##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547712_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1348781
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.633754479044413	31.0	31.0	33.0	30.0	34.0
2	32.009280972967446	33.0	31.0	34.0	30.0	34.0
3	32.07579065837968	33.0	31.0	34.0	30.0	34.0
4	35.782151438966	37.0	35.0	37.0	35.0	37.0
5	35.71466828195237	37.0	35.0	37.0	33.0	37.0
6	35.763118697549864	37.0	35.0	37.0	33.0	37.0
7	36.08642173933352	37.0	35.0	37.0	35.0	37.0
8	35.9684181494253	37.0	35.0	37.0	35.0	37.0
9	37.721409924961876	39.0	38.0	39.0	35.0	39.0
10	37.41600674979852	39.0	37.0	39.0	35.0	39.0
11	37.11175498468617	39.0	37.0	39.0	33.0	39.0
12	36.41942020239016	38.0	35.0	39.0	33.0	39.0
13	36.18499741618543	38.0	35.0	39.0	32.0	39.0
14	37.09325976566989	39.0	35.0	41.0	32.0	41.0
15	37.31897098194592	39.0	35.0	41.0	32.0	41.0
16	37.440384317394745	39.0	35.0	41.0	33.0	41.0
17	37.349157498511616	39.0	35.0	41.0	33.0	41.0
18	37.26213373409026	39.0	35.0	41.0	32.0	41.0
19	37.235372532679506	39.0	35.0	41.0	32.0	41.0
20	37.14641368761867	39.0	35.0	41.0	32.0	41.0
21	37.0085343728893	39.0	35.0	41.0	32.0	41.0
22	36.987395285075934	39.0	35.0	41.0	32.0	41.0
23	36.91920259849449	38.0	35.0	41.0	32.0	41.0
24	36.921770843450496	38.0	35.0	41.0	32.0	41.0
25	36.84342750972915	38.0	35.0	41.0	32.0	41.0
26	36.797296225258215	38.0	35.0	41.0	32.0	41.0
27	36.725432816743414	38.0	35.0	41.0	32.0	41.0
28	36.723933685305475	38.0	35.0	41.0	32.0	41.0
29	36.64313702521017	38.0	35.0	41.0	31.0	41.0
30	36.64632286486835	38.0	35.0	41.0	31.0	41.0
31	36.49160612434487	38.0	35.0	41.0	31.0	41.0
32	36.306295091642006	38.0	35.0	41.0	31.0	41.0
33	36.23177521035661	38.0	35.0	41.0	30.0	41.0
34	36.04641302034948	38.0	35.0	41.0	30.0	41.0
35	35.93775861314772	38.0	35.0	41.0	30.0	41.0
36	35.85268549898019	38.0	35.0	41.0	30.0	41.0
37	35.626696995286856	38.0	35.0	40.0	29.0	41.0
38	35.73282319368378	38.0	35.0	40.0	29.0	41.0
39	35.67055066760282	38.0	35.0	40.0	29.0	41.0
40	35.54813420414433	38.0	35.0	40.0	28.0	41.0
41	35.50123407728905	38.0	35.0	40.0	28.0	41.0
42	35.53655041107489	38.0	35.0	40.0	29.0	41.0
43	35.457919410193355	38.0	35.0	40.0	28.0	41.0
44	35.3165562089027	38.0	34.0	40.0	27.0	41.0
45	35.30593254205093	37.0	34.0	40.0	28.0	41.0
46	35.155453702268936	37.0	34.0	40.0	27.0	41.0
47	35.043532641696466	37.0	34.0	40.0	27.0	41.0
48	34.921588456539645	37.0	34.0	40.0	26.0	41.0
49	34.9836726644281	37.0	34.0	40.0	27.0	41.0
50	34.86243726742889	37.0	34.0	40.0	27.0	41.0
51	34.257124025323606	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	10.0
12	7.0
13	10.0
14	17.0
15	57.0
16	96.0
17	264.0
18	481.0
19	1067.0
20	1705.0
21	2870.0
22	4474.0
23	6285.0
24	9285.0
25	13060.0
26	17439.0
27	20679.0
28	22306.0
29	24725.0
30	29075.0
31	35698.0
32	44527.0
33	59698.0
34	111420.0
35	181229.0
36	82247.0
37	111559.0
38	183922.0
39	384465.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.453406446265184	23.657287580415204	28.45903078409319	13.430275189226421
