##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547707_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1169910
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.635066800010257	31.0	31.0	33.0	30.0	34.0
2	32.0035891649785	33.0	31.0	34.0	30.0	34.0
3	32.03532322999205	33.0	31.0	34.0	30.0	34.0
4	35.740042396423654	37.0	35.0	37.0	35.0	37.0
5	35.67262182561052	37.0	35.0	37.0	33.0	37.0
6	35.743802514723356	37.0	35.0	37.0	33.0	37.0
7	36.12983391884846	37.0	35.0	37.0	35.0	37.0
8	36.04326828559462	37.0	35.0	37.0	35.0	37.0
9	37.887693070407124	39.0	38.0	39.0	35.0	39.0
10	37.397826328521	39.0	37.0	39.0	35.0	39.0
11	37.05899770067783	39.0	37.0	39.0	33.0	39.0
12	35.94584711644485	37.0	35.0	39.0	33.0	39.0
13	35.49888965817883	37.0	35.0	39.0	31.0	39.0
14	36.300192322486346	38.0	35.0	41.0	31.0	41.0
15	36.648699472609	38.0	35.0	41.0	32.0	41.0
16	36.85990033421374	38.0	35.0	41.0	33.0	41.0
17	36.817110717918474	38.0	35.0	41.0	33.0	41.0
18	36.7611089741946	37.0	35.0	40.0	32.0	41.0
19	36.69693737125078	37.0	35.0	40.0	32.0	41.0
20	36.5418647588276	36.0	35.0	40.0	32.0	41.0
21	36.35836004478977	36.0	35.0	40.0	32.0	41.0
22	36.29924951491995	36.0	35.0	40.0	32.0	41.0
23	36.27418861279928	36.0	35.0	40.0	32.0	41.0
24	36.25685907462967	36.0	35.0	40.0	32.0	41.0
25	36.175178432528995	36.0	35.0	40.0	32.0	41.0
26	36.15084835585643	36.0	35.0	40.0	32.0	41.0
27	36.04175791300185	36.0	35.0	40.0	31.0	41.0
28	36.09934952261285	36.0	35.0	40.0	31.0	41.0
29	36.09180962638152	36.0	35.0	40.0	31.0	41.0
30	36.09877170038721	36.0	35.0	40.0	31.0	41.0
31	35.901459086596404	36.0	35.0	40.0	31.0	41.0
32	35.64736005333744	36.0	35.0	40.0	30.0	41.0
33	35.54201690728347	36.0	35.0	40.0	30.0	41.0
34	35.37809489618859	36.0	35.0	40.0	30.0	41.0
35	35.2741920318657	35.0	34.0	40.0	30.0	41.0
36	35.13942269063432	35.0	34.0	40.0	29.0	41.0
37	34.86790265917891	35.0	34.0	40.0	27.0	41.0
38	35.01705857715551	35.0	34.0	40.0	29.0	41.0
39	35.006653503260935	36.0	34.0	40.0	28.0	41.0
40	34.844809429785194	36.0	34.0	40.0	27.0	41.0
41	34.861009821268304	36.0	34.0	40.0	28.0	41.0
42	34.85929686898992	36.0	34.0	40.0	28.0	41.0
43	34.75192279748015	35.0	34.0	40.0	27.0	41.0
44	34.623906112435996	35.0	34.0	40.0	27.0	41.0
45	34.603150669709635	35.0	34.0	40.0	27.0	41.0
46	34.424740364643434	35.0	34.0	40.0	26.0	41.0
47	34.324434358198495	35.0	34.0	40.0	26.0	41.0
48	34.229549281568666	35.0	34.0	40.0	26.0	41.0
49	34.33575745142789	35.0	34.0	40.0	26.0	41.0
50	34.18961287620415	35.0	34.0	39.0	26.0	41.0
51	33.532525578890684	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	5.0
15	28.0
16	79.0
17	168.0
18	425.0
19	812.0
20	1522.0
21	2593.0
22	4025.0
23	5883.0
24	8801.0
25	12949.0
26	17704.0
27	20253.0
28	21166.0
29	23133.0
30	26803.0
31	33797.0
32	42841.0
33	58398.0
34	117843.0
35	237499.0
36	62902.0
37	79978.0
38	129353.0
39	260855.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.136848133617118	24.5326563581814	31.167183800463285	14.163311707738202
2	30.01777914540435	24.692241283517536	32.825345539400466	12.464634031677651
