##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547706_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1619402
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.615540180881585	31.0	31.0	33.0	30.0	34.0
2	31.996929730851264	33.0	31.0	34.0	30.0	34.0
3	32.058272745124434	33.0	31.0	34.0	30.0	34.0
4	35.767669794158586	37.0	35.0	37.0	35.0	37.0
5	35.6931404308504	37.0	35.0	37.0	33.0	37.0
6	35.74975268648551	37.0	35.0	37.0	33.0	37.0
7	36.095222804467326	37.0	35.0	37.0	35.0	37.0
8	35.989211449658576	37.0	35.0	37.0	35.0	37.0
9	37.777155394398676	39.0	38.0	39.0	35.0	39.0
10	37.40674705848208	39.0	37.0	39.0	35.0	39.0
11	37.097835497300856	39.0	37.0	39.0	33.0	39.0
12	36.42871380917153	38.0	35.0	39.0	33.0	39.0
13	36.1992253930772	38.0	35.0	39.0	32.0	39.0
14	37.13087979389923	39.0	35.0	41.0	32.0	41.0
15	37.355344133204724	39.0	35.0	41.0	32.0	41.0
16	37.463823683063254	39.0	35.0	41.0	33.0	41.0
17	37.37423320460269	39.0	35.0	41.0	33.0	41.0
18	37.295095967523814	39.0	35.0	41.0	32.0	41.0
19	37.25917591802406	39.0	35.0	41.0	32.0	41.0
20	37.15707897112638	39.0	35.0	41.0	32.0	41.0
21	37.024066291137096	39.0	35.0	41.0	32.0	41.0
22	36.99716685541947	39.0	35.0	41.0	32.0	41.0
23	36.90568308548464	38.0	35.0	41.0	32.0	41.0
24	36.89196876377824	38.0	35.0	41.0	32.0	41.0
25	36.81412089153898	38.0	35.0	41.0	32.0	41.0
26	36.775129337866694	38.0	35.0	41.0	32.0	41.0
27	36.67794778566409	38.0	35.0	41.0	31.0	41.0
28	36.68093036812354	38.0	35.0	41.0	31.0	41.0
29	36.60188514031723	38.0	35.0	41.0	31.0	41.0
30	36.59617747785911	38.0	35.0	41.0	31.0	41.0
31	36.450342780853674	38.0	35.0	41.0	31.0	41.0
32	36.279328418761985	38.0	35.0	41.0	30.0	41.0
33	36.19868815772736	38.0	35.0	41.0	30.0	41.0
34	36.020608224517446	38.0	35.0	41.0	30.0	41.0
35	35.922809160418474	38.0	35.0	41.0	30.0	41.0
36	35.83578197383972	38.0	35.0	41.0	29.0	41.0
37	35.58525245738859	38.0	35.0	40.0	28.0	41.0
38	35.69275139835569	38.0	35.0	40.0	29.0	41.0
39	35.61080756970783	38.0	35.0	40.0	29.0	41.0
40	35.49040571766615	38.0	35.0	40.0	28.0	41.0
41	35.436025767536414	38.0	35.0	40.0	28.0	41.0
42	35.44934858670052	38.0	35.0	40.0	28.0	41.0
43	35.36863731179781	38.0	34.0	40.0	28.0	41.0
44	35.21742285115123	37.0	34.0	40.0	27.0	41.0
45	35.206791148831485	37.0	34.0	40.0	27.0	41.0
46	35.06001412867219	37.0	34.0	40.0	27.0	41.0
47	34.94776899126962	37.0	34.0	40.0	26.0	41.0
48	34.82134207565509	37.0	34.0	40.0	26.0	41.0
49	34.899848833087766	37.0	34.0	40.0	27.0	41.0
50	34.76254012283547	36.0	34.0	40.0	26.0	41.0
51	34.181915299598245	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	3.0
12	6.0
13	7.0
14	16.0
15	61.0
16	128.0
17	319.0
18	649.0
19	1249.0
20	2275.0
21	3445.0
22	5399.0
23	7759.0
24	11264.0
25	16117.0
26	21531.0
27	25405.0
28	27746.0
29	30092.0
30	35414.0
31	42865.0
32	53926.0
33	72264.0
34	131312.0
35	213012.0
36	101993.0
37	136413.0
38	224078.0
39	454522.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.50415770759824	23.641936961915572	28.79661751683646	14.05728781364973
2	31.14143368972003	24.51176421913768	30.719734815691226	13.62706727545106
3	30.048190628392458	24.38338349588305	31.543927943771838	14.02449793195266
4	27.019912288610243	26.76988172177137	31.60487636794323	14.605329621675162
5	23.81187623579568	31.85614195857483	29.667309290713483	14.664672514915999
6	22.660278300261457	39.84767216540427	26.633535095053606	10.858514439280672
7	87.56485418691591	3.8992788696074228	6.803560820599208	1.7323061228774572
8	88.32118275758583	3.1955005613183136	6.642390215647505	1.840926465448357
9	84.19330098394346	5.201611459044758	7.824740243620794	2.780347313390992
10	45.83994585655692	32.05461028206709	13.071738827048504	9.033705034327486
