##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547714_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2013212 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58365487588987 31.0 31.0 33.0 30.0 34.0 2 31.948410301547973 31.0 31.0 34.0 30.0 34.0 3 32.01018273286668 33.0 31.0 34.0 30.0 34.0 4 35.72857950379791 37.0 35.0 37.0 33.0 37.0 5 35.65011335120196 37.0 35.0 37.0 33.0 37.0 6 35.704167767726396 37.0 35.0 37.0 33.0 37.0 7 36.06997077307308 37.0 35.0 37.0 35.0 37.0 8 35.94947526639022 37.0 35.0 37.0 35.0 37.0 9 37.713717680999316 39.0 38.0 39.0 35.0 39.0 10 37.36731253340433 39.0 37.0 39.0 34.0 39.0 11 37.03889505923867 39.0 37.0 39.0 33.0 39.0 12 36.27196639002748 38.0 35.0 39.0 33.0 39.0 13 35.99076699324264 38.0 35.0 39.0 32.0 39.0 14 36.88424020917817 39.0 35.0 41.0 32.0 41.0 15 37.13041199833897 39.0 35.0 41.0 32.0 41.0 16 37.273341804042495 39.0 35.0 41.0 33.0 41.0 17 37.18251828421448 39.0 35.0 41.0 32.0 41.0 18 37.116953405801276 38.0 35.0 41.0 32.0 41.0 19 37.08913815335891 38.0 35.0 41.0 32.0 41.0 20 36.975536108467466 38.0 35.0 41.0 32.0 41.0 21 36.81487940663974 38.0 35.0 41.0 32.0 41.0 22 36.75820032862907 38.0 35.0 41.0 32.0 41.0 23 36.69141352227187 38.0 35.0 41.0 32.0 41.0 24 36.691174103869834 38.0 35.0 41.0 32.0 41.0 25 36.609395831139494 38.0 35.0 41.0 32.0 41.0 26 36.555484469593864 38.0 35.0 40.0 32.0 41.0 27 36.47619326727637 38.0 35.0 40.0 31.0 41.0 28 36.48086043596005 38.0 35.0 40.0 31.0 41.0 29 36.4300317105203 38.0 35.0 40.0 31.0 41.0 30 36.435121586797614 38.0 35.0 40.0 31.0 41.0 31 36.27050752727482 38.0 35.0 40.0 31.0 41.0 32 36.083109975501834 38.0 35.0 40.0 30.0 41.0 33 36.0215908707081 38.0 35.0 40.0 30.0 41.0 34 35.83203209597399 38.0 35.0 40.0 30.0 41.0 35 35.77879527839095 38.0 35.0 40.0 30.0 41.0 36 35.705925655122265 38.0 35.0 40.0 29.0 41.0 37 35.428889754283205 38.0 34.0 40.0 28.0 41.0 38 35.55745296570853 38.0 35.0 40.0 29.0 41.0 39 35.48905977115177 38.0 35.0 40.0 29.0 41.0 40 35.355545267959855 37.0 34.0 40.0 28.0 41.0 41 35.32045259018921 37.0 34.0 40.0 28.0 41.0 42 35.34846007275935 37.0 34.0 40.0 28.0 41.0 43 35.249882277673684 37.0 34.0 40.0 28.0 41.0 44 35.11334275774235 37.0 34.0 40.0 27.0 41.0 45 35.104382946257026 37.0 34.0 40.0 27.0 41.0 46 34.92927024078934 37.0 34.0 40.0 27.0 41.0 47 34.81674110823897 36.0 34.0 40.0 26.0 41.0 48 34.69652525417094 36.0 34.0 40.0 26.0 41.0 49 34.7944354593555 36.0 34.0 40.0 27.0 41.0 50 34.645833126367215 36.0 34.0 40.0 26.0 41.0 51 34.03064803905401 35.