3	28.668273627885902	24.766264071595252	33.16126881554992	13.404193484968928
4	25.21014437007975	27.683240591156583	32.96586916942329	14.140745869340376
5	22.865092186578455	31.794069629287726	31.586788727338	13.754049456795823
6	21.40147532716192	41.409082749955125	27.352531391303604	9.836910531579353
7	88.55758135241173	3.776273388551256	6.45639408159602	1.2097511774409997
8	90.58089938542281	2.6464428887692217	5.624449743997402	1.1482079818105666
9	87.79854860630306	3.7035327503825077	6.76120385328786	1.7367147900265834
10	54.400765870878956	19.22968433469241	17.02977152088622	9.339778273542409
11	49.86298091306169	18.08241659614842	18.535100990674497	13.519501500115394
12	46.457419801523194	20.430716892752436	21.277363215973878	11.834500089750494
13	23.990306946688207	42.16760263610021	20.859980682274703	12.982109734936875
14	14.331187869152329	46.52631398996504	25.18792043832432	13.954577702558316
15	13.163320255404262	25.34400082057594	49.28652631398997	12.206152610029832
16	15.529998034036808	19.383798753750288	49.569710490550555	15.51649272166235
17	16.5552905779077	18.79606123547965	27.01130856219709	37.63733962441555
18	21.30249335418964	24.359651597131403	34.966877794018345	19.370977254660616
19	28.449624330076674	25.92096827961125	25.91079655700011	19.718610833311963
20	31.740732193074685	23.738663657888214	25.003803711396603	19.5168004376405
21	22.11050422682087	27.918814267764187	30.216597857954884	19.75408364746006
22	21.99451239838962	22.182048191741245	28.001470198562284	27.82196921130685
23	16.92617380824166	30.416527767093193	26.073629595438963	26.58366882922618
24	18.22815430246771	24.697797266456394	39.72809874263832	17.345949688437575
25	15.49768785633083	24.95952680120693	38.13370259250712	21.409082749955125
26	16.1469685702319	36.164662238975644	26.44075185270662	21.247617338085835
27	16.245694113222385	37.7770084878324	29.951107350138045	16.026190048807173
28	12.49173013308716	30.421570890068466	41.01623201784753	16.070466958996846
29	14.065013548050706	24.64975938320042	40.596456137651614	20.688770931097263
30	16.671367882999547	32.33009376789668	34.47179697583575	16.526741373268028
31	30.432169995982594	26.80744672667128	25.618295424434358	17.142087852911764
32	30.592438734603515	27.528442358813926	26.844970980673725	15.034147925908831
33	28.324571975622053	28.83161952628835	24.696002256583842	18.147806241505755
34	19.27806412459078	29.412177005068767	26.778128232086228	24.531630638254224
35	17.871374721132394	27.939841526271252	31.126155003376326	23.062628749220025
36	31.945790701848857	23.274696344163225	28.774093733706014	16.0054192202819
37	19.044029027873936	32.214614799429015	31.34070142147686	17.40065475122018
38	18.65357164226308	33.85405714969528	24.29468933507706	23.197681872964587
39	18.815378960774762	32.58173705669667	28.88529886914378	19.71758511338479
40	23.57865134924909	27.030882717473993	25.661717568018055	23.728748365258866
41	17.65195613337778	23.942781923395817	28.835722405997043	29.56953953722936