11	38.79462912853016	20.66065127744686	25.0330060108608	15.511713583162178
12	35.660385747331425	20.640335136056397	28.068385737451234	15.630893379160948
13	25.94865265079332	30.67014861041298	26.69293973948408	16.68825899930962
14	17.441808766445885	34.66347454183705	30.339779745856806	17.554936945860263
15	17.12551917312687	27.008303064958543	40.7685676564559	15.097610105458681
16	22.373135268450948	23.816816330966617	37.98544153953126	15.824606861051176
17	21.92247508648254	23.30650449980919	28.362321400121775	26.408699013586496
18	23.347939548055393	25.630078263457744	32.67916181405235	18.342820374434513
19	27.098768557776264	26.672129588576528	27.670584573811812	18.558517279835396
20	28.787293087201327	26.6736116171278	27.03744962646705	17.501645669203818
21	25.447109488564294	26.65236920789279	30.111053339442584	17.78946796410033
22	23.895857853701553	22.617855232981064	30.866393891078314	22.619893022239072
23	20.780201580583448	26.77883564426869	30.78821688499829	21.652745890149575
24	21.013003565513692	25.724187076464027	35.5859138126296	17.676895545392682
25	20.50238297840808	26.582343358844806	32.64945949183711	20.26581417091
26	18.722281434751842	32.902392364588906	27.19942299688404	21.17590320377522
27	18.587478587774996	31.57295100290107	31.48884588261593	18.350724526708007
28	16.62842209655169	28.757961272123904	35.59733778271239	19.01627884861202
29	18.150280165147382	26.68157752059093	35.3876924938959	19.78044982036579
30	20.170593836490262	28.53454546801844	32.62951385758447	18.66534683790683
31	26.52664378579253	25.71492439801853	29.13600205507959	18.622429761109345
32	27.68960394021991	26.245614121756056	28.531087401398786	17.533694536625248
33	25.689853415026043	27.159408225999478	28.027691703480667	19.12304665549382
34	20.27180403630476	27.794951469740063	30.06220814843998	21.871036345515197
35	19.7454368958418	27.791369900741138	30.86361508754466	21.599578115872404
36	26.59191479323849	25.93377061409088	28.890108817946373	18.58420577472425
37	20.49812214632315	31.17595260472693	29.360591131788155	18.965334117161767
38	21.26741846681676	30.975261238407757	26.01095960113672	21.746360693638763
39	20.670654970168002	30.014597981230107	28.364667945327966	20.950079103273925
40	23.531711088414117	26.70356094410159	27.30674656447257	22.45798140301173
41	19.348129741719475	25.421544496054715	28.765247912501035	26.46507784972478
42	21.033998969990154	25.73468477870226	28.74067093902564	24.49064531228194
43	21.921116560310537	26.368066730805566	27.94852667836646	23.762290030517438
44	20.95261090204903	28.230544361437122	29.643596833893003	21.173247902620844
45	19.60328565729819	32.41974506638871	26.002931946483947	21.97403732982916
46	22.51466899509819	29.60463183323227	27.914687026445566	19.966012145223978
47	20.974532574370045	27.821627983663106	28.614266253839382	22.589573188127467
48	22.046471475272973	26.714305651098368	30.648906201177965	20.590316672450694
49	22.31231034665883	25.096300980238382	31.296305673328796	21.29508299977399
50	20.417722097416206	29.659466889629627	28.933334650692043	20.98947636226212
51	19.418526097905275	31.170147992901082	27.199855255211492	22.21147065398215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1273.0
1	2087.0
2	2901.0
3	9358.5
4	15816.0
5	11097.5
6	6379.0
7	6517.5
8	6656.0
9	6951.5
10	7247.0
11	7129.5
12	7012.0
13	6954.0
14	6896.0
15	6491.0
16	6086.0
17	6185.0
18	6284.0
19	6103.5
20	5923.0
21	6033.0
22	6143.0
23	7366.0
24	8589.0
25	10583.0
26	15879.5
27	19182.0
28	22766.5
29	26351.0
30	30563.5
31	34776.0
32	38288.0
33	41800.0
34	45690.5
35	49581.0
36	53699.0
37	57817.0
