##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547713_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	872755
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7239087716484	31.0	31.0	33.0	30.0	34.0
2	32.10917038573254	33.0	31.0	34.0	30.0	34.0
3	32.01117266586843	33.0	31.0	34.0	30.0	34.0
4	35.79764767890187	37.0	35.0	37.0	35.0	37.0
5	35.78527307205344	37.0	35.0	37.0	35.0	37.0
6	35.856776529495676	37.0	35.0	37.0	35.0	37.0
7	36.19834776082635	37.0	35.0	37.0	35.0	37.0
8	36.11065648435128	37.0	35.0	37.0	35.0	37.0
9	37.93773968639538	39.0	39.0	39.0	35.0	39.0
10	37.574168008203905	39.0	37.0	39.0	35.0	39.0
11	37.28376004720683	39.0	37.0	39.0	34.0	39.0
12	36.786904686882345	39.0	35.0	39.0	33.0	39.0
13	36.613270620048006	39.0	35.0	39.0	33.0	39.0
14	37.64909281528035	40.0	35.0	41.0	33.0	41.0
15	37.85543823868096	40.0	35.0	41.0	33.0	41.0
16	37.948066754129165	40.0	35.0	41.0	33.0	41.0
17	37.86069286340382	40.0	35.0	41.0	33.0	41.0
18	37.628021609730105	39.0	36.0	41.0	33.0	41.0
19	37.42778328396858	38.0	36.0	41.0	33.0	41.0
20	37.16143935010398	38.0	35.0	41.0	33.0	41.0
21	37.047764836638	38.0	35.0	41.0	33.0	41.0
22	37.02012821467651	38.0	35.0	41.0	33.0	41.0
23	36.93425760952386	38.0	35.0	41.0	33.0	41.0
24	36.86308757898838	38.0	35.0	41.0	33.0	41.0
25	36.79405331393117	38.0	35.0	41.0	33.0	41.0
26	36.76403458015136	38.0	35.0	41.0	33.0	41.0
27	36.678409175541816	38.0	35.0	41.0	32.0	41.0
28	36.644165888479584	38.0	35.0	40.0	32.0	41.0
29	36.54454572016201	38.0	35.0	40.0	32.0	41.0
30	36.4186169085253	38.0	35.0	40.0	32.0	41.0
31	36.156319929418906	38.0	35.0	41.0	31.0	41.0
32	35.7465119076946	38.0	35.0	41.0	30.0	41.0
33	35.42385320049728	38.0	35.0	41.0	27.0	41.0
34	35.02783713642431	38.0	35.0	40.0	24.0	41.0
35	34.75343939593586	38.0	34.0	40.0	23.0	41.0
36	34.589333776374815	38.0	34.0	40.0	22.0	41.0
37	34.31920527524907	37.0	34.0	40.0	21.0	41.0
38	34.38961907981049	37.0	34.0	40.0	21.0	41.0
39	34.29980464162337	37.0	34.0	40.0	21.0	41.0
40	34.19004646206553	37.0	34.0	40.0	20.0	41.0
41	34.100534514268034	37.0	34.0	40.0	18.0	41.0
42	34.1036041042446	37.0	34.0	40.0	18.0	41.0
43	34.009199603554265	37.0	34.0	40.0	18.0	41.0
44	33.845385016413545	36.0	33.0	40.0	18.0	41.0
45	33.83607427055703	36.0	34.0	40.0	18.0	41.0
46	33.660036321762696	36.0	33.0	40.0	18.0	41.0
47	33.55709448814387	36.0	33.0	40.0	18.0	41.0
48	33.415180663531004	36.0	33.0	40.0	18.0	41.0
49	33.45907270654422	36.0	33.0	40.0	18.0	41.0
50	33.32733928765805	35.0	33.0	40.0	18.0	41.0
51	32.80944136670658	35.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	1.0
12	1.0
13	1.0
14	5.0
15	27.0
16	52.0
17	143.0
18	405.0
19	736.0
20	1334.0
21	2180.0
22	3353.0
23	5448.0
24	8715.0
25	14231.0
26	20455.0
27	21912.0
28	20521.0
29	18881.0
30	19474.0
31	21791.0
32	26711.0
33	35686.0
34	62547.0
35	112308.0
36	60629.0
37	71375.0
38	111235.0
39	232539.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.573998430258207	22.244730766366278	27.408722952031212	21.772547851344306
2	37.64871011910559	23.080016728635183	27.71762980446975	11.553643347789471
3	25.383526877531494	22.44845346059318	40.01661405549095	12.15140560638438
4	22.97460341103746	25.087682110099628	39.007969017651	12.929745461211908
5	20.976906462867586	29.52352034648899	37.52061002228575	11.978963168357671
6	19.8834724521773	37.02952145791201	33.83950822395747	9.247497865953216
7	80.56854443686944	2.9908737274492845	15.1876528922779	1.2529289434033606
8	81.84599343458359	2.4254229422919376	14.720511483749736	1.0080721393747387
9	78.33939650875675	4.3527679589346375	15.663960676249348	1.6438748560592606
10	39.20332739428591	29.613007086754013	23.314905099369238	7.868760419590835
