##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547711_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2499503
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68174513093203	31.0	31.0	33.0	30.0	34.0
2	32.074636837803354	33.0	31.0	34.0	30.0	34.0
3	32.058221974528536	33.0	31.0	34.0	30.0	34.0
4	35.80275518773132	37.0	35.0	37.0	35.0	37.0
5	35.76586785452948	37.0	35.0	37.0	35.0	37.0
6	35.828781561774484	37.0	35.0	37.0	35.0	37.0
7	36.15726406409594	37.0	35.0	37.0	35.0	37.0
8	36.05211836113019	37.0	35.0	37.0	35.0	37.0
9	37.845620109277725	39.0	38.0	39.0	35.0	39.0
10	37.5091804250685	39.0	37.0	39.0	35.0	39.0
11	37.228982721765085	39.0	37.0	39.0	34.0	39.0
12	36.877020751725446	39.0	35.0	39.0	33.0	39.0
13	36.77556818295477	39.0	35.0	39.0	33.0	39.0
14	37.90437618998657	40.0	36.0	41.0	33.0	41.0
15	38.05012116408742	40.0	36.0	41.0	33.0	41.0
16	38.0917558410612	40.0	36.0	41.0	33.0	41.0
17	37.98513544492645	40.0	36.0	41.0	33.0	41.0
18	37.795766998479294	39.0	36.0	41.0	33.0	41.0
19	37.70139023637899	39.0	36.0	41.0	33.0	41.0
20	37.54457546160177	39.0	35.0	41.0	33.0	41.0
21	37.430323548321404	39.0	35.0	41.0	33.0	41.0
22	37.38339501892976	39.0	35.0	41.0	33.0	41.0
23	37.27889744481203	39.0	35.0	41.0	33.0	41.0
24	37.24450460751597	39.0	35.0	41.0	33.0	41.0
25	37.16616903440404	39.0	35.0	41.0	33.0	41.0
26	37.14652872991151	39.0	35.0	41.0	33.0	41.0
27	37.05079089723037	39.0	35.0	41.0	32.0	41.0
28	37.0138395513028	39.0	35.0	41.0	32.0	41.0
29	36.93686344845355	39.0	35.0	41.0	32.0	41.0
30	36.88304875009152	39.0	35.0	41.0	32.0	41.0
31	36.71184631504743	39.0	35.0	41.0	31.0	41.0
32	36.41648799781397	39.0	35.0	41.0	30.0	41.0
33	36.21113437351345	39.0	35.0	41.0	30.0	41.0
34	35.90548841109612	39.0	35.0	41.0	29.0	41.0
35	35.69164309864801	39.0	35.0	41.0	27.0	41.0
36	35.56837779350535	39.0	35.0	41.0	26.0	41.0
37	35.335767550589054	38.0	35.0	41.0	24.0	41.0
38	35.414161135233684	39.0	35.0	41.0	25.0	41.0
39	35.3268261730432	38.0	35.0	41.0	24.0	41.0
40	35.23855102394356	38.0	35.0	41.0	24.0	41.0
41	35.157394490024615	38.0	35.0	41.0	24.0	41.0
42	35.162272259725235	38.0	35.0	41.0	24.0	41.0
43	35.065712263598	38.0	35.0	40.0	23.0	41.0
44	34.938064887299596	38.0	34.0	40.0	23.0	41.0
45	34.91778045475441	38.0	34.0	40.0	23.0	41.0
46	34.73609113491762	38.0	34.0	40.0	23.0	41.0
47	34.631793200488254	38.0	34.0	40.0	23.0	41.0
48	34.506515895359996	38.0	34.0	40.0	23.0	41.0
49	34.55414536409838	38.0	34.0	40.0	23.0	41.0
50	34.45179101605399	37.0	34.0	40.0	23.0	41.0
51	33.87668108419954	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	8.0
13	14.0
14	25.0
15	58.0
16	155.0
17	385.0
18	919.0
19	1784.0
20	3300.0
21	5483.0
22	8447.0
23	12613.0
24	19622.0
25	30847.0
26	44625.0
27	50605.0
28	49418.0
29	48310.0
30	51922.0
31	60020.0
32	72884.0
33	94782.0
34	158531.0
35	251842.0
36	166245.0
37	219725.0
38	365716.0
39	781019.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.975637956825818	22.998172036600874	28.179842152619937	17.846347853953365
