##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547715_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1618549
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.625989698180284	31.0	31.0	33.0	30.0	34.0
2	32.01372525638705	33.0	31.0	34.0	30.0	34.0
3	32.019159135744424	33.0	31.0	34.0	30.0	34.0
4	35.7743923724274	37.0	35.0	37.0	35.0	37.0
5	35.724593447587935	37.0	35.0	37.0	33.0	37.0
6	35.78734471430893	37.0	35.0	37.0	35.0	37.0
7	36.14153417659892	37.0	35.0	37.0	35.0	37.0
8	36.04688211478305	37.0	35.0	37.0	35.0	37.0
9	37.87145832470935	39.0	38.0	39.0	35.0	39.0
10	37.37715200466591	39.0	37.0	39.0	34.0	39.0
11	37.13349796638841	39.0	37.0	39.0	34.0	39.0
12	36.61531655822592	39.0	35.0	39.0	33.0	39.0
13	36.427214746047234	39.0	35.0	39.0	33.0	39.0
14	37.44590556109206	40.0	35.0	41.0	33.0	41.0
15	37.65550749467579	40.0	35.0	41.0	33.0	41.0
16	37.75222745805039	40.0	35.0	41.0	33.0	41.0
17	37.66786609487881	40.0	35.0	41.0	33.0	41.0
18	37.511835600899325	39.0	36.0	41.0	33.0	41.0
19	37.40940929190281	39.0	36.0	41.0	33.0	41.0
20	37.25085307889968	39.0	35.0	41.0	33.0	41.0
21	37.114969024725234	39.0	35.0	41.0	33.0	41.0
22	37.08503665937825	39.0	35.0	41.0	33.0	41.0
23	37.004211179272296	38.0	35.0	41.0	32.0	41.0
24	36.97234004036949	38.0	35.0	41.0	32.0	41.0
25	36.90655024963718	38.0	35.0	41.0	32.0	41.0
26	36.874198433288086	38.0	35.0	41.0	32.0	41.0
27	36.78606640886374	38.0	35.0	41.0	32.0	41.0
28	36.762789387284535	38.0	35.0	41.0	32.0	41.0
29	36.7114674934154	38.0	35.0	41.0	32.0	41.0
30	36.6617754544348	38.0	35.0	41.0	32.0	41.0
31	36.46660187612485	38.0	35.0	41.0	31.0	41.0
32	36.1732582702161	38.0	35.0	41.0	30.0	41.0
33	35.97520989478848	38.0	35.0	41.0	30.0	41.0
34	35.71754824846205	38.0	35.0	41.0	29.0	41.0
35	35.53383802405735	38.0	35.0	41.0	27.0	41.0
36	35.42121245634207	38.0	35.0	41.0	27.0	41.0
37	35.150021408063644	38.0	34.0	40.0	25.0	41.0
38	35.25762951878504	38.0	35.0	40.0	26.0	41.0
39	35.18123949290383	38.0	35.0	40.0	25.0	41.0
40	35.08352048655926	38.0	35.0	40.0	24.0	41.0
41	35.01402923235565	38.0	34.0	40.0	24.0	41.0
42	35.0238133043856	38.0	34.0	40.0	24.0	41.0
43	34.93291707572647	38.0	34.0	40.0	24.0	41.0
44	34.81017503949525	38.0	34.0	40.0	23.0	41.0
45	34.78975860477502	38.0	34.0	40.0	24.0	41.0
46	34.62852097773994	37.0	34.0	40.0	23.0	41.0
47	34.53048131381873	37.0	34.0	40.0	23.0	41.0
48	34.38776892142283	37.0	34.0	40.0	23.0	41.0
49	34.453312812895994	37.0	34.0	40.0	24.0	41.0
50	34.32316661404752	37.0	34.0	40.0	23.0	41.0
51	33.750031664163394	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	2.0
13	2.0
14	8.0
15	30.0
16	80.0
17	223.0
18	552.0
19	1050.0
20	2031.0
21	3465.0
22	5239.0
23	8175.0
24	12454.0
25	19930.0
26	28102.0
27	31967.0
28	31903.0
29	31289.0
30	34859.0
31	41221.0
32	51225.0
33	68224.0
34	120141.0
35	209233.0
36	104319.0
37	133192.0
38	218561.0
39	460967.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.019233276224572	23.188423705429987	29.87292939540292	16.919413622942525
2	33.22197845106945	23.97888479125439	30.170603423189533	12.62853333448663
3	27.48727409550159	23.769932204709278	36.144966880829685	12.597826818959451
4	24.940981088617026	26.519431910927626	34.86085376469912	13.678733235756225
5	22.881729252558927	30.250736925480787	33.4854860742554	13.38204774770489
6	21.246066693068915	39.22105540209163	29.831596077721468	9.701281827117993
7	85.8552938465255	3.0544642145526644	9.882678868542133	1.2075630703797045
8	87.3353849651756	2.2677101527355674	9.360235618445904	1.0366692636429296
9	83.40019363022066	3.8314564464838567	10.890371561194625	1.8779783621008694
10	40.89063723124848	25.632649984646743	23.633266586306622	9.843446197798151
11	35.68813795566276	21.364691461302684	27.452860555967103	15.494310027067455
