##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547718_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2601674
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60950680215892	31.0	31.0	33.0	30.0	34.0
2	31.9900302651293	33.0	31.0	34.0	30.0	34.0
3	32.05206801467055	33.0	31.0	34.0	30.0	34.0
4	35.76617670007849	37.0	35.0	37.0	35.0	37.0
5	35.701796996856636	37.0	35.0	37.0	33.0	37.0
6	35.76100772041386	37.0	35.0	37.0	33.0	37.0
7	36.09607083746849	37.0	35.0	37.0	35.0	37.0
8	35.99019938701006	37.0	35.0	37.0	35.0	37.0
9	37.77660959828172	39.0	38.0	39.0	35.0	39.0
10	37.4334397776201	39.0	37.0	39.0	35.0	39.0
11	37.105820329526296	39.0	37.0	39.0	33.0	39.0
12	36.494900206559315	39.0	35.0	39.0	33.0	39.0
13	36.26245794054136	39.0	35.0	39.0	32.0	39.0
14	37.25151998290332	39.0	35.0	41.0	32.0	41.0
15	37.46954653042618	39.0	35.0	41.0	33.0	41.0
16	37.5698550241114	40.0	35.0	41.0	33.0	41.0
17	37.47401288554984	39.0	35.0	41.0	33.0	41.0
18	37.365975906281875	39.0	35.0	41.0	32.0	41.0
19	37.32437961097355	39.0	35.0	41.0	32.0	41.0
20	37.20729653292457	39.0	35.0	41.0	32.0	41.0
21	37.06656099111572	39.0	35.0	41.0	32.0	41.0
22	37.01922416105938	39.0	35.0	41.0	32.0	41.0
23	36.91485174545312	38.0	35.0	41.0	32.0	41.0
24	36.91512656850935	38.0	35.0	41.0	32.0	41.0
25	36.834691433284874	38.0	35.0	41.0	32.0	41.0
26	36.809877025330614	38.0	35.0	41.0	32.0	41.0
27	36.71546934781222	38.0	35.0	41.0	31.0	41.0
28	36.71547549769879	38.0	35.0	41.0	32.0	41.0
29	36.66163708443102	38.0	35.0	41.0	31.0	41.0
30	36.659031454363614	38.0	35.0	41.0	31.0	41.0
31	36.51881711544183	38.0	35.0	41.0	31.0	41.0
32	36.32617614658869	38.0	35.0	41.0	30.0	41.0
33	36.23145789979836	38.0	35.0	41.0	30.0	41.0
34	36.03508241232376	38.0	35.0	41.0	30.0	41.0
35	35.913105946402204	38.0	35.0	41.0	30.0	41.0
36	35.814279959748994	38.0	35.0	41.0	29.0	41.0
37	35.570192114769185	38.0	35.0	40.0	28.0	41.0
38	35.664345725098535	38.0	35.0	40.0	29.0	41.0
39	35.59308852684848	38.0	35.0	40.0	29.0	41.0
40	35.476596222278424	38.0	35.0	40.0	28.0	41.0
41	35.423066456443046	38.0	35.0	40.0	28.0	41.0
42	35.43342786221486	38.0	35.0	40.0	28.0	41.0
43	35.33480520618648	38.0	34.0	40.0	27.0	41.0
44	35.183923504635864	37.0	34.0	40.0	27.0	41.0
45	35.16867678271759	37.0	34.0	40.0	27.0	41.0
46	34.98853738016369	37.0	34.0	40.0	26.0	41.0
47	34.89180389241696	37.0	34.0	40.0	26.0	41.0
48	34.75136123895615	37.0	34.0	40.0	26.0	41.0
49	34.82399255248736	36.0	34.0	40.0	26.0	41.0
50	34.69868899792979	36.0	34.0	40.0	26.0	41.0
51	34.08335517824293	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	10.0
12	20.0
13	26.0
14	40.0
15	108.0
16	205.0
17	501.0
18	1037.0
19	1989.0
20	3299.0
21	5440.0
22	8400.0
23	11911.0
24	17659.0
25	25527.0
26	35154.0
27	41622.0
28	44928.0
29	48871.0
30	56584.0
31	69027.0
32	86469.0
33	114090.0
34	206565.0
35	337063.0
36	167826.0
37	226003.0
38	367369.0
39	723776.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15915060841596	23.46147134498788	29.76706535868829	13.612312687907863
2	31.743254535349163	24.72023781611378	30.0041050492875	13.53240259924956
3	29.015241725135432	24.839007500555415	32.55354052813688	13.592210246172273
4	26.357568242600728	27.00641971284642	31.562486306893177	15.073525737659676
5	23.584891881150366	32.23866633559777	30.308755055398944	13.867686727852913
6	22.425253894223488	40.31942510860315	26.91824571410561	10.337075283067747
7	86.61734713880371	3.8920325913238933	6.969282085303539	2.5213381845688585
8	87.47794689111703	2.9879223915063915	7.730983974164326	1.8031467432122548
9	83.0141670324568	5.39564142163853	8.636170404132109	2.954021141772566
10	46.57804936360205	32.4858149022514	12.458978334718338	8.477157399428215
11	36.52625194394071	21.99422371903628	25.419479919467236	16.060044417555773
12	34.58696208671802	20.685681603459926	27.579973509363587	17.147382800458473