11	31.71474239620512	21.106037776924797	32.80680145057892	14.37241837629117
12	31.241872003024906	20.766538146444304	33.59098486975153	14.400604980779255
13	22.325853189039307	29.457522443297375	33.583880928782996	14.632743438880327
14	14.744802378674427	33.925557573431256	35.67759565972123	15.652044388173083
15	14.23274573047419	26.924852908319057	45.51197071343046	13.330430647776295
16	17.862974145092263	24.024153399293045	44.30911309588602	13.803759359728677
17	18.34019856660804	23.438078269388317	35.126352756500964	23.095370407502678
18	19.628074316388908	25.957456559973874	37.8745466940894	16.53992242954781
19	22.208179844286196	27.49179322948594	33.808571706836396	16.49145521939147
20	24.07296434852851	26.825283155066426	33.954889974849756	15.146862521555304
21	20.80114121374269	26.19131371347056	37.04476055708647	15.962784515700282
22	20.252075324690207	22.448911779365343	36.78970616037719	20.509306735567257
23	16.67249113439625	27.723244209428767	36.003116567650714	19.60114808852427
24	16.678678437820466	27.659079581325802	40.05179002125453	15.6104519595992
25	17.036396239494476	28.307944383017	37.34816758425904	17.307491793229485
26	16.05525032798437	32.885632279391125	32.87944497596691	18.179672416657596
27	15.584556948971933	32.70115897359511	36.62241980853733	15.091864268895625
28	13.80570721451037	29.637870880143915	40.80457860453392	15.751843300811796
29	14.987711327921351	27.25197793195112	40.549065889052486	17.211244851075044
30	16.659429049389576	29.287600758517566	38.60739841077966	15.445571781313197
31	22.665123660133716	28.189354400719562	33.161654759926904	15.983867179219827
32	22.609094190236664	29.081357311043764	33.21172608578581	15.097822412933755
33	21.517493454635034	29.86061380341562	32.352836706750466	16.26905603519888
34	17.34220944022091	30.441876586212622	33.45818700551702	18.75772696804945
35	17.467445044714726	30.120595126925654	33.315420707987926	19.0965391203717
36	23.358101643645696	28.368442460942646	32.26793315420708	16.005522741204576
37	18.251743043580387	32.106834105791435	33.162685977164266	16.478736873463916
38	18.32209497510756	32.5496846193949	29.989286798700665	19.13893360679687
39	17.57618117341064	32.05149211405263	31.516404947551145	18.85592176498559
40	19.855056688303133	30.045430848290756	30.84027017891619	19.25924228448992
41	17.165642133244724	28.723983248448874	31.945047579217533	22.16532703908886
42	19.38470704836982	29.58745581520587	30.087939914408967	20.93989722201534
43	20.331822791046744	29.952735876620583	29.651735023001873	20.063706309330797
44	18.775028501698642	32.49124897594399	30.3129744315415	18.420748090815863
45	17.69179208368901	35.68126220989854	27.941633104364914	18.68531260204754
46	20.480088913841797	32.91038149308798	29.464855543651996	17.14467404941822
47	19.507880218388895	31.875612285234688	30.072700815234516	18.5438066811419
48	20.355999106278393	30.252132614536727	31.710273788176522	17.681594491008358
49	19.79879805902	28.442002623874966	31.407095920390027	20.352103396715
50	18.039083133296284	32.30528613413844	31.316692542580675	18.33893818998459
51	17.48635069406649	33.540798964199574	29.541051039524262	19.43179930220967
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1167.0
1	1352.0
2	1537.0
3	19772.5
4	38008.0
5	23998.0
6	9988.0
7	10186.0
8	10384.0
9	11317.5
10	12251.0
11	12847.5
12	13444.0
13	13303.5
14	13163.0
15	12695.0
16	12227.0
17	11457.0
18	10687.0
19	10056.0
20	9425.0
21	8737.0
22	8049.0
23	8296.0
24	8543.0
25	8722.0
26	9849.0
27	10797.0
28	12416.5
29	14036.0
30	16078.0
31	18120.0
32	20333.0
33	22546.0
34	25983.0
35	29420.0
36	29847.5
37	30275.0
38	35212.0
39	40149.0
40	47726.0
41	55303.0
42	62524.0
43	69745.0
44	72277.5