12	33.58928274645995	22.140571586031687	28.86152967874312	15.408615988765245
13	24.138286823568517	31.068259286558515	29.319717846046057	15.473736043826907
14	16.69322337476345	35.055905011216836	31.77852508635821	16.472346527661504
15	15.774746393220102	27.408129132945618	42.2452455872513	14.571878886582985
16	19.851422477787203	24.17609846844303	40.97046181487246	15.002017238897308
17	19.732797709553434	23.415293574677072	31.51161935783223	25.340289357937262
18	21.089074226359536	26.00619443711621	35.399113650559855	17.505617685964403
19	24.445475546307215	26.87314378495801	30.73987874324472	17.94150192549005
20	26.739134867093924	26.72832271373928	29.680164147023042	16.852378272143753
21	22.96544621139057	26.77045921995565	33.42969536294545	16.834399205708323
22	21.916543768523535	22.316840577579054	33.41770931865516	22.348906335242244
23	18.104610981811486	27.418385232699165	33.019451372803665	21.457552412685683
24	18.347421054289985	26.47686291857707	38.20069704408084	16.975018983052102
25	18.09423131459103	27.479489345086243	35.74436115310689	18.681918187215835
26	17.54880451564951	32.98713848020666	30.41415490046949	19.04990210367434
27	17.256691023873852	33.05071394193194	33.38366648152141	16.308928552672793
28	15.242603096971424	29.26485389073794	38.4903391865183	17.002203825772344
29	16.82914758836464	26.94802566990558	38.052477867522086	18.1703488742077
30	18.75958034016888	29.12139206165522	34.878338561266915	17.240689036908986
31	24.65238926964831	27.390829687578194	30.22725910676785	17.729521936005643
32	24.859117641789037	28.479582638523766	30.73407107229994	15.92722864738726
33	23.645129063130003	28.31672071713615	29.990133137767224	18.048017081966623
34	19.084933480543377	29.036501211887934	31.083458084988468	20.795107222580224
35	18.800542955449604	28.68390144505974	32.43256768871378	20.082987910776875
36	24.856090238849735	26.909843322630334	30.8185912196665	17.41547521885343
37	19.487207369069456	30.960323104212478	31.190035025198497	18.36243450151957
38	19.383534264331818	32.05599583330502	27.77691623793904	20.783553664424122
39	19.084130292008457	31.162541263810983	30.234796722249374	19.518531721931186
40	21.928838731481097	28.602717619299757	28.691439060541263	20.777004588677883
41	18.767982927918773	27.0211776103164	30.540070149250965	23.670769312513862
42	20.67858310128393	27.725635739171317	28.58498568779815	23.010795471746608
43	20.899151029718592	27.736015406391772	28.703363321098095	22.661470242791538
44	19.659522201675696	30.272114097256246	30.30763974399292	19.760723957075133
45	18.36632687672724	33.83555270801193	27.608184861873198	20.189935553387635
46	21.273004400855335	31.28746797285717	28.59802205555717	18.841505570730327
47	20.33296489633616	29.835735587862956	28.937585454626337	20.893714061174546
48	20.984350798153162	28.49144511534714	30.99832010028736	19.525883986212342
49	20.72485911764179	26.89995792527752	31.605159930283232	20.77002302679746
50	19.002822898781563	31.6362989319446	29.860140162577714	19.50073800669612
51	18.497864445253125	32.78467318567433	28.414771502129376	20.30269086694317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1365.0
1	1855.0
2	2345.0
3	18485.5
4	34626.0
5	23326.0
6	12026.0
7	12155.0
8	12284.0
9	12893.0
10	13502.0
11	13606.5
12	13711.0
13	13521.5
14	13332.0
15	12599.0
16	11866.0
17	11541.0
18	11216.0
19	10175.5
20	9135.0
21	8913.5
22	8692.0
23	9826.0
24	10960.0
25	11934.0
26	15782.0
27	18656.0
28	22000.0
29	25344.0
30	29975.5
31	34607.0
32	38917.0
33	43227.0
34	49515.5
35	55804.0
36	59220.0
37	62636.0
38	69876.0
39	77116.0
40	91739.5
41	106363.0
42	126291.0
43	146219.0
44	148188.5
45	150158.0
46	146537.5
47	142917.0
48	135474.5
49	128032.0
50	120714.0
51	113396.0
52	104036.0
53	94676.0
54	84748.0
55	74820.0