42	22.31581916557684	23.539332085374088	25.93216572214957	28.212683026899505
43	23.03724218102247	25.330580984862085	25.700951355232455	25.93122547888299
44	18.89170961868862	30.730739971450795	29.45713772854322	20.920412681317366
45	16.966091408740844	40.62756964210922	22.184099631595593	20.22223931755434
46	23.312904411450454	34.015522561564566	25.5499995726167	17.121573454368285
47	22.14332726449043	29.67441939978289	25.020129753570785	23.162123582155893
48	23.447701105213223	26.66726500329085	29.968202682257612	19.916831209238318
49	21.838688446119786	23.027583318374916	32.37180637826842	22.76192185723688
50	20.316092690890752	33.00672701318905	27.555623936884032	19.121556359036166
51	17.745125693429408	35.94695318443299	24.10877759827679	22.19914352386081
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1174.0
1	1467.0
2	1760.0
3	5413.0
4	9066.0
5	6539.5
6	4013.0
7	4521.0
8	5029.0
9	5653.0
10	6277.0
11	6507.0
12	6737.0
13	6634.5
14	6532.0
15	6036.5
16	5541.0
17	5103.0
18	4665.0
19	4445.0
20	4225.0
21	4307.0
22	4389.0
23	4538.0
24	4687.0
25	5341.0
26	5987.5
27	5980.0
28	8275.0
29	10570.0
30	12342.5
31	14115.0
32	16381.5
33	18648.0
34	21952.5
35	25257.0
36	28379.0
37	31501.0
38	38595.5
39	45690.0
40	65692.5
41	85695.0
42	113936.0
43	142177.0
44	143610.5
45	145044.0
46	136502.0
47	127960.0
48	118861.0
49	109762.0
50	98227.0
51	86692.0
52	79852.5
53	73013.0
54	60894.5
55	48776.0
56	44553.0
57	40330.0
58	36945.5
59	33561.0
60	27944.5
61	22328.0
62	18506.0
63	14684.0
64	11480.0
65	8276.0
66	6483.5
67	4691.0
68	3483.5
69	2276.0
70	1727.0
71	1178.0
72	1022.0
73	866.0
74	553.5
75	274.0
76	307.0
77	204.0
78	101.0
79	69.0
80	37.0
81	44.0
82	51.0
83	28.5
84	6.0
85	4.0
86	2.0
87	2.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1169910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.06247230395724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.40491327654799	15.517789047402369
2	7.047525217825277	2.686865085524691
3	2.1677665260933057	1.2396896809529772
4	1.136921382544972	0.866901294661612
5	0.6940893798697779	0.6615529790119249
6	0.48035993543231276	0.549410877906549
7	0.34350822995255176	0.4583681283756325
8	0.2977014290640243	0.4539940197105164
9	0.2546279836588382	0.43684549976781717
>10	3.263898957929351	15.049160335350509
>50	1.5007782533074296	20.99810402729645
>100	1.4020487737360487	39.44249375536069
>500	0.004508195413942279	0.6294051530047011
>1k	9.01639082788456E-4	0.5035928722839361
>5k	4.50819541394228E-4	0.5058272433896328
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5886	0.5031156242787908	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4768	0.40755271773042373	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.547666059782376E-5	0.0	0.0	0.03410518757853168	0.0
2	8.547666059782376E-5	0.0	0.0	0.11753040832200767	0.0
3	8.547666059782376E-5	0.0	0.0	0.17001307792907147	0.0
4	8.547666059782376E-5	0.0	0.0	0.2520706721029823	0.0
5	8.547666059782376E-5	0.0	0.0	0.44302553187852056	0.0
6	8.547666059782376E-5	0.0	0.0	0.6147481430195485	0.0
