FastQCFastQC Report
Thu 26 May 2016
SRR1547706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547706_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1619402
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89420.5521791377310884No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21750.13430883745975367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA253.8915146E-545.020
ACTCGAT207.034143E-445.033
TATCGGA253.8915146E-545.044
CGGTCTA2600.043.2692331
CGACGGT2450.043.16326528
ACTACGG750.042.02
TACGGGA7500.041.74
TACGTAG556.184564E-1140.9090921
ACGTAGG1500.040.52
CGTTTTT55300.040.280291
CTCACGA2750.040.09090824
ACGGTCT2700.040.00000430
ATTACGT451.9292202E-840.021
TCGATTG451.9292202E-840.01
GCTACGG1700.039.7058832
CGTGCGG1200.039.3750042
ACGGGAT6800.039.044125
GATCGAC750.039.09
CCGGATA356.249875E-638.57142620
ATAGCCG356.249875E-638.5714261
Produced by FastQC (version 0.11.5)