2	32.09957732204116	24.699117202866887	29.483437266687474	13.717868208404477
3	30.420209062850084	24.382905749710293	31.277649966896032	13.919235220543587
4	27.210495996014178	26.677718621481173	30.046464177653746	16.0653212048509
5	24.418790003714467	31.14308401438039	29.159959993505247	15.278165988399897
6	23.23987363404437	39.79563769062583	26.314724184281957	10.649764491047844
7	85.69841953586239	4.865875186557343	7.181373403095091	2.2543318744851835
8	86.29392021388202	4.149376362804636	7.236905027576753	2.319798395736595
9	81.55571586491803	6.476514719587538	8.481584482580939	3.4861849329134977
10	46.32449597080623	30.320415249028564	13.945555282881358	9.409533497283844
11	38.98979893696605	22.232890291307484	23.98543573789963	14.791875033826841
12	36.14767705061088	21.52217446716702	26.99296624136906	15.337182240853037
13	25.355487658856408	31.619736636266378	26.18060307789033	16.844172626986886
14	17.39696807710073	35.398185472660124	30.040903601103512	17.163942849135626
15	16.693295649923893	27.02788666210452	40.955722241045805	15.32309544692578
16	22.022700497708673	23.55400913862221	38.67536686830553	15.747923495363592
17	21.85862641896646	23.479126707745735	28.20443051911318	26.457816354174625
18	23.42411407040876	25.707583366017168	32.039745518360654	18.82855704521342
19	27.369676767392185	26.37930101328533	27.70635114225363	18.54467107706885
20	28.837149989509047	26.81369325338954	27.10832966953123	17.240827087570185
21	25.125872917842113	26.391015294551156	30.186516565698952	18.296595221907783
22	23.09470551557295	23.35783199792998	30.182512950582783	23.36494953591428
23	19.601847890799174	27.478960631859433	30.71358508164038	22.205606395701007
24	21.075845522734973	25.384995785082975	35.10888721000667	18.430271482175385
25	20.541288763705897	26.44550894474344	32.464870130881145	20.54833216066952
26	18.58062947209369	32.25823910627448	27.630134173005104	21.530997248626722
27	18.36324799948991	31.85987940221578	31.447581186271158	18.329291412023153
28	16.712646456318705	29.241959962366014	35.25894863584229	18.786444945472986
29	17.750917309778234	27.253720211064657	34.97387641136701	20.0214860677901
30	20.507999445425167	28.468594975759594	33.03709052841047	17.986315050404773
31	27.10432605441506	26.56724850068321	27.833502992702297	18.49492245219943
32	27.412159572235968	27.823049108787863	27.581720086507744	17.18307123246843
33	26.431496291836854	27.782716393543506	27.175946280382064	18.609841034237583
34	20.50659076603244	28.588703429244628	29.715498661383872	21.189207143339058
35	20.096071934583897	28.362425034160477	30.244198279780036	21.29730475147559
36	26.25363198325006	26.975691383553002	28.895350690734816	17.87532594246212
37	20.735834801943383	31.05789598163082	29.150395801838847	19.055873414586948
38	20.40346060628078	31.445060391568386	26.596089357723752	21.555389644427077
