##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547717_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2248136
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61991979132935	31.0	31.0	33.0	30.0	34.0
2	31.993897611176546	33.0	31.0	34.0	30.0	34.0
3	31.9515273986983	33.0	31.0	34.0	30.0	34.0
4	35.723545194774694	37.0	35.0	37.0	33.0	37.0
5	35.677065355476714	37.0	35.0	37.0	33.0	37.0
6	35.73866839016856	37.0	35.0	37.0	33.0	37.0
7	36.115028628161284	37.0	35.0	37.0	35.0	37.0
8	36.01637178533683	37.0	35.0	37.0	35.0	37.0
9	37.81506323460858	39.0	38.0	39.0	35.0	39.0
10	37.44639648135166	39.0	37.0	39.0	35.0	39.0
11	37.110099655892704	39.0	37.0	39.0	33.0	39.0
12	36.68204103310476	39.0	35.0	39.0	33.0	39.0
13	36.534694965073285	39.0	35.0	39.0	33.0	39.0
14	37.64296910863044	40.0	35.0	41.0	33.0	41.0
15	37.81097317955853	40.0	36.0	41.0	33.0	41.0
16	37.8836017927741	40.0	35.0	41.0	33.0	41.0
17	37.775042968930705	40.0	35.0	41.0	33.0	41.0
18	37.5670088464399	39.0	36.0	41.0	33.0	41.0
19	37.44466838305156	39.0	36.0	41.0	33.0	41.0
20	37.24700107110958	39.0	35.0	41.0	33.0	41.0
21	37.11339260614127	39.0	35.0	41.0	32.0	41.0
22	37.065044997277745	39.0	35.0	41.0	32.0	41.0
23	36.976209624328774	38.0	35.0	41.0	32.0	41.0
24	36.91834034951622	38.0	35.0	41.0	32.0	41.0
25	36.86041769714999	38.0	35.0	41.0	32.0	41.0
26	36.82015456360291	38.0	35.0	41.0	32.0	41.0
27	36.72787322475153	38.0	35.0	41.0	32.0	41.0
28	36.69926152154496	38.0	35.0	41.0	32.0	41.0
29	36.61004005095777	38.0	35.0	41.0	31.0	41.0
30	36.539589241931985	38.0	35.0	41.0	31.0	41.0
31	36.360977716650595	38.0	35.0	41.0	31.0	41.0
32	36.02177893152371	38.0	35.0	41.0	30.0	41.0
33	35.79178128013608	38.0	35.0	41.0	29.0	41.0
34	35.47404249564973	38.0	35.0	41.0	27.0	41.0
35	35.27183008501265	38.0	35.0	41.0	25.0	41.0
36	35.16470622773711	38.0	35.0	41.0	24.0	41.0
37	34.894520616190476	38.0	34.0	40.0	23.0	41.0
38	34.99387848421981	38.0	34.0	40.0	24.0	41.0
39	34.909699858015706	38.0	34.0	40.0	23.0	41.0
40	34.809968347110676	38.0	34.0	40.0	23.0	41.0
41	34.728923428120005	38.0	34.0	40.0	23.0	41.0
42	34.72709658134561	38.0	34.0	40.0	23.0	41.0
43	34.63231228004	38.0	34.0	40.0	23.0	41.0
44	34.47707611995004	38.0	34.0	40.0	22.0	41.0
45	34.47726561026557	38.0	34.0	40.0	23.0	41.0
46	34.28069476223858	37.0	34.0	40.0	22.0	41.0
47	34.190573435059086	37.0	34.0	40.0	21.0	41.0
48	34.05452383663622	37.0	33.0	40.0	20.0	41.0
49	34.1171116871933	37.0	34.0	40.0	21.0	41.0
50	33.99671194269386	37.0	33.0	40.0	20.0	41.0
51	33.42567620464242	36.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	3.0
12	7.0
13	13.0
14	24.0
15	58.0
16	155.0
17	397.0
18	986.0
19	1938.0
20	3433.0
21	5619.0
22	8626.0
23	12930.0
24	19611.0
25	31246.0
26	43916.0
27	50373.0
28	49459.0
29	47973.0
30	50917.0
31	58620.0
32	71039.0
33	91799.0
34	155307.0
35	257560.0
36	149384.0
37	188084.0
38	306045.0
39	642471.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.246123899977583	22.39335164776508	28.047191095200645	19.313333357056692
2	35.32499813178562	23.818265443015903	28.56833394420978	12.288402480988694
3	26.788859748698478	23.791843553948695	36.78972268581616	12.629574011536668
4	24.480147108537917	25.71881772277122	36.14905859787842	13.65197657081244
5	22.57950586619315	29.92287833120416	34.55004501507026	12.947570787532428
6	20.379505510342792	37.6430073625439	31.71729824174338	10.260188885369924
7	82.86491564567268	3.342146560528367	12.33973389510243	1.4532038986965201
8	83.98971414540756	2.690095261140785	11.811740926705502	1.50844966674614
9	80.40510004732809	4.705186874815403	12.738775590088855	2.150937487767644
10	41.339447435564395	34.4408879178128	17.066805566922998	7.152859079699804
11	29.90704299028173	23.125513758954085	32.595314518338746	14.372128732425441
