FastQCFastQC Report
Thu 26 May 2016
SRR1547708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547708_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2225608
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158400.7117156300660313No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57530.25849116286426005No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG48420.2175585278270028No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC32550.14625217019349318No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC23960.10765597535594768No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTA207.0347113E-445.00000420
TCCGAAC207.0347113E-445.00000415
CGCGCCA406.8193913E-945.00000437
CATTCGG207.0347113E-445.00000428
ATCCGAT207.0347113E-445.00000418
TACGCTA207.0347113E-445.00000410
TCGGACG207.0347113E-445.0000041
CCGTAAG207.0347113E-445.0000041
TACGATA207.0347113E-445.00000416
GAACCGT207.0347113E-445.00000416
CGCACCG406.8193913E-945.00000438
GCTACGC207.0347113E-445.00000420
CGTTTCG406.8193913E-945.00000441
CTCTACG207.0347113E-445.00000427
AGTCCGC253.8919876E-545.010
GCGAAGT351.2126111E-745.021
TAGGTCG551.8189894E-1245.01
CACGCGT253.8919876E-545.027
CCGGACG253.8919876E-545.037
ATGTCGC253.8919876E-545.010