2	34.877613669597515	23.508073404992913	28.235373192190604	13.378939733218964
3	27.316910601827644	23.963804004236042	36.347865955752	12.371419438184311
4	25.10671121418938	25.71203155187251	34.31586199336428	14.865395240573825
5	22.600012882561053	30.94215129967838	33.04540942739417	13.412426390366406
6	20.97693021372649	39.43668001198638	29.84477314090041	9.741616633386716
7	83.3234847087601	3.3746308766182715	10.995465898620646	2.306418516000981
8	84.01762270339344	2.8889743280964253	11.566699459852618	1.5267035086575211
9	79.92688946562576	5.520657506712334	12.239113135691374	2.3133398919705237
10	38.85224382607262	37.24332397280579	16.179336452086677	7.7250957490349075
11	28.834052209579262	23.643020232422206	31.917065112544375	15.605862445454155
12	28.089264145712168	21.9188774728416	33.1046211986943	16.88723718275193
13	23.357243419991892	26.759799848209827	32.39996111226912	17.482995619529163
14	16.72620516958771	30.963435530983556	33.68633684376454	18.624022455664186
15	16.378456037060168	27.783803420119916	40.4032721705075	15.434468372312416
16	21.916276955858823	25.781325327475102	37.55166527105588	14.750732445610188
17	20.971889211575263	24.996889381609062	32.00824323875586	22.022978168059808
18	21.04146304285292	27.11038954544163	34.915301161870985	16.93284624983447
19	23.284348928567	26.98564474617554	32.40980306885008	17.320203256407375
20	25.016173215235188	27.750396778879644	32.09638076049519	15.137049245389983
21	23.747480999222645	26.387165768554787	33.53154607135899	16.333807160863582
22	22.842821152845186	22.7129553355207	34.631844810748376	19.812378700885734
23	18.973932017685115	26.706309214271794	34.66889217576454	19.650866592278547
24	18.677633113462957	26.577883683276237	37.485012020389654	17.259471182871156
25	18.879633271094292	28.609727613849635	34.54326720151966	17.96737191353641
26	18.58801529744113	31.621166287858028	31.212405026119193	18.578413388581648
27	17.416102321141445	30.592041697889538	34.78759577403988	17.20426020692914
28	17.041067764271535	27.851056790089867	37.862287022660105	17.24558842297849
29	17.365332228046938	26.9761628611768	37.234722262785844	18.423782647990418
30	18.73892529834931	28.135953427541395	35.26917151129644	17.85594976281285
31	23.323036619679993	26.540316214863513	31.811204067368593	18.3254430980879
32	22.65718424822855	28.19728562038133	32.20156167046009	16.943968460930034
33	22.368046767697418	27.67834245447995	31.05753423780648	18.896076540016153
34	19.357928356157203	28.36747945491564	32.507222435820246	19.767369753106916
35	19.73172266646609	28.083823064025125	32.512423469785794	19.672030799722982
36	22.682389258984685	27.317230665456293	31.985398697261015	18.014981378298007
37	19.8623886428622	30.250533806120654	31.109264521786933	18.777813029230213
38	19.716439628198085	30.993281464355114	29.250134926823453	20.04014398062335
39	19.566689857943757	30.142752379172983	30.499423285349124	19.791134477534133
