##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547716_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1454366
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70058912268301	31.0	31.0	33.0	30.0	34.0
2	32.08864274879913	33.0	31.0	34.0	30.0	34.0
3	32.14648169717939	33.0	31.0	34.0	30.0	34.0
4	35.85252199240081	37.0	35.0	37.0	35.0	37.0
5	35.789505530244796	37.0	35.0	37.0	35.0	37.0
6	35.849599756870006	37.0	35.0	37.0	35.0	37.0
7	36.14185218851376	37.0	35.0	37.0	35.0	37.0
8	36.0209369580972	37.0	35.0	37.0	35.0	37.0
9	37.797007080748585	39.0	38.0	39.0	35.0	39.0
10	37.47702847838852	39.0	37.0	39.0	35.0	39.0
11	37.18965102319498	39.0	37.0	39.0	34.0	39.0
12	36.49741055552729	38.0	35.0	39.0	33.0	39.0
13	36.24801459880113	38.0	35.0	39.0	32.0	39.0
14	37.212844634706805	39.0	35.0	41.0	33.0	41.0
15	37.425696145261924	39.0	35.0	41.0	33.0	41.0
16	37.52140520336697	39.0	35.0	41.0	33.0	41.0
17	37.42586941663928	39.0	35.0	41.0	33.0	41.0
18	37.29309609823112	39.0	35.0	41.0	33.0	41.0
19	37.272714708677185	39.0	35.0	41.0	33.0	41.0
20	37.17961365983528	39.0	35.0	41.0	33.0	41.0
21	37.03082580313346	39.0	35.0	41.0	32.0	41.0
22	37.00402924710836	38.0	35.0	41.0	32.0	41.0
23	36.93464437424967	38.0	35.0	41.0	32.0	41.0
24	36.93365356450852	38.0	35.0	41.0	32.0	41.0
25	36.86606947632164	38.0	35.0	41.0	32.0	41.0
26	36.81821701002361	38.0	35.0	41.0	32.0	41.0
27	36.74259780550425	38.0	35.0	41.0	32.0	41.0
28	36.72662658505493	38.0	35.0	41.0	32.0	41.0
29	36.678708110613144	38.0	35.0	41.0	32.0	41.0
30	36.684221165786326	38.0	35.0	41.0	32.0	41.0
31	36.523779433787645	38.0	35.0	41.0	31.0	41.0
32	36.3049424972806	38.0	35.0	41.0	31.0	41.0
33	36.200439916774734	38.0	35.0	41.0	30.0	41.0
34	35.98710503408358	38.0	35.0	41.0	30.0	41.0
35	35.859414342744536	38.0	35.0	41.0	30.0	41.0
36	35.79288432210324	38.0	35.0	41.0	30.0	41.0
37	35.559290439958026	38.0	35.0	40.0	29.0	41.0
38	35.65366902141552	38.0	35.0	40.0	29.0	41.0
39	35.599557470402914	38.0	35.0	40.0	29.0	41.0
40	35.47625081994491	38.0	35.0	40.0	28.0	41.0
41	35.435875838681596	38.0	35.0	40.0	28.0	41.0
42	35.44912009769205	38.0	35.0	40.0	29.0	41.0
43	35.37399939217501	38.0	35.0	40.0	28.0	41.0
44	35.239981545223145	37.0	34.0	40.0	27.0	41.0
45	35.218618284530855	37.0	34.0	40.0	27.0	41.0
46	35.04585503236462	37.0	34.0	40.0	27.0	41.0
47	34.92690354422477	37.0	34.0	40.0	26.0	41.0
48	34.808725589019545	37.0	34.0	40.0	26.0	41.0
49	34.89120551498041	36.0	34.0	40.0	27.0	41.0
50	34.76295100408013	36.0	34.0	40.0	26.0	41.0
51	34.18891874535021	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	3.0
11	4.0
12	11.0
13	17.0
14	23.0
15	41.0
16	111.0
17	247.0
18	515.0
19	1045.0
20	1741.0
21	2837.0
22	4421.0
23	6618.0
24	9882.0
25	14712.0
26	19940.0
27	23111.0
28	24073.0
29	26410.0
30	30028.0
31	36178.0
32	46508.0
33	62708.0
34	120646.0
35	207257.0
36	86398.0
37	117611.0
38	195427.0
39	415735.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.86421712278752	22.965814657383355	28.68370135165426	15.48626686817486
