FastQCFastQC Report
Thu 26 May 2016
SRR1547717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547717_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2248136
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT457732.0360423034905364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG302.166431E-645.0000041
TCACGAC3250.044.30769325
CGTTTTT203450.044.082081
CGCACGG900.042.52
CGGTCTA3350.042.31343531
TAGTACG750.042.01
TCGTCCC2700.041.66666838
CGACGGT3500.041.14285728
GACGGTC3500.041.14285729
CACGACG3500.040.526
ACGGTCT3500.040.530
TGCGTAG2250.040.01
CTATGCG1250.039.61
CTCGTCC2900.039.5689737
GTTTTTT238450.038.649612
GGCGATA4450.038.4269688
TAGGGAT36850.038.40575
AGGGATT45850.038.2769936
GTTAGCG1000.038.251
AAGGGAT35250.038.2340435
Produced by FastQC (version 0.11.5)