FastQCFastQC Report
Thu 26 May 2016
SRR1547713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547713_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences872755
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289743.319832026170002No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10860.12443354664252854No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9420.1079340708446242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC351.2114833E-745.0000046
TCTTGCG253.8900307E-545.0000041
CGAGTAG253.8900307E-545.0000041
CGTAGCT253.8900307E-545.00000424
GCGATAA502.1827873E-1145.0000049
CGCCGGT253.8900307E-545.00000428
TAGGTCG207.032353E-445.01
GTTACCG207.032353E-445.01
GCGATAC453.8380676E-1045.09
ATGCGCG207.032353E-445.01
CGGTAAT207.032353E-445.032
TTACCGG406.8121153E-945.02
ATCGACG207.032353E-445.01
CGTTTTT111450.044.2934041
CGACGGT2450.044.08163528
ACGTAGG800.042.18752
CGGTCTA2600.041.5384631
ACGGTCT2600.041.5384630
GTCGCGT603.6379788E-1241.25000429
CCGCTCG603.6379788E-1241.25000419