FastQCFastQC Report
Thu 26 May 2016
SRR1547718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547718_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2601674
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183970.7071216455251503No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA99270.3815620250654002No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39830.15309373887735359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGC406.8212103E-945.00000412
ACGTTAG700.045.0000041
GTCGTAC207.0349313E-445.00000420
CGGTCTA7250.043.44827731
CGTTTTT98800.043.0642741
CATTAGA15400.041.05519517
TATAGCG1600.040.7812541
CACGACG8000.040.7812526
TCACGAC7950.040.471725
CTCACGA7950.040.471724
TAACGCG950.040.263161
ATATACG451.9299478E-840.01
TACGTAC451.9299478E-840.024
TTACGGG8000.039.93753
ACGGTCT7950.039.90566330
TATTACG850.039.7058831
CGTACAA850.039.70588336
TCTCACG8150.039.47852723
TAGCGAG2750.039.2727281
GCGATAA1950.039.230779
Produced by FastQC (version 0.11.5)