FastQCFastQC Report
Thu 26 May 2016
SRR1547707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547707_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1169910
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58860.5031156242787908No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47680.40755271773042373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA302.165536E-645.0000048
CTCACCG207.0333376E-445.0000041
CGAACTG253.8908478E-545.00000417
GGTCGAC207.0333376E-445.0000048
ACGTTAG502.1827873E-1145.0000041
GGCACGT207.0333376E-445.0000048
CTATACG302.165536E-645.0000041
TAGCGGC207.0333376E-445.00000421
TCCTCGA207.0333376E-445.00000420
CGCGATA207.0333376E-445.00000423
CTAAGCG502.1827873E-1145.0000041
TAGCCCG207.0333376E-445.0000041
CGTAGCG207.0333376E-445.0000042
GCGGATA207.0333376E-445.00000421
ACGTAAC253.8908478E-545.00000432
ATACGGT253.8908478E-545.0000043
TTCGCGC207.0333376E-445.00000415
CGTCCCA253.8908478E-545.00000428
CAATCGT207.0333376E-445.00000411
TCGGTGA207.0333376E-445.00000424