40	21.27326912590223	27.47542211391625	30.803723780287523	20.447584979894003
41	18.67291217494038	27.60740835278053	31.115785818220665	22.603893654058428
42	20.0549069154948	27.54951684394858	29.730310385704676	22.665265854851945
43	20.448585178733534	28.406167146028633	29.836291454741204	21.308956220496636
44	19.782332727746276	29.91578725850699	30.333830365476654	19.968049648270075
45	19.36845044794905	31.678457677386263	28.68378233592838	20.2693095387363
46	21.138922417776655	30.534230204964746	29.284701798717585	19.042145578541014
47	19.95344674521295	29.206726297187878	30.442972062846096	20.396854894753076
48	20.35128583562412	28.42040997750353	31.499062013528288	19.729242173344062
49	20.43450237907296	27.54587612017269	31.56731558233777	20.45230591841658
50	19.11920089713835	30.13014987379491	30.614686199616486	20.135963029450256
51	18.679633511142015	31.10070281972056	29.71490732357593	20.504756345561496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1944.0
1	2984.5
2	4025.0
3	31416.5
4	58808.0
5	40002.5
6	21197.0
7	21072.0
8	20947.0
9	21592.5
10	22238.0
11	22488.5
12	22739.0
13	21776.5
14	20814.0
15	19987.5
16	19161.0
17	18197.0
18	17233.0
19	16729.0
20	16225.0
21	16461.5
22	16698.0
23	18539.0
24	20380.0
25	23569.0
26	31001.0
27	35244.0
28	42391.5
29	49539.0
30	61232.5
31	72926.0
32	77649.0
33	82372.0
34	89927.5
35	97483.0
36	102382.0
37	107281.0
38	119617.0
39	131953.0
40	146050.0
41	160147.0
42	173048.5
43	185950.0
44	190110.5
45	194271.0
46	191521.0
47	188771.0
48	180842.5
49	172914.0
50	163977.0
51	155040.0
52	144882.5
53	134725.0
54	124073.5
55	113422.0
56	103031.0
57	92640.0
58	81695.5
59	70751.0
60	64198.5
61	57646.0
62	48671.5
63	39697.0
64	34323.5
65	28950.0
66	23336.0
67	17722.0
68	13706.5
69	9691.0
70	7495.0
71	5299.0
72	4098.0
73	2897.0
74	2141.5
75	1120.0
76	854.0
77	635.5
78	417.0
79	266.5
80	116.0
81	128.0
82	140.0
83	92.0
84	44.0
85	29.5
86	15.0
87	12.5
88	10.0
89	8.0
90	6.0
91	6.5
92	7.0
93	7.0
94	7.0
95	4.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2499503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.57901098379631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91906215754287	16.446552579547745
2	5.651942805714874	2.3262278615718985
3	2.0822632826288845	1.285527568931267
4	1.2485225313954618	1.0277343554841751
5	0.8644447331190352	0.8894708828870745
6	0.6818151834171657	0.8418649289076571
7	0.5522930805689494	0.7955951759912178
8	0.44424817032475816	0.7313750381315689
9	0.39412224769189874	0.7299581457789065
>10	5.588829262698609	28.579716187884824
>50	2.047643354977606	29.686935282662475
>100	0.5208834897549178	13.937586431687746
>500	0.002554305107236734	0.36884507277598183
>1k	9.824250412448978E-4	0.34001515204705446
>5k	0.0	0.0
>10k+	3.92970016497959E-4	2.012595335710559
