##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547709_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1886443
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66778482042659	31.0	31.0	33.0	30.0	34.0
2	32.048279221794665	33.0	31.0	34.0	30.0	34.0
3	32.104161111679495	33.0	31.0	34.0	30.0	34.0
4	35.798801766075094	37.0	35.0	37.0	35.0	37.0
5	35.72286202127496	37.0	35.0	37.0	33.0	37.0
6	35.78964644041723	37.0	35.0	37.0	35.0	37.0
7	36.15305471726418	37.0	35.0	37.0	35.0	37.0
8	36.05276332229492	37.0	35.0	37.0	35.0	37.0
9	37.86959107696337	39.0	38.0	39.0	35.0	39.0
10	37.48375275584791	39.0	37.0	39.0	35.0	39.0
11	37.1156175935345	39.0	37.0	39.0	33.0	39.0
12	36.439725451550885	38.0	35.0	39.0	33.0	39.0
13	36.20503614474437	38.0	35.0	39.0	32.0	39.0
14	37.184560572463624	39.0	35.0	41.0	32.0	41.0
15	37.410732261722195	39.0	35.0	41.0	33.0	41.0
16	37.54863942350763	39.0	35.0	41.0	33.0	41.0
17	37.452465301098414	39.0	35.0	41.0	33.0	41.0
18	37.35647141207023	39.0	35.0	41.0	33.0	41.0
19	37.343321266531774	39.0	35.0	41.0	33.0	41.0
20	37.24365485731612	39.0	35.0	41.0	33.0	41.0
21	37.07916433202593	39.0	35.0	41.0	32.0	41.0
22	37.032673131390666	39.0	35.0	41.0	32.0	41.0
23	36.98112744461402	38.0	35.0	41.0	32.0	41.0
24	36.97253561332094	38.0	35.0	41.0	32.0	41.0
25	36.89115494080659	38.0	35.0	41.0	32.0	41.0
26	36.85833232172931	38.0	35.0	41.0	32.0	41.0
27	36.76413758592229	38.0	35.0	41.0	32.0	41.0
28	36.775292441913166	38.0	35.0	41.0	32.0	41.0
29	36.74098925862059	38.0	35.0	41.0	32.0	41.0
30	36.751036739514525	38.0	35.0	41.0	32.0	41.0
31	36.62327194619716	38.0	35.0	41.0	31.0	41.0
32	36.421709534822945	38.0	35.0	41.0	31.0	41.0
33	36.34321153620862	38.0	35.0	41.0	31.0	41.0
34	36.15906019953956	38.0	35.0	41.0	30.0	41.0
35	36.07268123129085	38.0	35.0	41.0	30.0	41.0
36	36.00519655245348	38.0	35.0	41.0	30.0	41.0
37	35.741560704458074	38.0	35.0	41.0	29.0	41.0
38	35.862601202368694	38.0	35.0	40.0	30.0	41.0
39	35.81576331752404	38.0	35.0	40.0	30.0	41.0
40	35.70478514325638	38.0	35.0	40.0	29.0	41.0
41	35.66126938370255	38.0	35.0	40.0	29.0	41.0
42	35.688671218796436	38.0	35.0	40.0	29.0	41.0
43	35.59397501011162	38.0	35.0	40.0	29.0	41.0
44	35.465608025262355	38.0	34.0	40.0	29.0	41.0
45	35.430831994393685	38.0	34.0	40.0	29.0	41.0
46	35.26600167617045	37.0	34.0	40.0	28.0	41.0
47	35.169208399087594	37.0	34.0	40.0	27.0	41.0
48	35.05768687418597	37.0	34.0	40.0	27.0	41.0
49	35.141809744582794	37.0	34.0	40.0	28.0	41.0
50	35.01306161914248	37.0	34.0	40.0	27.0	41.0
51	34.3964747410868	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	9.0
13	11.0
14	12.0
15	45.0
16	101.0
17	242.0
18	561.0
19	1064.0
20	2032.0
21	3333.0
22	5208.0
23	7786.0
24	11627.0
25	17075.0
26	23453.0
27	27776.0
28	29865.0
29	33290.0
30	38886.0
31	47692.0
32	61227.0
33	82440.0
34	153036.0
35	267300.0
36	115358.0
37	152329.0
38	254779.0
39	549763.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.90088436279283	23.961550918845678	30.548921965837295	13.588642752524194