2	32.286920898865894	24.561286498721778	29.483362509849652	13.66843009256267
3	28.937901463593068	24.365393580432986	32.260517641364004	14.436187314609942
4	27.198655634138863	26.187080831097536	30.351782151122897	16.262481383640708
5	24.14591650244849	30.823740378969255	30.130517352578373	14.899825766003879
6	22.65832672105921	38.875358747385455	27.446323690185277	11.019990841370054
7	85.239616437678	4.574639396135498	7.924071382306792	2.2616727838797113
8	85.6714197114069	4.113682525581594	7.886804284478598	2.328093478532914
9	81.52184525765867	5.855678694358916	9.260323742441724	3.3621523055406963
10	46.90215530341056	26.01882882300604	17.02439413462636	10.054621738957044
11	39.85874257236486	22.104339622900977	23.644735919294043	14.392181885440117
12	37.55767117768155	21.46564207359083	25.756308934614808	15.220377814112815
13	25.474261637029468	32.212661737141815	25.521911265802416	16.791165360026294
14	16.858067364061043	35.839534202532235	29.634493655654765	17.667904777751957
15	16.735952298114782	25.931918100395634	42.49576791536656	14.836361686123025
16	20.94761566208231	22.655232589320708	40.70089647310237	15.696255275494615
17	21.036314105252735	21.83659408979583	29.550058238435167	27.577033566516267
18	23.260582274338095	25.367204678877258	32.881131709624675	18.491081337159972
19	27.287835386690833	26.696787466153637	27.560462772094507	18.454914375061023
20	29.62988683728855	26.56202083932105	26.610495569890936	17.197596753499464
21	24.64249026723672	26.48439251192616	30.48427974801391	18.388837472823212
22	22.828435208193813	23.446711488029838	30.43284840267168	23.292004901104672
23	20.020751310192892	27.260675785875083	30.46069558831821	22.257877315613815
24	21.9251550160001	24.45388574815418	35.425264341988196	18.19569489385753
25	20.087928348159952	25.37339294235426	33.4174478776319	21.12123083185388
26	18.69770057880891	32.454760356058934	28.198885287472343	20.64865377765982
27	18.260740418849174	32.13881512631621	32.117775030494386	17.482669424340227
28	16.224732976430968	29.327211994779855	35.91833142413945	18.52972360464972
29	18.185381121395853	26.407107976946655	35.87315710075731	19.53435380090019
30	20.179170855204262	28.27665113183339	33.328199366596856	18.215978646365496
31	26.536098891200705	26.040831537590954	28.96794892069809	18.455120650510256
32	27.330190612266787	27.632865454775484	28.221781862337263	16.815162070620463
33	26.20296404068852	28.398078612948872	27.35480614920866	18.044151197153948
34	20.560780436286326	29.087107371872005	29.274336721292986	21.07777547054868
35	20.54950404506156	28.480863826574605	30.296225296795992	20.673406831567846
36	26.524547466043625	27.052681374564585	28.583795275742148	17.838975883649645
37	20.927881977438968	31.44868623166383	29.495395244388277	18.128036546508923
38	21.48585706761572	30.626059740120436	26.081949110471502	21.80613408179234