38	64272.0
39	70727.0
40	87126.0
41	103525.0
42	119782.5
43	136040.0
44	141661.5
45	147283.0
46	141006.0
47	134729.0
48	130110.5
49	125492.0
50	118171.0
51	110850.0
52	104728.0
53	98606.0
54	92090.0
55	85574.0
56	78404.5
57	71235.0
58	66689.5
59	62144.0
60	55488.0
61	48832.0
62	42392.0
63	35952.0
64	30002.5
65	24053.0
66	19265.5
67	14478.0
68	11698.0
69	8918.0
70	6851.0
71	4784.0
72	3878.5
73	2973.0
74	2444.0
75	1512.5
76	1110.0
77	800.0
78	490.0
79	336.0
80	182.0
81	125.0
82	68.0
83	92.0
84	116.0
85	68.0
86	20.0
87	14.5
88	9.0
89	5.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1619402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.56328035442291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.83153942866241	16.008933476769354
2	5.715453382272076	2.2362603374004664
3	1.8285742405060559	1.0731873154768776
4	0.9459750878250066	0.7402550340568177
5	0.6151487676849794	0.6017163900949511
6	0.4541893200067122	0.5331259800765608
7	0.3610717860642598	0.4944624005193129
8	0.30309169505152	0.47435742427121136
9	0.27438933472048666	0.48311599332604216
>10	4.476171461671515	22.53701657737176
>50	2.3809653016464765	33.482739372786924
>100	0.8096231883008422	20.214749897421317
>500	0.003172504656349009	0.4301076642638237
>1k	3.172504656349009E-4	0.13499050068886242
>5k	3.172504656349009E-4	0.5549816354757737
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8942	0.5521791377310884	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2175	0.13430883745975367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021427662803923917	0.0
2	0.0	0.0	0.0	0.06891432763452188	0.0
3	0.0	0.0	0.0	0.09491157847155926	0.0
4	0.0	0.0	0.0	0.1322710482017436	0.0
5	0.0	0.0	0.0	0.23446926705042972	0.0
6	0.0	0.0	0.0	0.32499651105778554	0.0
7	0.0	0.0	0.0	0.39699839817414084	0.0
8	0.0	0.0	0.0	0.528590183289881	0.0
9	0.0	0.0	0.0	0.5808316897225025	0.0
10	0.0	0.0	0.0	0.6782133157795285	0.0
11	0.0	0.0	0.0	0.8303064958546427	0.0
12	0.0	0.0	0.0	0.9567111810409028	0.0
13	0.0	0.0	0.0	1.0020365542342173	0.0
14	0.0	0.0	0.0	1.0236494706070511	0.0
15	0.0	0.0	0.0	1.0589093998895889	0.0
16	0.0	0.0	0.0	1.1291822536961174	0.0
17	0.0	0.0	0.0	1.2220560429096667	0.0
18	0.0	0.0	0.0	1.327959333136553	0.0
19	0.0	0.0	0.0	1.3965031536332548	0.0
20	0.0	0.0	0.0	1.4720866097485368	0.0
21	0.0	0.0	0.0	1.5650221501517227	0.0
22	0.0	0.0	0.0	1.6697521677755123	0.0
23	0.0	0.0	0.0	1.7741734294511184	0.0
24	0.0	0.0	0.0	1.854696980737334	0.0
25	0.0	0.0	0.0	1.9300334320940693	0.0
26	0.0	0.0	0.0	2.005122878692258	0.0
27	0.0	0.0	0.0	2.0918215489421406	0.0
28	0.0	0.0	0.0	2.169010535987976	0.0
29	0.0	0.0	0.0	2.267874190596282	0.0
30	0.0	0.0	0.0	2.389215278232335	0.0
31	0.0	0.0	0.0	2.499626405302698	0.0
32	0.0	0.0	0.0	2.590894663585694	0.0
33	0.0	0.0	0.0	2.68660900752253	0.0
34	0.0	0.0	0.0	2.7965261250757996	0.0
35	0.0	0.0	0.0	2.9306497089666435	0.0
36	0.0	0.0	0.0	3.039023046778996	0.0
37	0.0	0.0	0.0	3.158017589208856	0.0
38	0.0	0.0	0.0	3.2962167516157197	0.0
39	0.0	0.0	0.0	3.5556952504690003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	25	3.8915146E-5	45.0	20
ACTCGAT	20	7.034143E-4	45.0	33
TATCGGA	25	3.8915146E-5	45.0	44
CGGTCTA	260	0.0	43.26923	31
CGACGGT	245	0.0	43.163265	28
ACTACGG	75	0.0	42.0	2
TACGGGA	750	0.0	41.7	4
TACGTAG	55	6.184564E-11	40.909092	1
ACGTAGG	150	0.0	40.5	2
CGTTTTT	5530	0.0	40.28029	1
CTCACGA	275	0.0	40.090908	24
ACGGTCT	270	0.0	40.000004	30
ATTACGT	45	1.9292202E-8	40.0	21
TCGATTG	45	1.9292202E-8	40.0	1
GCTACGG	170	0.0	39.705883	2
CGTGCGG	120	0.0	39.375004	2
ACGGGAT	680	0.0	39.04412	5
GATCGAC	75	0.0	39.0	9
CCGGATA	35	6.249875E-6	38.571426	20
ATAGCCG	35	6.249875E-6	38.571426	1
>>END_MODULE