FastQCFastQC Report
Thu 26 May 2016
SRR1547711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547711_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2499503
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371691.4870556266585797No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA126050.5043002548906722No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT28060.11226231774876846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG850.045.01
CGACGGT3950.043.8607628
CGTTTTT202800.043.7684941
CGGTCTA4100.042.80487431
CACGACG4200.040.71428726
GCGCGAC900.040.09
TCACGAC4250.039.7058825
CTCAACT25500.039.6176452
ACGGTCT4500.039.530
ACGGGAC8850.039.1525425
GCATTAG25050.039.07185716
GACGGTC4550.039.06593329
ACTTGAC25650.039.0350886
CGCATCG750.039.021
ACGGGTA1100.038.8636365
TTGACCC25650.038.5964938
CTATGCG1050.038.571431
CCGCTCG1050.038.5714319
AGGGATT55100.038.3847586
GCTCAAC26650.038.245781
Produced by FastQC (version 0.11.5)