FastQCFastQC Report
Thu 26 May 2016
SRR1547709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547709_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1886443
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95110.5041763785070633No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33410.177105801765545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTA302.1662454E-645.00000444
CGCATCG600.045.00000421
CCGTATC302.1662454E-645.00000431
CCGCGAA207.034438E-445.00000419
CGCACCT207.034438E-445.00000436
CACGTCG207.034438E-445.00000431
ATACGAT207.034438E-445.00000435
AGTCGGT207.034438E-445.00000428
CCGGGTA253.8917613E-545.05
GTCGAAC453.8562575E-1045.021
CACGACG3800.044.40789426
CGACGGT4000.043.312528
CGGTCTA3950.043.29113831
TACGTAG1250.043.21
TTCGTAG1150.043.043481
TATAGCG1100.042.9545441
GTAAGCG1000.042.751
TACGGGA6550.042.5954174
CGTTTTT60650.042.5515251
ACGGTCT4100.042.25609630