13	24.819250989939555	31.093864950028326	26.65456932728697	17.432314732745148
14	17.459412670457557	34.47257419645967	29.07028320996405	18.99772992311873
15	16.003503897874985	26.80839336519487	40.412557453393475	16.775545283536676
16	21.735198183938493	22.963561153318977	38.27381908724921	17.027421575493317
17	21.9159279756034	22.028394026307677	28.36569839265027	27.68997960543865
18	22.628546082253195	26.337619548029462	31.923753706267583	19.110080663449764
19	26.718681894810803	26.37378856843709	28.024725618966865	18.88280391778524
20	28.536357745051838	26.23530081017068	27.29150539229742	17.936836052480057
21	26.067255159562652	26.006025351369928	29.999723255104215	17.92699623396321
22	25.359633835753442	21.23678831398553	29.384042735561795	24.019535114699227
23	20.0488992856138	27.384599300296653	30.153739476967523	22.41276193712202
24	21.247281557950764	25.681772581806943	34.65165120610807	18.419294654134223
25	20.587052797544967	26.58030944691764	32.40148458261873	20.431153172918666
26	19.633858815516472	31.78530438479225	27.13472172147625	21.446115078215026
27	18.441818613707945	30.972289379837754	31.61360723903148	18.97228476742282
28	17.319310566965733	27.937896907913906	35.34470498609741	19.398087539022953
29	17.717900090480207	26.63804150712195	35.238542569130495	20.40551583326735
30	19.683519149593685	28.469016487077166	32.90573684481607	18.94172751851308
31	27.519896804903304	25.57341926774838	27.273401663698067	19.63328226365025
32	26.902909434464117	27.17058324755523	27.77219590156184	18.154311416418814
33	25.1547657392894	26.635081874208684	27.4660852973893	20.744067089112626
34	20.59793040942101	27.77234964872617	29.366707742784072	22.263012199068754
35	20.477738563709366	27.206560084007453	30.611329474791997	21.704371877491184
36	26.704767776439326	25.547628180932737	28.436960203315248	19.31064383931269
37	20.733227914027662	30.031472044537477	29.056638149130137	20.178661892304724
38	20.482889093714277	31.512672225651638	25.86680729407297	22.137631386561115
39	20.480621323040474	29.703375595866355	28.128428081304573	21.6875749997886
40	23.083330194328727	26.119682942597727	28.554192416113626	22.24279444695992
41	19.521892443096252	25.178058434684747	28.970116932405826	26.329932189813178
42	21.805691258781845	25.26150470812254	27.145291839023646	25.787512194071972
43	22.698808536350057	25.467487471527946	26.990737502085192	24.8429664900368
44	20.608269906221917	28.11874200995205	29.662094482244893	21.610893601581136
45	19.946964915665838	32.731887238754744	25.72209277565137	21.599055069928056
46	22.506855201689373	30.041619357382977	26.828649554094785	20.622875886832862
47	21.952865731832656	27.752862195647882	27.67814107378557	22.61613099873389
48	22.16745833644031	26.693390486279217	30.05530285500797	21.083848322272505
49	22.10261546988593	25.144080311368754	30.884807243336404	21.86849697540891
50	20.203568932925496	29.766142875702332	29.366707742784072	20.6635804485881
51	20.02237789976761	30.42125954289431	27.85618029007477	21.70018226726331
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1959.0
1	3376.0
2	4793.0
3	17434.5
4	30076.0
5	20927.0
6	11778.0
7	11982.5
8	12187.0
9	12684.0
10	13181.0
11	13108.0
12	13035.0
13	12566.5
14	12098.0
15	11345.5
16	10593.0
17	9487.5
18	8382.0
19	8123.5
20	7865.0
21	9187.5
22	10510.0
23	11626.5
24	12743.0
25	14914.0
26	21207.5
27	25330.0
28	30415.0
29	35500.0
30	44992.5
31	54485.0
32	56360.0
33	58235.0
34	65974.0
35	73713.0
36	80831.0
37	87949.0
38	99796.5
39	111644.0
40	136796.0
41	161948.0
42	190473.0
43	218998.0
44	222862.5
45	226727.0
46	223407.0
47	220087.0
48	212823.0
49	205559.0
50	187919.0
51	170279.0
52	165159.5
53	160040.0
54	149247.5
55	138455.0
56	129246.5
57	120038.0
58	112929.5
59	105821.0
60	94317.5
61	82814.0
62	73477.5
63	64141.0
64	56464.5
65	48788.0
66	37706.5
67	26625.0
68	21689.0
69	16753.0
70	13181.0
71	9609.0
72	7667.0