7	8.547666059782376E-5	0.0	0.0	0.7310818780931867	0.0
8	8.547666059782376E-5	0.0	0.0	0.9894778230804079	0.0
9	8.547666059782376E-5	0.0	0.0	1.068543734133395	0.0
10	8.547666059782376E-5	0.0	0.0	1.2422323084681728	0.0
11	8.547666059782376E-5	0.0	0.0	1.5296048413980563	0.0
12	8.547666059782376E-5	0.0	0.0	1.733466676923866	0.0
13	8.547666059782376E-5	0.0	0.0	1.8057799317896248	0.0
14	8.547666059782376E-5	1.7095332119564753E-4	0.0	1.8351839030352761	0.0
15	8.547666059782376E-5	1.7095332119564753E-4	0.0	1.88963253583609	0.0
16	8.547666059782376E-5	1.7095332119564753E-4	0.0	2.034259045567608	0.0
17	8.547666059782376E-5	1.7095332119564753E-4	0.0	2.195553504115701	0.0
18	8.547666059782376E-5	1.7095332119564753E-4	0.0	2.3923207768118915	0.0
19	8.547666059782376E-5	1.7095332119564753E-4	0.0	2.5020728090194972	0.0
20	8.547666059782376E-5	1.7095332119564753E-4	0.0	2.613705327760255	0.0
21	8.547666059782376E-5	1.7095332119564753E-4	0.0	2.770725953278457	0.0
22	8.547666059782376E-5	1.7095332119564753E-4	0.0	2.933900898359703	0.0
23	8.547666059782376E-5	1.7095332119564753E-4	0.0	3.118701438572198	0.0
24	8.547666059782376E-5	1.7095332119564753E-4	0.0	3.2448649896145856	0.0
25	8.547666059782376E-5	1.7095332119564753E-4	0.0	3.357865134924909	0.0
26	8.547666059782376E-5	1.7095332119564753E-4	0.0	3.4735150567137643	0.0
27	8.547666059782376E-5	1.7095332119564753E-4	0.0	3.586173295381696	0.0
28	8.547666059782376E-5	1.7095332119564753E-4	0.0	3.703105367079519	0.0
29	8.547666059782376E-5	1.7095332119564753E-4	0.0	3.833115367848809	0.0
30	8.547666059782376E-5	1.7095332119564753E-4	0.0	3.9951791163422827	0.0
31	8.547666059782376E-5	1.7095332119564753E-4	0.0	4.1495499653819525	0.0
32	8.547666059782376E-5	1.7095332119564753E-4	0.0	4.266396560419177	0.0
33	8.547666059782376E-5	1.7095332119564753E-4	0.0	4.386918651862109	0.0
34	8.547666059782376E-5	1.7095332119564753E-4	0.0	4.523082972194442	0.0
35	8.547666059782376E-5	1.7095332119564753E-4	0.0	4.720277628193622	0.0
36	8.547666059782376E-5	1.7095332119564753E-4	0.0	4.869434400936824	0.0
37	8.547666059782376E-5	1.7095332119564753E-4	0.0	5.015941397201495	0.0
38	8.547666059782376E-5	1.7095332119564753E-4	0.0	5.172021779453121	0.0
39	8.547666059782376E-5	1.7095332119564753E-4	0.0	5.3495568035148	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	30	2.165536E-6	45.000004	8
CTCACCG	20	7.0333376E-4	45.000004	1
CGAACTG	25	3.8908478E-5	45.000004	17
GGTCGAC	20	7.0333376E-4	45.000004	8
ACGTTAG	50	2.1827873E-11	45.000004	1
GGCACGT	20	7.0333376E-4	45.000004	8
CTATACG	30	2.165536E-6	45.000004	1
TAGCGGC	20	7.0333376E-4	45.000004	21
TCCTCGA	20	7.0333376E-4	45.000004	20
CGCGATA	20	7.0333376E-4	45.000004	23
CTAAGCG	50	2.1827873E-11	45.000004	1
TAGCCCG	20	7.0333376E-4	45.000004	1
CGTAGCG	20	7.0333376E-4	45.000004	2
GCGGATA	20	7.0333376E-4	45.000004	21
ACGTAAC	25	3.8908478E-5	45.000004	32
ATACGGT	25	3.8908478E-5	45.000004	3
TTCGCGC	20	7.0333376E-4	45.000004	15
CGTCCCA	25	3.8908478E-5	45.000004	28
CAATCGT	20	7.0333376E-4	45.000004	11
TCGGTGA	20	7.0333376E-4	45.000004	24
>>END_MODULE