39	20.796278240827963	30.174935332646662	27.990615842229534	21.038170584295838
40	22.669053044419353	27.87138863257254	28.041428361224064	21.418129961784036
41	18.78137965271465	26.65443224057768	28.913973511481977	25.650214595225684
42	21.990544333407133	27.550974101429766	26.36956584518615	24.088915719976953
43	22.11788504406731	27.396198756021523	27.015276759770234	23.47063944014093
44	19.92600212051162	30.23111101332127	28.43397054111551	21.408916325051603
45	19.82967148571955	33.54932664817522	25.283594363454593	21.337407502650642
46	22.742693379795732	31.258637784436655	26.315177885071574	19.683490950696044
47	21.51686714348383	29.52647407873946	27.102875067211418	21.853783710565292
48	22.82644121218455	26.49401869955706	30.389393041366482	20.29014704689191
49	22.178117475243507	25.634056351702387	30.29808177583909	21.889744397215004
50	20.063725362116553	30.36663398346771	28.708798197977675	20.860842456438064
51	18.965858662812522	32.00006050746511	27.592779259141096	21.44130157058127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	969.0
1	2205.5
2	3442.0
3	9086.5
4	14731.0
5	10704.0
6	6677.0
7	6708.0
8	6739.0
9	7178.0
10	7617.0
11	7422.5
12	7228.0
13	7216.5
14	7205.0
15	6707.0
16	6209.0
17	5929.5
18	5650.0
19	5860.0
20	6070.0
21	6708.0
22	7346.0
23	7461.0
24	7576.0
25	9390.0
26	13406.5
27	15609.0
28	18324.0
29	21039.0
30	25161.0
31	29283.0
32	33233.5
33	37184.0
34	40029.5
35	42875.0
36	47294.5
37	51714.0
38	57969.5
39	64225.0
40	78292.5
41	92360.0
42	109163.0
43	125966.0
44	130878.5
45	135791.0
46	131442.5
47	127094.0
48	120928.0
49	114762.0
50	103379.0
51	91996.0
52	90223.5
53	88451.0
54	78810.5
55	69170.0
56	64567.0
57	59964.0
58	59037.5
59	58111.0
60	52976.5
61	47842.0
62	40385.0
63	32928.0
64	26956.5
65	20985.0
66	16475.0
67	11965.0
68	9522.0
69	7079.0
70	5285.5
71	3492.0
72	3030.5
73	2569.0
74	2164.5
75	1212.0
76	664.0
77	566.5
78	469.0
79	293.5
80	118.0
81	111.5
82	105.0
83	81.5
84	58.0
85	45.5
86	33.0
87	21.0
88	9.0
89	10.0
90	11.0
91	9.5
92	8.0
93	6.5
94	5.0
95	6.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1454366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.156227865173292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.84514012966656	14.859990138512646
2	5.438186422933521	1.974739037361453
3	1.6177245744779367	0.8811532799193574
4	0.8618851484256016	0.6259433259369569
5	0.5649604286250893	0.5128775138461547
6	0.42836422546483216	0.4666487092096776
7	0.35176020156243815	0.44706468614268385
8	0.3235181016371429	0.46990946974658065
9	0.2980028973262297	0.486954765750318
>10	4.716612917048011	21.503459374922535
>50	2.6058226012152854	34.75411294926677
>100	0.9453540136755945	22.09533967852742
>500	0.0019059556727330535	0.2196031110316569
>1k	3.8119113454661074E-4	0.20077800980234378
>5k	3.8119113454661074E-4	0.5014259500234335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7245	0.4981552098990213	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2901	0.19946835940884206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3751696615569946E-4	0.0	0.0	0.009832463080132511	0.0