2	30.828760794786803	25.249636485173422	30.7763870946538	13.145215625385978
3	29.16398746211786	25.36657614356755	32.130098815601634	13.339337578712954
4	26.543076043113945	26.872638081299037	31.580917101656397	15.00336877393062
5	23.413588430713254	32.739764731825986	29.823164548305993	14.023482289154773
6	22.366220447689116	40.60626268591206	26.415322381858342	10.612194484540481
7	89.07997750263327	3.451045168075579	6.134826231166275	1.3341510981248836
8	90.57686874185968	2.4027760181463207	5.601706492059395	1.418648747934605
9	87.02770240076164	4.5999269524708675	6.493013571043493	1.8793570757239948
10	48.24715085480982	33.75649304007595	10.662447791955548	7.3339083131586795
11	37.47354147461651	22.59257237032871	25.179769545117452	14.754116609937325
12	35.70661822276104	22.287924946579356	26.26901528432081	15.736441546338797
13	24.362305142535448	33.50437834591345	26.417283745122432	15.71603276642867
14	15.527900922529861	38.386370539687654	28.71239682301559	17.37333171476689
15	14.583478005961483	28.06853957421454	42.49145084161037	14.8565315782136
16	19.307182883341824	24.212393377377424	40.398888278097985	16.081535461182767
17	19.511641751168735	23.537366355622723	28.614434679446983	28.33655721376156
18	21.464523444387133	26.24070804153637	33.27362660838414	19.021141905692353
19	26.11926254861663	26.896704538647604	28.707678949218185	18.276353963517582
20	28.582840827949745	26.41500432295065	27.12740326635896	17.87475158274064
21	24.126093393757458	26.88345208416051	30.86989641351475	18.120558108567288
22	22.64330276610531	23.20653208180687	30.048085205860975	24.10207994622684
23	18.70573348889948	28.178640966093333	29.942436638689852	23.17318890631734
24	19.03169085946408	26.25099194621836	36.67637983230874	18.04093736200882
25	17.837909759266513	27.927533458471842	34.507059052407094	19.727497729854544
26	17.712382510364744	34.34935484401066	28.05258361901208	19.885679026612518
27	17.556427625960605	33.73300969072482	31.788821607649954	16.921741075664624
28	15.168229307750089	30.282759669918462	37.47651002442162	17.072500997909824
29	16.66093277135858	26.712813480184668	37.329513799250755	19.296739949205993
30	18.133757553236435	28.89326632185547	34.14876569289398	18.824210432014112
31	25.99134985790718	27.127880354720496	27.915977318159097	18.964792469213222
32	26.203707188608398	27.972326754638228	29.076574272320975	16.747391784432395
33	25.616570445012123	27.552754045576783	27.66985273342476	19.16082277598634
34	20.193135970713136	29.51098973040797	29.111348712895115	21.184525585983778
35	19.167873081773475	28.436639750048105	30.55549518326289	21.839991984915528
36	26.379593764561132	25.834334777144075	29.570148687238362	18.215922771056427
37	19.936356412571172	31.66244620166101	30.145358221796258	18.25583916397156
38	18.838152014134536	32.663165544890575	26.920028858544892	21.57865358243
39	19.312695904408457	30.936158685950225	29.43327733729564	20.31786807234568
40	22.51878270374456	27.929865890461574	27.91359187635142	21.637759529442448