12	29.572098840995388	22.073130807033024	32.91126515477711	15.443505197194476
13	22.183978193490073	29.31633139632122	32.32068700470078	16.179003405487926
14	15.172569631018765	33.49721725020194	34.084592747058004	17.245620371721284
15	14.4540187960159	28.19317870449119	42.533102979535045	14.819699519957869
16	18.39986548856475	25.668153528078374	41.0102413732977	14.921739610059179
17	18.76572413768562	24.5727126828626	33.584044737506986	23.077518441944793
18	19.235446609991566	26.706435909571308	36.320889839404735	17.737227641032394
19	22.7992434621393	27.22637776362284	32.38198222883313	17.592396545404725
20	24.75174989413452	27.171443364636303	32.4559991032571	15.620807637972081
21	22.665577171487847	26.114478839358473	34.811595028058804	16.40834896109488
22	21.255698053854395	22.90034944505137	34.91221171672888	20.931740784365356
23	18.08097908667447	26.712485365654036	35.11504642067918	20.091489126992318
24	17.76356056751015	26.21700822370177	39.15123462281641	16.868196585971667
25	17.577406349082082	28.369902888437355	35.91922374802948	18.13346701445108
26	17.4294615628236	32.884798784415175	31.8351291914724	17.850610461288817
27	16.873178490980973	31.922534935608876	34.72298828896472	16.481298284445426
28	15.10522495080369	29.377315251390485	39.42305981488665	16.09439998291918
29	16.297279168164202	26.47032919716601	38.73769202574933	18.494699608920456
30	17.27324325574609	28.579765636954345	36.48876224570044	17.65822886159912
31	22.298117195756838	27.6115857759495	32.26290580285178	17.827391225441875
32	22.27600999227805	28.663835283986376	32.36641377567906	16.693740948056522
33	21.360718390702342	29.32638416892928	31.380085546426017	17.93281189394236
34	18.03494094663312	29.767638612610625	32.644021536063654	19.5533989046926
35	18.167717611390056	29.00073661024066	32.64246469074825	20.189081087621034
36	22.20470647683236	28.145227868776622	31.79402847514563	17.856037179245384
37	18.909843532597673	30.615318646202898	32.25098481586523	18.2238530053342
38	18.260060779241112	31.578827971261525	29.850729671158682	20.310381578338678
39	18.43634015024002	30.30083589249049	31.618727692630692	19.644096264638794
40	20.331688118512403	28.877301017376173	30.605265873594835	20.18574499051659
41	17.570956561346822	27.568216513591704	32.066076073689494	22.79475085137198
42	19.994164054131957	28.685542155812637	29.552037777073988	21.76825601298142
43	20.600533063835996	28.916578000619182	29.516719629061587	20.966169306483238
44	18.59802965656882	31.402771006736245	30.80578755021938	19.19341178647555
45	18.426109452453055	33.54903795855766	28.294729500350513	19.730123088638766
46	20.40583843682055	31.52384909098026	29.326028318571474	18.744284153627717
47	19.386149236523057	30.366445802211256	30.50500503528256	19.742399925983126
48	20.24099075856621	28.677224153698887	31.600801730856144	19.480983356878763
49	19.180289804531398	27.95235697484494	31.991747830202442	20.87560539042122
50	17.870182230968233	31.189794567588436	31.69145460950761	19.24856859193572
51	17.293170875783314	31.992192643149703	29.891963831369633	20.82267264969735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2008.0
1	2653.5
2	3299.0
3	35029.0
4	66759.0
5	44056.5
6	21354.0
7	21451.0
8	21548.0
9	22292.0
10	23036.0
11	23156.0
12	23276.0
13	22723.5
14	22171.0
15	21125.5
16	20080.0
17	18745.5
18	17411.0
19	17474.5
20	17538.0
21	17495.5
22	17453.0
23	17946.0
24	18439.0
25	21047.0
26	28191.5
27	32728.0
28	38080.0
29	43432.0
30	49463.5
31	55495.0
32	63840.5
33	72186.0
34	78786.5
35	85387.0
36	91151.5
37	96916.0
38	106425.0
39	115934.0
40	132099.5
41	148265.0
42	167662.0
43	187059.0
44	189752.0
45	192445.0
46	187003.0
47	181561.0
48	172554.5
49	163548.0
50	148329.5
51	133111.0
52	123934.5
53	114758.0
54	102331.5
55	89905.0
56	79406.5
57	68908.0
58	63245.0
