FastQCFastQC Report
Thu 26 May 2016
SRR1547710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547710_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1467707
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64640.44041487844644744No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39530.26933168541132524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA207.033927E-445.022
TACCCGT253.8913357E-545.036
CGTATTA207.033927E-445.044
CGAGTTA207.033927E-445.014
CGTGATA207.033927E-445.013
TTACTCG207.033927E-445.023
CACGAGA207.033927E-445.016
CACGACT406.8175723E-945.026
CTTATCG207.033927E-445.041
CTACGCG351.2122291E-745.01
CGAGGTA253.8913357E-545.042
CATACGT207.033927E-445.042
GTATCGA253.8913357E-545.026
ACTCGAA207.033927E-445.025
TCGTAAT253.8913357E-545.040
ACGTCGT207.033927E-445.027
ACGTCGA253.8913357E-545.033
GCGGACC253.8913357E-545.05
GACGCAA406.8175723E-945.01
CACGTAA453.8562575E-1045.034