41	18.46093414961385	25.928639243274247	29.661696642835217	25.948729964276684
42	21.110364850673992	26.562477636483052	27.728587611711564	24.59856990113139
43	21.52749910810981	26.831025374209556	27.460198903438904	24.181276614241725
44	19.686521140580446	30.152832606126978	29.500334757000346	20.660311496292227
45	18.549195496497905	34.19154461597832	26.42247870728138	20.83678118024239
46	21.692359641929283	31.255277789999486	27.532716334392294	19.519646233678937
47	20.745021185373744	29.507915160966963	27.655116004035108	22.091947649624185
48	21.725649807600867	27.284736406029758	30.605059363044628	20.384554423324744
49	21.19003860705041	25.644559628888867	31.562416675192413	21.60298508886831
50	19.362472123461984	31.102238445582508	29.437624142367408	20.0976652885881
51	19.04186874451017	32.69910620145957	26.76635339631253	21.492671657717725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1291.0
1	2194.5
2	3098.0
3	9734.0
4	16370.0
5	11705.0
6	7040.0
7	7340.0
8	7640.0
9	8047.0
10	8454.0
11	8449.0
12	8444.0
13	8275.5
14	8107.0
15	7809.0
16	7511.0
17	7063.0
18	6615.0
19	6456.5
20	6298.0
21	7435.0
22	8572.0
23	9517.0
24	10462.0
25	11851.5
26	17938.0
27	22635.0
28	26421.0
29	30207.0
30	35645.5
31	41084.0
32	47647.5
33	54211.0
34	61785.5
35	69360.0
36	70950.5
37	72541.0
38	86013.0
39	99485.0
40	117984.5
41	136484.0
42	160784.0
43	185084.0
44	186370.0
45	187656.0
46	178544.5
47	169433.0
48	161643.0
49	153853.0
50	145052.5
51	136252.0
52	119831.0
53	103410.0
54	93249.5
55	83089.0
56	74969.0
57	66849.0
58	59631.0
59	52413.0
60	46630.5
61	40848.0
62	36311.5
63	31775.0
64	24203.0
65	16631.0
66	13177.0
67	9723.0
68	7451.5
69	5180.0
70	3734.0
71	2288.0
72	1846.5
73	1405.0
74	1122.5
75	548.0
76	256.0
77	214.5
78	173.0
79	107.5
80	42.0
81	54.0
82	66.0
83	38.5
84	11.0
85	10.0
86	9.0
87	5.5
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1886443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.980379503180238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6601965617571	14.682813244846868
2	6.825554772326159	2.4545213025233465
3	2.047059464447986	1.1042071810905507
4	1.0298617329609723	0.7406921917776458
5	0.6165154706570977	0.5542591065998198
6	0.4491106357501344	0.4845107801821177
7	0.35374482127955	0.4452326293723684
8	0.29415226525324045	0.42311754887787206
9	0.2214139087486771	0.35829954959253607
>10	3.3100457715422906	14.952790377586808
>50	1.6302283888112126	21.59746863760012
>100	1.5550089478958737	40.734298952805986
>500	0.005922715474736689	0.6681882699941903
>1k	8.884073212105034E-4	0.2931743258098663
>5k	2.961357737368344E-4	0.506425901339927
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9511	0.5041763785070633	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3341	0.177105801765545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.3009817948382217E-5	0.0	0.0	0.020726838817817447	0.0
2	5.3009817948382217E-5	0.0	0.0	0.0694428615123807	0.0