59	57582.0
60	50333.5
61	43085.0
62	36124.0
63	29163.0
64	23832.0
65	18501.0
66	13917.5
67	9334.0
68	7333.0
69	5332.0
70	3887.5
71	2443.0
72	1937.5
73	1432.0
74	1159.0
75	622.0
76	358.0
77	264.0
78	170.0
79	138.5
80	107.0
81	76.5
82	46.0
83	30.0
84	14.0
85	11.5
86	9.0
87	5.0
88	1.0
89	2.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2248136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.31816278200911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.52505072358468	18.85266803179646
2	6.5905594574195066	3.205405954200744
3	2.834577934713187	2.067951829039395
4	1.8172433104556407	1.7676807455270955
5	1.3288744684801155	1.6157892820677644
6	0.9940291651287885	1.4503777828599895
7	0.8135368923642194	1.3848605804378042
8	0.6494304305446318	1.2634363940459694
9	0.5251946316294149	1.1494591689781282
>10	5.074277703544524	28.2880413855637
>50	1.517551532897443	25.927048036873064
>100	0.32577999618042763	10.223767316915984
>500	0.0029666689962067885	0.4912100485478928
>1k	7.416672490516971E-4	0.24840060193228766
>5k	0.0	0.0
>10k+	1.8541681226292428E-4	2.0639028412137597
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45773	2.0360423034905364	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029313173224395677	0.0
2	0.0	0.0	0.0	0.09323279374557411	0.0
3	0.0	0.0	0.0	0.13740271940843438	0.0
4	0.0	0.0	0.0	0.21173096289548318	0.0
5	0.0	0.0	0.0	0.4063811086162047	0.0
6	0.0	0.0	0.0	0.6745588345189081	0.0
7	0.0	0.0	0.0	0.8752139550276318	0.0
8	0.0	0.0	0.0	1.1736389613439757	0.0
9	0.0	0.0	4.448129472594185E-5	1.3208720468868431	0.0
10	0.0	0.0	8.89625894518837E-5	1.5078714099147026	0.0
11	0.0	0.0	8.89625894518837E-5	1.7099054505599305	0.0
12	0.0	0.0	8.89625894518837E-5	1.868748154026269	0.0
13	0.0	0.0	8.89625894518837E-5	1.942765028450236	0.0
14	0.0	0.0	8.89625894518837E-5	1.98404366995591	0.0
15	0.0	0.0	8.89625894518837E-5	2.0311493610706823	0.0
16	0.0	0.0	8.89625894518837E-5	2.1091695520199845	0.0
17	0.0	0.0	8.89625894518837E-5	2.211609973773829	0.0
18	0.0	0.0	8.89625894518837E-5	2.3424294615628236	0.0
19	0.0	0.0	8.89625894518837E-5	2.425298113637253	0.0
20	0.0	0.0	8.89625894518837E-5	2.5124814513000993	0.0
21	0.0	0.0	8.89625894518837E-5	2.620393072305234	0.0
22	0.0	0.0	8.89625894518837E-5	2.7456523982534864	0.0
23	0.0	0.0	8.89625894518837E-5	2.8810089781045276	0.0
24	0.0	0.0	8.89625894518837E-5	2.9890540429938404	0.0
25	0.0	0.0	8.89625894518837E-5	3.076548749719768	0.0
26	0.0	0.0	8.89625894518837E-5	3.1626200550144654	0.0
27	0.0	0.0	8.89625894518837E-5	3.2496699487931333	0.0
28	0.0	0.0	8.89625894518837E-5	3.3515321137155403	0.0
29	0.0	0.0	8.89625894518837E-5	3.4638918641932697	0.0
30	0.0	0.0	8.89625894518837E-5	3.5999156634651994	0.0
31	0.0	0.0	8.89625894518837E-5	3.7239739944558514	0.0
32	0.0	0.0	8.89625894518837E-5	3.838379884490974	0.0
33	0.0	0.0	8.89625894518837E-5	3.9509175601476065	0.0
34	0.0	0.0	8.89625894518837E-5	4.065012081119647	0.0
35	0.0	0.0	8.89625894518837E-5	4.205839860221979	0.0
36	0.0	0.0	8.89625894518837E-5	4.330387485454617	0.0
37	0.0	0.0	8.89625894518837E-5	4.458760502033685	0.0
38	0.0	0.0	8.89625894518837E-5	4.5952735955476	0.0
39	0.0	0.0	8.89625894518837E-5	4.747399623510321	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	30	2.166431E-6	45.000004	1
TCACGAC	325	0.0	44.307693	25
CGTTTTT	20345	0.0	44.08208	1
CGCACGG	90	0.0	42.5	2
CGGTCTA	335	0.0	42.313435	31
TAGTACG	75	0.0	42.0	1
TCGTCCC	270	0.0	41.666668	38
CGACGGT	350	0.0	41.142857	28
GACGGTC	350	0.0	41.142857	29
CACGACG	350	0.0	40.5	26
ACGGTCT	350	0.0	40.5	30
TGCGTAG	225	0.0	40.0	1
CTATGCG	125	0.0	39.6	1
CTCGTCC	290	0.0	39.56897	37
GTTTTTT	23845	0.0	38.64961	2
GGCGATA	445	0.0	38.426968	8
TAGGGAT	3685	0.0	38.4057	5
AGGGATT	4585	0.0	38.276993	6
GTTAGCG	100	0.0	38.25	1
AAGGGAT	3525	0.0	38.234043	5
>>END_MODULE