FastQCFastQC Report
Thu 26 May 2016
SRR1547712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547712_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1348781
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58600.43446638112488234No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14270.10579923649576914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT253.891166E-545.00000427
CTCGGTA302.1657834E-645.00000428
ATATACG351.2121382E-745.0000041
CGACTTA302.1657834E-645.00000419
CGCGGAT207.0337235E-445.045
CTATCGC207.0337235E-445.044
CCGGCTA207.0337235E-445.033
TTCGTAG453.8562575E-1045.01
TGTCGGT207.0337235E-445.036
GCGTAAA207.0337235E-445.021
CACGACG3750.044.99999626
CGACGGT3800.044.40789428
ACGACGG3950.042.7215227
CGTAAGG1900.042.631582
CGGTCTA4050.042.2222231
TTAAGCG750.042.01
CGTTTTT40450.041.829421
ACGGTCT4050.041.66666430
TACGGGT603.6379788E-1241.2500044
GCGCGAC950.040.2631579