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 7.0 12 5.0 13 18.0 14 14.0 15 74.0 16 134.0 17 375.0 18 807.0 19 1518.0 20 2726.0 21 4476.0 22 6625.0 23 9761.0 24 14096.0 25 20071.0 26 26949.0 27 31900.0 28 35068.0 29 39079.0 30 45939.0 31 55761.0 32 70927.0 33 94225.0 34 173707.0 35 300988.0 36 123403.0 37 161425.0 38 262334.0 39 530666.0 40 133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.99140279314846 23.385813317226404 28.689725672209388 13.93305821741575 2 31.533638782204754 24.77876150152095 30.056993500932837 13.630606215341453 3 29.652813513926997 24.666900455590373 32.02633403734927 13.653951993133361 4 26.953395866903236 26.88912047017403 30.6949292970636 15.462554365859136 5 23.12374454354534 31.22338829691061 30.367641361168126 15.28522579837593 6 22.606412042050216 39.361279388360494 27.243231214596374 10.789077354992918 7 86.3281661345154 4.2251884053939675 7.385809343476992 2.0608361166136504 8 86.27556362668214 3.9318760269658637 7.038205613715793 2.7543547326362052 9 82.22676002328618 6.076756943630378 8.38446224242653 3.31202079065692 10 47.94726039781205 30.47135622080536 12.78265776281882 8.798725618563767 11 40.55390093045342 20.007728942605148 24.304643524874677 15.133726602066746 12 38.1219166188161 19.86109758932492 26.32931852184469 15.687667270014286 13 24.430710724950973 33.655918999092 25.881029916372444 16.032340359584584 14 16.065719854640246 37.971112828653915 28.572847767646927 17.390319549058916 15 15.071289064440307 26.666342143798072 43.16659149657364 15.095777295187988 16 19.881512726925926 22.334259879237756 41.537105878566194 16.247121515270123 17 19.522484467606986 22.525744928999032 28.52128836903416 29.43048223435982 18 22.14342056375583 26.108229038968574 32.87979606718021 18.868554330095392 19 26.650248458681947 26.455336050053347 26.727239853527596 20.16717563773711 20 29.190964488588385 25.881675650651793 26.485039826903478 18.442320033856344 21 24.808067903429944 26.801598639388203 29.97727015336686 18.413063303814997 22 22.80033101332597 22.7023284184676 29.44776804429936 25.04957252390707 23 19.03247149331516 27.58586775759334 29.345891043764887 24.03576970532661 24 20.395020494612588 24.95161960091635 36.268410877741644 18.384949026729423 25 19.111996153410573 25.774831463353088 33.43771048453913 21.675461898697208 26 17.52502965410498 32.79907928226138 28.103995008970738 21.571896054662897 27 17.395584767029007 32.875971333371744 30.657526380728907 19.070917518870342 28 15.076057563733974 29.49714188073586 36.38603386031873 19.040766695211435 29 16.747962956707987 26.060742733502483 35.97236654659321 21.218927763196323 30 19.52382560803333 28.82602527702001 32.7931186581443 18.857030456802363 31 26.161377937345893 26.996908422957937 27.214520875099097 19.62719276459707 32 26.7834187358311 26.36617504763532 28.72866841644099 18.12173780009259 33 25.96110096701192 27.468095759413313 26.915843934965615 19.65495933860915 34 19.491638237801087 27.218395280775198 29.430829937433316 23.8591365439904 35 18.879879515917846 27.456075167443867 30.820549450331114 22.843495866307176 36 26.856585396868287 24.5903561075535 29.514924409351824 19.03813408622639 37 20.020395268853953 30.3245758519222 30.05600006357999 19.599028815643855 38 19.772532649318602 30.65052264739133 26.595311373069503 