56	66029.5
57	57239.0
58	50481.5
59	43724.0
60	37490.0
61	31256.0
62	25879.5
63	20503.0
64	16010.0
65	11517.0
66	8656.5
67	5796.0
68	4393.0
69	2990.0
70	2253.0
71	1516.0
72	1260.0
73	1004.0
74	829.5
75	472.5
76	290.0
77	176.5
78	63.0
79	48.0
80	33.0
81	23.0
82	13.0
83	7.5
84	2.0
85	2.5
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1618549.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.670114893071258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9116333103383	18.915175417486882
2	6.083297206819421	2.8798468762823033
3	2.351422838676864	1.6697534626101942
4	1.3887861970798492	1.3149091538716613
5	0.9062556349945418	1.0725587501407028
6	0.6749376233189333	0.958551065376936
7	0.5525384159360613	0.9155053451629517
8	0.43918953106610825	0.8316533328135096
9	0.3881711078328789	0.8269249248517497
>10	5.297249345684877	30.0421165656448
>50	1.6434342133051603	27.357316745863606
>100	0.3602133094418398	11.294121998144488
>500	0.0020881930946975032	0.32542008383454823
>1k	5.220482736743758E-4	0.17386218262965988
>5k	0.0	0.0
>10k+	2.610241368371879E-4	1.422284095285988
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23020	1.4222615441361366	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1733	0.10707121007766833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03422818833411902	0.0
2	0.0	0.0	0.0	0.09539408445465661	0.0
3	0.0	0.0	0.0	0.14024907494305083	0.0
4	0.0	0.0	0.0	0.18732827983582825	0.0
5	0.0	0.0	0.0	0.31676520142423864	0.0
6	0.0	0.0	0.0	0.47005064412631314	0.0
7	0.0	0.0	0.0	0.5773689891378018	0.0
8	0.0	0.0	0.0	0.7579010582935704	0.0
9	0.0	0.0	0.0	0.8383434792520955	0.0
10	0.0	0.0	0.0	0.9850798462079307	0.0
11	0.0	0.0	0.0	1.2126293365230216	0.0
12	0.0	0.0	0.0	1.3780243909822933	0.0
13	0.0	0.0	0.0	1.4501877916578367	0.0
14	0.0	0.0	0.0	1.4854663034606923	0.0
15	0.0	0.0	0.0	1.5350786414251283	0.0
16	0.0	0.0	0.0	1.6435090936388086	0.0
17	0.0	0.0	0.0	1.7681886677511771	0.0
18	0.0	0.0	0.0	1.9179524376463115	0.0
19	0.0	0.0	0.0	2.012049063698411	0.0
20	6.178373345508848E-5	0.0	0.0	2.1111501721603734	0.0
21	6.178373345508848E-5	0.0	0.0	2.2355826113389217	0.0
22	6.178373345508848E-5	0.0	0.0	2.3744106604125053	0.0
23	6.178373345508848E-5	0.0	0.0	2.5307852897873344	0.0
24	6.178373345508848E-5	0.0	0.0	2.65033681402293	0.0
25	6.178373345508848E-5	0.0	0.0	2.758952617436976	0.0
26	6.178373345508848E-5	0.0	0.0	2.8628110733749796	0.0
27	6.178373345508848E-5	0.0	0.0	2.9751956845297856	0.0
28	6.178373345508848E-5	0.0	0.0	3.081710841006358	0.0
29	6.178373345508848E-5	0.0	0.0	3.214916570335529	0.0
30	6.178373345508848E-5	0.0	0.0	3.370117308774711	0.0
31	6.178373345508848E-5	0.0	0.0	3.510860653585403	0.0
32	6.178373345508848E-5	0.0	0.0	3.633316013293388	0.0
33	6.178373345508848E-5	0.0	0.0	3.7587987759406727	0.0
34	6.178373345508848E-5	0.0	0.0	3.8921898564702087	0.0
35	6.178373345508848E-5	0.0	0.0	4.056287452526924	0.0
36	6.178373345508848E-5	0.0	0.0	4.202467765881663	0.0
37	6.178373345508848E-5	0.0	0.0	4.355444289916462	0.0
38	6.178373345508848E-5	0.0	0.0	4.520097939574273	0.0
39	6.178373345508848E-5	0.0	0.0	4.754999694170519	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGCG	30	2.1660526E-6	45.000004	1
CCGCTCG	60	0.0	45.000004	19
TCGCGAG	30	2.1660526E-6	45.000004	1
TCACCGT	25	3.891514E-5	45.0	24
ATAGCGC	25	3.891514E-5	45.0	18
CGACATA	25	3.891514E-5	45.0	17
CGTCGGA	20	7.034142E-4	45.0	17
CGCGTCA	25	3.891514E-5	45.0	39
ATACGCG	40	6.8175723E-9	45.0	1
TACCGTA	20	7.034142E-4	45.0	40
CGTTTTT	11880	0.0	43.844696	1
CGGTCTA	365	0.0	43.76712	31
CGTATGG	200	0.0	42.75	2
CGACGGT	375	0.0	42.6	28
GACCGAT	280	0.0	42.589283	9
CGTAAGG	230	0.0	42.06522	2
TTTCGCG	75	0.0	42.0	1
CACGACG	385	0.0	41.493504	26
TAGGGTA	1125	0.0	41.4	5
TCGTGCG	50	1.0822987E-9	40.5	1
>>END_MODULE