2	1.3751696615569946E-4	0.0	0.0	0.03369165670814637	0.0
3	1.3751696615569946E-4	0.0	0.0	0.04579314972984792	0.0
4	1.3751696615569946E-4	0.0	0.0	0.07006489425632888	0.0
5	1.3751696615569946E-4	0.0	0.0	0.12410906195551877	0.0
6	1.3751696615569946E-4	0.0	0.0	0.17801571268855296	0.0
7	1.3751696615569946E-4	0.0	0.0	0.2163829462459931	0.0
8	1.3751696615569946E-4	0.0	0.0	0.30439380458564075	0.0
9	1.3751696615569946E-4	0.0	0.0	0.34434248325387146	0.0
10	1.3751696615569946E-4	0.0	0.0	0.4210081918856739	0.0
11	1.3751696615569946E-4	0.0	0.0	0.5585939165244512	0.0
12	1.3751696615569946E-4	0.0	0.0	0.657537373673477	0.0
13	1.3751696615569946E-4	0.0	0.0	0.6989987389694203	0.0
14	1.3751696615569946E-4	0.0	0.0	0.7166696691204277	0.0
15	1.3751696615569946E-4	0.0	0.0	0.744448096283879	0.0
16	1.3751696615569946E-4	0.0	0.0	0.8011050863400272	0.0
17	1.3751696615569946E-4	0.0	0.0	0.8695197770024877	0.0
18	1.3751696615569946E-4	0.0	0.0	0.951754922763596	0.0
19	1.3751696615569946E-4	0.0	0.0	0.9995420685027016	0.0
20	1.3751696615569946E-4	0.0	0.0	1.055992783109616	0.0
21	1.3751696615569946E-4	0.0	0.0	1.1267452621967236	0.0
22	1.3751696615569946E-4	0.0	0.0	1.1996292542592442	0.0
23	1.3751696615569946E-4	0.0	0.0	1.2749885517125676	0.0
24	1.3751696615569946E-4	0.0	0.0	1.3337082962610511	0.0
25	1.3751696615569946E-4	0.0	0.0	1.3920842483941456	0.0
26	1.3751696615569946E-4	0.0	0.0	1.4407652544132632	0.0
27	1.3751696615569946E-4	0.0	0.0	1.4981785877832678	0.0
28	1.3751696615569946E-4	0.0	0.0	1.5562795059840508	0.0
29	1.3751696615569946E-4	0.0	0.0	1.6267569511388467	0.0
30	1.3751696615569946E-4	0.0	0.0	1.7047290709491283	0.0
31	1.3751696615569946E-4	0.0	0.0	1.7789194741901282	0.0
32	1.3751696615569946E-4	0.0	0.0	1.844858859461786	0.0
33	1.3751696615569946E-4	0.0	0.0	1.9170552666935283	0.0
34	1.3751696615569946E-4	0.0	0.0	1.9915894623499175	0.0
35	1.3751696615569946E-4	0.0	0.0	2.0877825801758294	0.0
36	1.3751696615569946E-4	0.0	0.0	2.165342149087644	0.0
37	1.3751696615569946E-4	0.0	0.0	2.25211535473189	0.0
38	1.3751696615569946E-4	0.0	0.0	2.3492023328378138	0.0
39	1.3751696615569946E-4	0.0	0.0	2.4846565445011777	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	7.0339034E-4	45.000004	5
GATCGTA	20	7.0339034E-4	45.000004	16
TAGGTCG	40	6.8175723E-9	45.000004	1
CCGGAAT	20	7.0339034E-4	45.000004	43
CGTTCGG	20	7.0339034E-4	45.000004	45
CGCTAAG	20	7.0339034E-4	45.000004	43
AGGTCGT	30	2.1659016E-6	45.000004	14
AATATCG	25	3.8913182E-5	45.0	30
CGAAGGC	25	3.8913182E-5	45.0	23
TCGTTAG	55	1.8189894E-12	44.999996	1
CGGTCTA	260	0.0	44.999996	31
CGTAAGG	170	0.0	43.676468	2
CGACAGG	105	0.0	42.857143	2
CTCACGA	305	0.0	42.786884	24
CGTTTTT	5375	0.0	42.613953	1
GTTAGCG	85	0.0	42.35294	1
TCGTAAG	75	0.0	42.0	1
TCACGAC	300	0.0	41.25	25
CACGACG	290	0.0	41.12069	26
CGAGGGC	285	0.0	41.05263	4
>>END_MODULE