73	5725.0
74	4390.5
75	2243.0
76	1430.0
77	1133.5
78	837.0
79	625.5
80	414.0
81	323.5
82	233.0
83	143.0
84	53.0
85	45.0
86	37.0
87	40.5
88	44.0
89	26.0
90	8.0
91	6.0
92	4.0
93	5.0
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2601674.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.155909655239093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.41670017046182	14.963501623778333
2	6.5482145943206795	2.3777758515520873
3	1.9334926984221026	1.0531295625484842
4	1.0007063817340127	0.7267493863273576
5	0.6254163400182386	0.5677501283140718
6	0.4331827358781169	0.4718895970087433
7	0.33037805955264143	0.41988199409176713
8	0.25919750173014106	0.37647731394208955
9	0.22263534012533243	0.36379324092410664
>10	3.10067083273724	14.113272744120678
>50	1.6368438968747747	21.732651156635203
>100	1.4840459074492063	40.28341966919359
>500	0.005960878486997141	0.723949092141189
>1k	0.0021288851739275504	0.7309837282741146
>5k	2.1288851739275502E-4	0.3836968840195186
>10k+	2.1288851739275502E-4	0.7110780271287482
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18397	0.7071216455251503	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	9927	0.3815620250654002	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3983	0.15309373887735359	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016028141880958184	0.0
2	0.0	0.0	0.0	0.06253665908949392	0.0
3	0.0	0.0	0.0	0.09413170135843307	0.0
4	0.0	0.0	0.0	0.1344134584117764	0.0
5	0.0	0.0	0.0	0.24299739321682887	0.0
6	0.0	0.0	0.0	0.358192456087888	0.0
7	0.0	0.0	0.0	0.4383716022837604	0.0
8	0.0	0.0	0.0	0.5915422147432768	0.0
9	0.0	0.0	0.0	0.6621890367509534	0.0
10	0.0	0.0	0.0	0.7746166506641493	0.0
11	0.0	0.0	0.0	0.9295169187223303	0.0
12	0.0	0.0	0.0	1.0515537304058848	0.0
13	0.0	0.0	0.0	1.115973792258369	0.0
14	3.843679108143449E-5	0.0	0.0	1.1408423960880572	0.0
15	3.843679108143449E-5	0.0	0.0	1.2034943655507955	0.0
16	3.843679108143449E-5	0.0	0.0	1.273872130020902	0.0
17	7.687358216286899E-5	0.0	0.0	1.361815508015224	0.0
18	7.687358216286899E-5	0.0	0.0	1.476549329393306	0.0
19	7.687358216286899E-5	0.0	0.0	1.5500020371499272	0.0
20	7.687358216286899E-5	0.0	0.0	1.6315649078247314	0.0
21	7.687358216286899E-5	0.0	0.0	1.732461484413497	0.0
22	7.687358216286899E-5	0.0	0.0	1.8430056955637024	0.0
23	7.687358216286899E-5	0.0	0.0	1.9659649902332115	0.0
24	7.687358216286899E-5	0.0	0.0	2.0526783909129276	0.0
25	7.687358216286899E-5	0.0	0.0	2.14946223085598	0.0
26	7.687358216286899E-5	0.0	0.0	2.226720180929663	0.0
27	7.687358216286899E-5	0.0	0.0	2.306784016752291	0.0
28	7.687358216286899E-5	0.0	0.0	2.395419256986079	0.0
29	7.687358216286899E-5	0.0	0.0	2.495739281708623	0.0
30	7.687358216286899E-5	0.0	0.0	2.6178914037654217	0.0
31	7.687358216286899E-5	0.0	0.0	2.7250531773004614	0.0
32	7.687358216286899E-5	0.0	0.0	2.83701954972068	0.0
33	7.687358216286899E-5	0.0	0.0	2.937531758398631	0.0
34	7.687358216286899E-5	0.0	0.0	3.067063744343065	0.0
35	7.687358216286899E-5	0.0	0.0	3.2020537546210632	0.0
36	7.687358216286899E-5	0.0	0.0	3.3096767696490796	0.0
37	7.687358216286899E-5	0.0	0.0	3.4250255796844646	0.0
38	7.687358216286899E-5	0.0	0.0	3.557901566452984	0.0
39	7.687358216286899E-5	0.0	0.0	3.7601175243324105	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGC	40	6.8212103E-9	45.000004	12
ACGTTAG	70	0.0	45.000004	1
GTCGTAC	20	7.0349313E-4	45.000004	20
CGGTCTA	725	0.0	43.448277	31
CGTTTTT	9880	0.0	43.064274	1
CATTAGA	1540	0.0	41.055195	17
TATAGCG	160	0.0	40.781254	1
CACGACG	800	0.0	40.78125	26
TCACGAC	795	0.0	40.4717	25
CTCACGA	795	0.0	40.4717	24
TAACGCG	95	0.0	40.26316	1
ATATACG	45	1.9299478E-8	40.0	1
TACGTAC	45	1.9299478E-8	40.0	24
TTACGGG	800	0.0	39.9375	3
ACGGTCT	795	0.0	39.905663	30
TATTACG	85	0.0	39.705883	1
CGTACAA	85	0.0	39.705883	36
TCTCACG	815	0.0	39.478527	23
TAGCGAG	275	0.0	39.272728	1
GCGATAA	195	0.0	39.23077	9
>>END_MODULE