39	20.22003572114376	29.76673010666669	28.853683437118406	21.15955073507115
40	23.097226310275722	27.296425438970452	27.428248173721308	22.17810007703252
41	18.93131649986173	25.344663069838617	29.595686771981516	26.12833365831814
42	21.14931927421872	25.814049871698963	28.337217087132753	24.69941376694956
43	22.021884946481304	26.729839759012027	27.63613959567936	23.61213569882731
44	19.864826091114864	29.41863801462209	29.125039572769783	21.59149632149326
45	19.44207399125581	32.84899475897125	26.089928609611196	21.619002640161746
46	21.946038682336123	30.246125946317452	27.502611617453095	20.30522375389333
47	20.926599648126714	29.273247473088666	27.493418130890042	22.30673474789458
48	21.910228569352622	27.458201146071897	30.302843827129834	20.328726457445647
49	21.57132996387108	25.251022960732687	31.1266247077917	22.051022367604524
50	20.02422928555488	29.737073698398774	29.048970885562593	21.18972613048375
51	18.859696273894723	31.68490659343511	27.64948497939992	21.80591215327025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	807.0
1	1720.0
2	2633.0
3	6954.5
4	11276.0
5	8219.5
6	5163.0
7	5245.5
8	5328.0
9	5562.5
10	5797.0
11	5753.5
12	5710.0
13	5394.0
14	5078.0
15	4969.5
16	4861.0
17	4902.5
18	4944.0
19	5021.5
20	5099.0
21	5532.0
22	5965.0
23	7303.5
24	8642.0
25	10190.0
26	13839.5
27	15941.0
28	16964.0
29	17987.0
30	22121.0
31	26255.0
32	29786.0
33	33317.0
34	37647.5
35	41978.0
36	46077.0
37	50176.0
38	55911.0
39	61646.0
40	77251.0
41	92856.0
42	105942.0
43	119028.0
44	124830.5
45	130633.0
46	121078.0
47	111523.0
48	107937.0
49	104351.0
50	99087.0
51	93823.0
52	87050.0
53	80277.0
54	73928.0
55	67579.0
56	63726.0
57	59873.0
58	54588.5
59	49304.0
60	43268.5
61	37233.0
62	32752.0
63	28271.0
64	23719.5
65	19168.0
66	15144.0
67	11120.0
68	8625.0
69	6130.0
70	4645.5
71	3161.0
72	2612.0
73	2063.0
74	1570.5
75	796.0
76	514.0
77	386.0
78	258.0
79	176.5
80	95.0
81	71.5
82	48.0
83	37.0
84	26.0
85	17.5
86	9.0
87	8.5
88	8.0
89	5.5
90	3.0
91	3.0
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1348781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.82928053994325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07990639953717	16.077562101487626
2	5.632429795522527	2.233740610739028
3	1.7749522727999119	1.0558807968707804
4	1.0157052351170661	0.8056281621210131
5	0.6293425137710055	0.6239704630639183
6	0.5014823125075618	0.5966420076319162
7	0.3977143275061134	0.5520472282411515
8	0.34734136123733106	0.5510023436080647
9	0.31109284421635547	0.5551872553741477
>10	5.215731083758248	26.672916988416002
>50	2.542607541709696	35.97908929572988
>100	0.5494378938617189	13.576042530138862
>500	0.0015042789701895105	0.17688460438163267
>1k	3.760697425473776E-4	0.10641413594122676
>5k	3.760697425473776E-4	0.4369914762547924
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5860	0.43446638112488234	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1427	0.10579923649576914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4828204133955031E-4	0.0	0.0	0.02891499806121231	0.0