22.981633330220564 39 19.877638321249826 30.021726474906767 28.733486587602297 21.36714861624111 40 22.932060806313494 26.239660800750244 27.576032727800154 23.252245665136112 41 17.8228125006209 24.809160684518073 29.21465796945379 28.15336884540724 42 21.250866774090362 25.481171381851492 27.119150889225775 26.14881095483238 43 21.775352024525983 25.18497803510013 27.52109564218771 25.51857429818618 44 19.45771235220136 29.01934818588405 28.68068539229848 22.842254069616118 45 18.736824537107864 34.06233422014174 25.274139037518157 21.926702205232235 46 21.85467799715082 30.84513702481408 27.140112417370847 20.16007256066425 47 20.732242804036535 28.266471688028883 27.655408372292634 23.34587713564195 48 21.220368247357953 26.179806200241206 31.462061620932126 21.13776393146872 49 21.58903284899951 24.23058277021993 31.425602470082637 22.75478191069793 50 19.23518238516361 29.67894091630688 29.572295416478738 21.513581282050772 51 18.42851125465177 31.01883954595939 27.50683981617435 23.045809383214486 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1757.0 1 2741.0 2 3725.0 3 10115.0 4 16505.0 5 11855.0 6 7205.0 7 7485.5 8 7766.0 9 8155.5 10 8545.0 11 8697.0 12 8849.0 13 8615.5 14 8382.0 15 7781.5 16 7181.0 17 6862.5 18 6544.0 19 7959.5 20 9375.0 21 8726.5 22 8078.0 23 8360.0 24 8642.0 25 10972.0 26 15343.0 27 17384.0 28 21377.5 29 25371.0 30 30438.0 31 35505.0 32 40560.5 33 45616.0 34 53254.0 35 60892.0 36 61943.0 37 62994.0 38 74882.0 39 86770.0 40 109923.0 41 133076.0 42 159975.0 43 186874.0 44 194833.5 45 202793.0 46 193662.5 47 184532.0 48 180728.5 49 176925.0 50 163693.0 51 150461.0 52 137594.0 53 124727.0 54 112610.0 55 100493.0 56 93918.0 57 87343.0 58 79232.5 59 71122.0 60 60486.5 61 49851.0 62 43344.5 63 36838.0 64 30529.0 65 24220.0 66 19294.0 67 14368.0 68 11350.0 69 8332.0 70 6652.5 71 4973.0 72 3857.5 73 2742.0 74 2128.0 75 1092.5 76 671.0 77 579.0 78 487.0 79 367.5 80 248.0 81 182.0 82 116.0 83 89.0 84 62.0 85 45.5 86 29.0 87 19.5 88 10.0 89 7.0 90 4.0 91 5.0 92 6.0 93 5.0 94 4.0 95 3.0 96 2.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2013212.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.26077798362171 #Duplication Level Percentage of deduplicated Percentage of total 1 81.90116756205244 15.77480205012092 2 7.523730499163664 2.8982580550598938 3 2.4204403369157292 1.3985869185580917 4 1.1005389268544246 0.8478894372990545 5 0.6330653480151739 0.6096665558622238 6 0.42967553466478314 0.49655310469034053 7 0.3263349969200081 0.43998261467735056 8 0.240966544764377 0.37129624961496865 9 0.1931370466713569 0.334797279871445 >10 2.378827691039342 11.42067752534461 >50 1.2903256755813655 18.24825607958589 >100 1.5535085987836972 45.33322273016067 >500 0.0059521402247550625 0.7886431281711767 >1k 0.002070309643393065 0.585426212891573 >5k 2.5878870542413315E-4 0.45194205809177457 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9067 