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37169	1.4870556266585797	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	12605	0.5043002548906722	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	2806	0.11226231774876846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.001590716234387E-5	0.0	0.0	0.01200238607435158	0.0
2	8.001590716234387E-5	0.0	0.0	0.04764947271517578	0.0
3	8.001590716234387E-5	0.0	0.0	0.07577506408273965	0.0
4	8.001590716234387E-5	0.0	0.0	0.11046195983761571	0.0
5	8.001590716234387E-5	0.0	0.0	0.20676110410749657	0.0
6	8.001590716234387E-5	0.0	0.0	0.34750908480605947	0.0
7	8.001590716234387E-5	0.0	0.0	0.45016949369534665	0.0
8	8.001590716234387E-5	0.0	0.0	0.6064405603834042	0.0
9	8.001590716234387E-5	0.0	0.0	0.702499656931798	0.0
10	8.001590716234387E-5	0.0	0.0	0.812081441790628	0.0
11	8.001590716234387E-5	0.0	0.0	0.9545897724467625	0.0
12	8.001590716234387E-5	0.0	0.0	1.060370801715381	0.0
13	8.001590716234387E-5	0.0	0.0	1.1205427639014636	0.0
14	8.001590716234387E-5	0.0	0.0	1.1453877030753714	0.0
15	8.001590716234387E-5	0.0	0.0	1.1854356646101245	0.0
16	8.001590716234387E-5	0.0	0.0	1.228804286292115	0.0
17	8.001590716234387E-5	0.0	0.0	1.2842953179092003	0.0
18	1.2002386074351581E-4	0.0	0.0	1.3529889742080725	0.0
19	1.2002386074351581E-4	0.0	0.0	1.3986780571977708	0.0
20	1.2002386074351581E-4	0.0	0.0	1.453849025186207	0.0
21	1.2002386074351581E-4	0.0	0.0	1.514821146443913	0.0
22	1.2002386074351581E-4	0.0	0.0	1.5844749936287335	0.0
23	1.2002386074351581E-4	0.0	0.0	1.6612102485974212	0.0
24	1.2002386074351581E-4	0.0	0.0	1.7227024732516825	0.0
25	1.2002386074351581E-4	0.0	0.0	1.7989576327773962	0.0
26	1.2002386074351581E-4	0.0	0.0	1.851047988340082	0.0
27	1.2002386074351581E-4	0.0	0.0	1.9113799823404893	0.0
28	1.2002386074351581E-4	0.0	0.0	1.972712175180426	0.0
29	1.2002386074351581E-4	0.0	0.0	2.039485449707402	0.0
30	1.2002386074351581E-4	0.0	0.0	2.122982048831308	0.0
31	1.2002386074351581E-4	0.0	0.0	2.1964766595599206	0.0
32	1.2002386074351581E-4	0.0	0.0	2.2827338074809274	0.0
33	1.2002386074351581E-4	0.0	0.0	2.349467074054322	0.0
34	1.2002386074351581E-4	0.0	0.0	2.448606783028466	0.0
35	1.2002386074351581E-4	0.0	0.0	2.535864129789002	0.0
36	1.2002386074351581E-4	0.0	0.0	2.6147998222046542	0.0
37	1.2002386074351581E-4	0.0	0.0	2.705057765483778	0.0
38	1.2002386074351581E-4	0.0	0.0	2.8001166631926426	0.0
39	1.2002386074351581E-4	0.0	0.0	2.93146277479963	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	85	0.0	45.0	1
CGACGGT	395	0.0	43.86076	28
CGTTTTT	20280	0.0	43.768494	1
CGGTCTA	410	0.0	42.804874	31
CACGACG	420	0.0	40.714287	26
GCGCGAC	90	0.0	40.0	9
TCACGAC	425	0.0	39.70588	25
CTCAACT	2550	0.0	39.617645	2
ACGGTCT	450	0.0	39.5	30
ACGGGAC	885	0.0	39.152542	5
GCATTAG	2505	0.0	39.071857	16
GACGGTC	455	0.0	39.065933	29
ACTTGAC	2565	0.0	39.035088	6
CGCATCG	75	0.0	39.0	21
ACGGGTA	110	0.0	38.863636	5
TTGACCC	2565	0.0	38.596493	8
CTATGCG	105	0.0	38.57143	1
CCGCTCG	105	0.0	38.57143	19
AGGGATT	5510	0.0	38.384758	6
GCTCAAC	2665	0.0	38.24578	1
>>END_MODULE