45	74810.0
46	71810.5
47	68811.0
48	65992.0
49	63173.0
50	57713.5
51	52254.0
52	48153.0
53	44052.0
54	39130.0
55	34208.0
56	30333.5
57	26459.0
58	23752.0
59	21045.0
60	18654.5
61	16264.0
62	13197.5
63	10131.0
64	8418.5
65	6706.0
66	4957.0
67	3208.0
68	2354.0
69	1500.0
70	1244.5
71	989.0
72	722.5
73	456.0
74	379.0
75	200.0
76	98.0
77	79.5
78	61.0
79	51.5
80	42.0
81	24.0
82	6.0
83	7.0
84	8.0
85	4.0
86	0.0
87	1.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	872755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.337096759775566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32197557500521	21.344061100063744
2	7.586821624997213	4.299769969734074
3	3.440984356524136	2.9252251997909546
4	2.041775744195431	2.314319873001148
5	1.3386643849236517	1.8966931102223479
6	0.9478874126110166	1.611622639711901
7	0.7897397370920131	1.5665251941511262
8	0.644249059205938	1.4604918342490434
9	0.5514628212059542	1.4064169791538743
>10	6.626432605919259	43.28070886160748
>50	0.6599890259104352	11.883888408030305
>100	0.047171119345768425	2.1675161466628743
>500	0.0020332379028348458	0.378883205222091
>1k	4.0664758056696917E-4	0.12514208055693152
>5k	0.0	0.0
>10k+	4.0664758056696917E-4	3.3387353978421124
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28974	3.319832026170002	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1086	0.12443354664252854	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	942	0.1079340708446242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020280605668257415	0.0
2	0.0	0.0	0.0	0.06210219362822327	0.0
3	0.0	0.0	0.0	0.09659068123356497	0.0
4	0.0	0.0	0.0	0.12867299528504564	0.0
5	0.0	0.0	0.0	0.23271135656627576	0.0
6	0.0	0.0	0.0	0.4007997662574262	0.0
7	0.0	0.0	0.0	0.5311914569380869	0.0
8	0.0	0.0	0.0	0.7142898064176086	0.0
9	0.0	0.0	0.0	0.8492646848199094	0.0
10	0.0	0.0	0.0	1.0125407474033377	0.0
11	0.0	0.0	0.0	1.2295546860229962	0.0
12	0.0	0.0	0.0	1.3623525502575178	0.0
13	0.0	0.0	0.0	1.420788193708429	0.0
14	0.0	0.0	0.0	1.4509226529782127	0.0
15	0.0	0.0	0.0	1.4872444156722104	0.0
16	0.0	0.0	0.0	1.5451071606579165	0.0
17	0.0	0.0	0.0	1.6175215266598302	0.0
18	0.0	0.0	0.0	1.7086123826274269	0.0
19	0.0	0.0	0.0	1.7657876494548872	0.0
20	0.0	0.0	0.0	1.8235358147475522	0.0
21	0.0	0.0	0.0	1.8887316600878827	0.0
22	0.0	0.0	0.0	1.9737497923243064	0.0
23	0.0	0.0	0.0	2.055215954076459	0.0
24	0.0	0.0	0.0	2.1223596541984864	0.0
25	0.0	0.0	0.0	2.1747225739182245	0.0
26	0.0	0.0	0.0	2.234991492457792	0.0
27	0.0	0.0	0.0	2.307176699073623	0.0
28	0.0	0.0	0.0	2.365039444059329	0.0
29	0.0	0.0	0.0	2.438599606991653	0.0
30	0.0	0.0	0.0	2.5235031595350357	0.0
31	0.0	0.0	0.0	2.603021466505491	0.0
32	0.0	0.0	0.0	2.676581629437815	0.0
33	0.0	0.0	0.0	2.748537676667564	0.0
34	0.0	0.0	0.0	2.8256498100841587	0.0
35	0.0	0.0	0.0	2.922469650703806	0.0
36	0.0	0.0	0.0	3.0032483342977123	0.0
37	0.0	0.0	0.0	3.0950266684235554	0.0
38	0.0	0.0	0.0	3.193106885666653	0.0
39	0.0	0.0	0.0	3.3578724842596146	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	35	1.2114833E-7	45.000004	6
TCTTGCG	25	3.8900307E-5	45.000004	1
CGAGTAG	25	3.8900307E-5	45.000004	1
CGTAGCT	25	3.8900307E-5	45.000004	24
GCGATAA	50	2.1827873E-11	45.000004	9
CGCCGGT	25	3.8900307E-5	45.000004	28
TAGGTCG	20	7.032353E-4	45.0	1
GTTACCG	20	7.032353E-4	45.0	1
GCGATAC	45	3.8380676E-10	45.0	9
ATGCGCG	20	7.032353E-4	45.0	1
CGGTAAT	20	7.032353E-4	45.0	32
TTACCGG	40	6.8121153E-9	45.0	2
ATCGACG	20	7.032353E-4	45.0	1
CGTTTTT	11145	0.0	44.293404	1
CGACGGT	245	0.0	44.081635	28
ACGTAGG	80	0.0	42.1875	2
CGGTCTA	260	0.0	41.53846	31
ACGGTCT	260	0.0	41.53846	30
GTCGCGT	60	3.6379788E-12	41.250004	29
CCGCTCG	60	3.6379788E-12	41.250004	19
>>END_MODULE