0	2.691237228200117	0.0
18	0.0	0.0	0.0	2.904597740265346	0.0
19	0.0	0.0	0.0	3.034216512888862	0.0
20	0.0	0.0	0.0	3.167798371946862	0.0
21	0.0	0.0	0.0	3.345792645286964	0.0
22	0.0	0.0	0.0	3.535546946851134	0.0
23	0.0	0.0	0.0	3.736415121242445	0.0
24	0.0	0.0	0.0	3.881713931498051	0.0
25	0.0	0.0	0.0	4.004569610967066	0.0
26	0.0	0.0	0.0	4.132077609293955	0.0
27	0.0	0.0	0.0	4.259370222488534	0.0
28	0.0	0.0	0.0	4.387136682974193	0.0
29	0.0	0.0	0.0	4.540361666098768	0.0
30	0.0	0.0	0.0	4.720165174550265	0.0
31	0.0	0.0	0.0	4.8914825087887905	0.0
32	0.0	0.0	0.0	5.036781319044397	0.0
33	0.0	0.0	0.0	5.1862155238403345	0.0
34	0.0	0.0	0.0	5.340000508308912	0.0
35	0.0	0.0	0.0	5.5525425568713676	0.0
36	0.0	0.0	0.0	5.725410664062517	0.0
37	0.0	0.0	0.0	5.894186453739796	0.0
38	0.0	0.0	0.0	6.071146878444816	0.0
39	0.0	0.0	0.0	6.304925901052932	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	20	7.034774E-4	45.000004	41
CGCATAA	20	7.034774E-4	45.000004	44
ATTACGA	20	7.034774E-4	45.000004	20
CCGCTAT	20	7.034774E-4	45.000004	25
ATCCGGC	20	7.034774E-4	45.000004	35
ATATCGT	20	7.034774E-4	45.000004	13
GTGTCGA	40	6.8193913E-9	45.000004	44
CGAATCG	20	7.034774E-4	45.000004	22
TCTCCGA	20	7.034774E-4	45.000004	36
CCGGTAT	20	7.034774E-4	45.000004	27
TAGTCCG	20	7.034774E-4	45.000004	1
AATCGGC	20	7.034774E-4	45.000004	24
GTACGAC	20	7.034774E-4	45.000004	44
CTAGACG	25	3.8920396E-5	45.0	1
GTCGCGA	35	1.2126475E-7	45.0	16
GCGCGTC	45	3.8562575E-10	45.0	38
CTATCCG	35	1.2126475E-7	45.0	1
TAACGCG	45	3.8562575E-10	45.0	1
TCGGCGA	25	3.8920396E-5	45.0	34
GCCGATC	25	3.8920396E-5	45.0	9
>>END_MODULE