FastQCFastQC Report
Thu 26 May 2016
SRR1547715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547715_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1618549
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230201.4222615441361366No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17330.10707121007766833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCG302.1660526E-645.0000041
CCGCTCG600.045.00000419
TCGCGAG302.1660526E-645.0000041
TCACCGT253.891514E-545.024
ATAGCGC253.891514E-545.018
CGACATA253.891514E-545.017
CGTCGGA207.034142E-445.017
CGCGTCA253.891514E-545.039
ATACGCG406.8175723E-945.01
TACCGTA207.034142E-445.040
CGTTTTT118800.043.8446961
CGGTCTA3650.043.7671231
CGTATGG2000.042.752
CGACGGT3750.042.628
GACCGAT2800.042.5892839
CGTAAGG2300.042.065222
TTTCGCG750.042.01
CACGACG3850.041.49350426
TAGGGTA11250.041.45
TCGTGCG501.0822987E-940.51
Produced by FastQC (version 0.11.5)