FastQCFastQC Report
Thu 26 May 2016
SRR1547705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547705_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1766962
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88940.5033498173701528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACC207.034318E-445.021
CACGATT207.034318E-445.027
TACGACG253.8916594E-545.01
TTGCGGT253.8916594E-545.011
CGTACTA207.034318E-445.029
GTATCGT207.034318E-445.025
CGTACAT253.8916594E-545.045
ACGTAGC253.8916594E-545.011
GCGCTAA207.034318E-445.023
GTACGTA302.1661672E-644.99999615
CGTTTTT57450.043.315931
TACGGGA6400.041.4843754
TTGCGAG2100.040.7142871
TATTACG501.0822987E-940.51
ACGGGAC4350.040.344835
TCACGAC5800.040.34482625
TAGTACG900.040.01
CGACGGT5950.039.70588328
CTACGCT403.459627E-739.37515
CGAGGGT2000.039.3754
Produced by FastQC (version 0.11.5)