3	5.3009817948382217E-5	0.0	0.0	0.09907534974552637	0.0
4	5.3009817948382217E-5	0.0	0.0	0.14556496008625758	0.0
5	5.3009817948382217E-5	0.0	0.0	0.2758630926033811	0.0
6	5.3009817948382217E-5	0.0	0.0	0.38850895574369326	0.0
7	5.3009817948382217E-5	0.0	0.0	0.4749679688175047	0.0
8	5.3009817948382217E-5	0.0	0.0	0.6580638800112169	0.0
9	5.3009817948382217E-5	0.0	0.0	0.7192372099236499	0.0
10	5.3009817948382217E-5	0.0	0.0	0.8381382315818713	0.0
11	5.3009817948382217E-5	0.0	0.0	1.0327902830883309	0.0
12	5.3009817948382217E-5	0.0	0.0	1.192455854748858	0.0
13	5.3009817948382217E-5	0.0	0.0	1.2490173304997818	0.0
14	5.3009817948382217E-5	0.0	0.0	1.2710164049483605	0.0
15	5.3009817948382217E-5	0.0	0.0	1.3089714345994021	0.0
16	5.3009817948382217E-5	0.0	0.0	1.3849875135373824	0.0
17	5.3009817948382217E-5	0.0	0.0	1.493392591241824	0.0
18	5.3009817948382217E-5	0.0	0.0	1.6283025779204567	0.0
19	5.3009817948382217E-5	0.0	0.0	1.7160868364429775	0.0
20	5.3009817948382217E-5	0.0	0.0	1.8070516840424016	0.0
21	5.3009817948382217E-5	0.0	0.0	1.926270764608313	0.0
22	5.3009817948382217E-5	0.0	0.0	2.0556147204023656	0.0
23	5.3009817948382217E-5	0.0	0.0	2.1872380983681987	0.0
24	5.3009817948382217E-5	0.0	0.0	2.2879037426521767	0.0
25	5.3009817948382217E-5	0.0	0.0	2.3733025593670205	0.0
26	5.3009817948382217E-5	0.0	0.0	2.4573761306331545	5.3009817948382217E-5
27	5.3009817948382217E-5	0.0	0.0	2.5464326247864366	5.3009817948382217E-5
28	5.3009817948382217E-5	0.0	0.0	2.6391998061961055	5.3009817948382217E-5
29	5.3009817948382217E-5	0.0	0.0	2.7476578937184954	5.3009817948382217E-5
30	5.3009817948382217E-5	0.0	0.0	2.884211184753528	5.3009817948382217E-5
31	5.3009817948382217E-5	0.0	0.0	3.008572217660433	5.3009817948382217E-5
32	5.3009817948382217E-5	0.0	0.0	3.1189386586289647	5.3009817948382217E-5
33	5.3009817948382217E-5	0.0	0.0	3.2314785021333803	5.3009817948382217E-5
34	5.3009817948382217E-5	0.0	0.0	3.3539841914120916	5.3009817948382217E-5
35	5.3009817948382217E-5	0.0	0.0	3.5069705260111226	5.3009817948382217E-5
36	5.3009817948382217E-5	0.0	0.0	3.629794274197524	5.3009817948382217E-5
37	5.3009817948382217E-5	0.0	0.0	3.7529890911095642	5.3009817948382217E-5
38	5.3009817948382217E-5	0.0	0.0	3.896751717385577	5.3009817948382217E-5
39	5.3009817948382217E-5	0.0	0.0	4.101316604848384	5.3009817948382217E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTA	30	2.1662454E-6	45.000004	44
CGCATCG	60	0.0	45.000004	21
CCGTATC	30	2.1662454E-6	45.000004	31
CCGCGAA	20	7.034438E-4	45.000004	19
CGCACCT	20	7.034438E-4	45.000004	36
CACGTCG	20	7.034438E-4	45.000004	31
ATACGAT	20	7.034438E-4	45.000004	35
AGTCGGT	20	7.034438E-4	45.000004	28
CCGGGTA	25	3.8917613E-5	45.0	5
GTCGAAC	45	3.8562575E-10	45.0	21
CACGACG	380	0.0	44.407894	26
CGACGGT	400	0.0	43.3125	28
CGGTCTA	395	0.0	43.291138	31
TACGTAG	125	0.0	43.2	1
TTCGTAG	115	0.0	43.04348	1
TATAGCG	110	0.0	42.954544	1
GTAAGCG	100	0.0	42.75	1
TACGGGA	655	0.0	42.595417	4
CGTTTTT	6065	0.0	42.551525	1
ACGGTCT	410	0.0	42.256096	30
>>END_MODULE