2	1.4828204133955031E-4	0.0	0.0	0.08140684069541312	0.0
3	1.4828204133955031E-4	0.0	0.0	0.1115822361080116	0.0
4	1.4828204133955031E-4	0.0	0.0	0.15161838726969018	0.0
5	1.4828204133955031E-4	0.0	0.0	0.25793661091014775	0.0
6	1.4828204133955031E-4	0.0	0.0	0.3572114375869767	0.0
7	1.4828204133955031E-4	0.0	0.0	0.4232710870037463	0.0
8	1.4828204133955031E-4	0.0	0.0	0.5527213090931737	0.0
9	1.4828204133955031E-4	0.0	0.0	0.6056579978513932	0.0
10	1.4828204133955031E-4	0.0	0.0	0.70233788880478	0.0
11	1.4828204133955031E-4	0.0	0.0	0.8661154034643134	0.0
12	1.4828204133955031E-4	0.0	0.0	1.0039435608894254	0.0
13	1.4828204133955031E-4	0.0	0.0	1.062440826197878	0.0
14	1.4828204133955031E-4	0.0	0.0	1.0925420805898067	0.0
15	1.4828204133955031E-4	0.0	0.0	1.1357662956402856	0.0
16	1.4828204133955031E-4	0.0	0.0	1.2178404055217267	0.0
17	1.4828204133955031E-4	0.0	0.0	1.315039283619802	0.0
18	1.4828204133955031E-4	0.0	0.0	1.4322562372987164	0.0
19	1.4828204133955031E-4	0.0	0.0	1.5040247453070588	0.0
20	1.4828204133955031E-4	0.0	0.0	1.585728150085151	0.0
21	1.4828204133955031E-4	0.0	0.0	1.6876720535060918	0.0
22	1.4828204133955031E-4	0.0	0.0	1.7976231871593684	0.0
23	1.4828204133955031E-4	0.0	0.0	1.914840140838283	0.0
24	1.4828204133955031E-4	0.0	0.0	2.0009178658358917	0.0
25	1.4828204133955031E-4	0.0	0.0	2.0795073477458534	0.0
26	1.4828204133955031E-4	0.0	0.0	2.161655598647964	0.0
27	1.4828204133955031E-4	0.0	0.0	2.2663427198336867	0.0
28	1.4828204133955031E-4	0.0	0.0	2.3585741495468873	0.0
29	1.4828204133955031E-4	0.0	0.0	2.4666717576834194	0.0
30	1.4828204133955031E-4	0.0	0.0	2.5950098644628	0.0
31	1.4828204133955031E-4	0.0	0.0	2.701476370144597	0.0
32	2.2242306200932546E-4	0.0	0.0	2.7999356455940587	0.0
33	2.2242306200932546E-4	0.0	0.0	2.9021019720770087	0.0
34	2.2242306200932546E-4	0.0	0.0	3.022729412706733	0.0
35	2.2242306200932546E-4	0.0	0.0	3.173087402625037	0.0
36	2.2242306200932546E-4	0.0	0.0	3.29082334344864	0.0
37	2.2242306200932546E-4	0.0	0.0	3.4231650653441887	0.0
38	2.2242306200932546E-4	0.0	0.0	3.5823458367221956	0.0
39	2.2242306200932546E-4	0.0	0.0	3.895220943948647	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCATT	25	3.891166E-5	45.000004	27
CTCGGTA	30	2.1657834E-6	45.000004	28
ATATACG	35	1.2121382E-7	45.000004	1
CGACTTA	30	2.1657834E-6	45.000004	19
CGCGGAT	20	7.0337235E-4	45.0	45
CTATCGC	20	7.0337235E-4	45.0	44
CCGGCTA	20	7.0337235E-4	45.0	33
TTCGTAG	45	3.8562575E-10	45.0	1
TGTCGGT	20	7.0337235E-4	45.0	36
GCGTAAA	20	7.0337235E-4	45.0	21
CACGACG	375	0.0	44.999996	26
CGACGGT	380	0.0	44.407894	28
ACGACGG	395	0.0	42.72152	27
CGTAAGG	190	0.0	42.63158	2
CGGTCTA	405	0.0	42.22222	31
TTAAGCG	75	0.0	42.0	1
CGTTTTT	4045	0.0	41.82942	1
ACGGTCT	405	0.0	41.666664	30
TACGGGT	60	3.6379788E-12	41.250004	4
GCGCGAC	95	0.0	40.263157	9
>>END_MODULE