FastQCFastQC Report
Thu 26 May 2016
SRR1547716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547716_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1454366
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72450.4981552098990213No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29010.19946835940884206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT207.0339034E-445.0000045
GATCGTA207.0339034E-445.00000416
TAGGTCG406.8175723E-945.0000041
CCGGAAT207.0339034E-445.00000443
CGTTCGG207.0339034E-445.00000445
CGCTAAG207.0339034E-445.00000443
AGGTCGT302.1659016E-645.00000414
AATATCG253.8913182E-545.030
CGAAGGC253.8913182E-545.023
TCGTTAG551.8189894E-1244.9999961
CGGTCTA2600.044.99999631
CGTAAGG1700.043.6764682
CGACAGG1050.042.8571432
CTCACGA3050.042.78688424
CGTTTTT53750.042.6139531
GTTAGCG850.042.352941
TCGTAAG750.042.01
TCACGAC3000.041.2525
CACGACG2900.041.1206926
CGAGGGC2850.041.052634