0.45037482391322925 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 2435 0.12095099770913346 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.96718676423546E-5 0.0 0.0 0.08116383172760742 0.0 2 4.96718676423546E-5 0.0 0.0 0.277765083856047 0.0 3 4.96718676423546E-5 0.0 0.0 0.3688632891121253 0.0 4 4.96718676423546E-5 0.0 0.0 0.5137064551572313 0.0 5 4.96718676423546E-5 0.0 0.0 0.8641911532416854 0.0 6 4.96718676423546E-5 0.0 0.0 1.1327669415838968 0.0 7 4.96718676423546E-5 0.0 0.0 1.3310570372121764 0.0 8 4.96718676423546E-5 0.0 0.0 1.7364291490414323 0.0 9 4.96718676423546E-5 0.0 0.0 1.8505751008835631 0.0 10 4.96718676423546E-5 0.0 0.0 2.0848276286849075 0.0 11 4.96718676423546E-5 0.0 0.0 2.4250799220350365 0.0 12 4.96718676423546E-5 0.0 0.0 2.6939040697154595 0.0 13 4.96718676423546E-5 0.0 0.0 2.8032815222639247 0.0 14 4.96718676423546E-5 0.0 0.0 2.8451549066864295 0.0 15 4.96718676423546E-5 0.0 0.0 2.9233384263554956 0.0 16 4.96718676423546E-5 0.0 0.0 3.0727017323560557 0.0 17 4.96718676423546E-5 0.0 0.0 3.256934689441549 0.0 18 4.96718676423546E-5 0.0 0.0 3.4837364370965402 0.0 19 4.96718676423546E-5 0.0 0.0 3.6156152456869917 0.0 20 4.96718676423546E-5 0.0 0.0 3.7634883956582814 0.0 21 4.96718676423546E-5 0.0 0.0 3.9584504761545234 0.0 22 4.96718676423546E-5 0.0 0.0 4.1571379467239415 0.0 23 4.96718676423546E-5 0.0 0.0 4.369932227703789 0.0 24 4.96718676423546E-5 0.0 0.0 4.526050907703709 0.0 25 4.96718676423546E-5 0.0 0.0 4.655495794779686 0.0 26 4.96718676423546E-5 0.0 0.0 4.789361478075831 0.0 27 4.96718676423546E-5 0.0 0.0 4.929386472959629 0.0 28 4.96718676423546E-5 0.0 0.0 5.073236201651888 0.0 29 4.96718676423546E-5 0.0 0.0 5.2356135369747445 0.0 30 4.96718676423546E-5 0.0 0.0 5.439466881778968 0.0 31 9.93437352847092E-5 0.0 0.0 5.612324981174362 0.0 32 9.93437352847092E-5 0.0 0.0 5.768393989306641 0.0 33 9.93437352847092E-5 0.0 0.0 5.923618575688998 0.0 34 9.93437352847092E-5 0.0 0.0 6.095979956407969 0.0 35 9.93437352847092E-5 0.0 0.0 6.304552128638216 0.0 36 9.93437352847092E-5 0.0 0.0 6.47298943181344 0.0 37 9.93437352847092E-5 0.0 0.0 6.647039656032251 0.0 38 9.93437352847092E-5 0.0 0.0 6.836537831087834 0.0 39 9.93437352847092E-5 0.0 0.0 7.1082926189591555 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATGCG 135 0.0 45.000004 1 CGAACGC 20 7.034552E-4 45.0 28 CGACGCA 25 3.891855E-5 45.0 31 CGTTACA 40 6.8193913E-9 45.0 16 CAGTCGT 20 7.034552E-4 45.0 18 CGTTTCG 20 7.034552E-4 45.0 44 TAGTACG 100 0.0 45.0 1 TACCGGA 25 3.891855E-5 45.0 14 TAATCGC 20 7.034552E-4 45.0 24 CGTTTTT 5810 0.0 42.947506 1 CGACGGT 445 0.0 42.47191 28 GCGTAAG 80 0.0 42.1875 1 TACGTAG 80 0.0 42.1875 1 TATCTCG 60 3.6379788E-12 41.250004 1 CGTTAGG 230 0.0 41.086956 2 CTATGCG 50 1.0822987E-9 40.5 1 TACGGGT 50 1.0822987E-9 40.5 4 TGTAACG 50 1.0822987E-9 40.5 1 CGATATC 50 1.0822987E-9 40.5 10 GGACGTA 95 0.0 40.263157 8 >>END_MODULE