FastQCFastQC Report
Thu 26 May 2016
SRR1547714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547714_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2013212
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90670.45037482391322925No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24350.12095099770913346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG1350.045.0000041
CGAACGC207.034552E-445.028
CGACGCA253.891855E-545.031
CGTTACA406.8193913E-945.016
CAGTCGT207.034552E-445.018
CGTTTCG207.034552E-445.044
TAGTACG1000.045.01
TACCGGA253.891855E-545.014
TAATCGC207.034552E-445.024
CGTTTTT58100.042.9475061
CGACGGT4450.042.4719128
GCGTAAG800.042.18751
TACGTAG800.042.18751
TATCTCG603.6379788E-1241.2500041
CGTTAGG2300.041.0869562
CTATGCG501.0822987E-940.51
TACGGGT501.0822987E-940.54
TGTAACG501.0822987E-940.51
CGATATC501.0822987E-940.510
GGACGTA950.040.2631578