##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547705_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1766962
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.630030526972284	31.0	31.0	33.0	30.0	34.0
2	32.009270714367375	33.0	31.0	34.0	30.0	34.0
3	32.091535075457195	33.0	31.0	34.0	30.0	34.0
4	35.77992395988142	37.0	35.0	37.0	35.0	37.0
5	35.713483934572444	37.0	35.0	37.0	33.0	37.0
6	35.763432377153556	37.0	35.0	37.0	33.0	37.0
7	36.11816722713901	37.0	35.0	37.0	35.0	37.0
8	36.01635745420671	37.0	35.0	37.0	35.0	37.0
9	37.82413543698166	39.0	38.0	39.0	35.0	39.0
10	37.42637136508878	39.0	37.0	39.0	35.0	39.0
11	37.11589779519876	39.0	37.0	39.0	33.0	39.0
12	36.4827494875385	39.0	35.0	39.0	33.0	39.0
13	36.24245286542665	39.0	35.0	39.0	32.0	39.0
14	37.225892237637254	39.0	35.0	41.0	32.0	41.0
15	37.4410089181318	39.0	35.0	41.0	33.0	41.0
16	37.54794613579692	39.0	35.0	41.0	33.0	41.0
17	37.44367790591988	39.0	35.0	41.0	33.0	41.0
18	37.352414483163756	39.0	35.0	41.0	33.0	41.0
19	37.31746466534085	39.0	35.0	41.0	32.0	41.0
20	37.25079373523596	39.0	35.0	41.0	32.0	41.0
21	37.10539558858651	39.0	35.0	41.0	32.0	41.0
22	37.06437433289454	39.0	35.0	41.0	32.0	41.0
23	36.97971093888833	39.0	35.0	41.0	32.0	41.0
24	36.967039472269356	38.0	35.0	41.0	32.0	41.0
25	36.88444856199511	38.0	35.0	41.0	32.0	41.0
26	36.8383972037882	38.0	35.0	41.0	32.0	41.0
27	36.766260960903516	38.0	35.0	41.0	32.0	41.0
28	36.76892825086222	38.0	35.0	41.0	32.0	41.0
29	36.712189622640444	38.0	35.0	41.0	31.0	41.0
30	36.71052348607384	38.0	35.0	41.0	31.0	41.0
31	36.58639744374808	38.0	35.0	41.0	31.0	41.0
32	36.4047483760262	38.0	35.0	41.0	31.0	41.0
33	36.33384305944327	38.0	35.0	41.0	30.0	41.0
34	36.16368093937504	38.0	35.0	41.0	30.0	41.0
35	36.06124636523027	38.0	35.0	41.0	30.0	41.0
36	35.99688561497078	38.0	35.0	41.0	30.0	41.0
37	35.733043494993105	38.0	35.0	41.0	29.0	41.0
38	35.85393517234666	38.0	35.0	41.0	30.0	41.0
39	35.782182072959124	38.0	35.0	40.0	29.0	41.0
40	35.675380115701415	38.0	35.0	40.0	29.0	41.0
41	35.618159869878355	38.0	35.0	40.0	29.0	41.0
42	35.62759980124078	38.0	35.0	40.0	29.0	41.0
43	35.52828640344274	38.0	35.0	40.0	28.0	41.0
44	35.39681441932537	38.0	34.0	40.0	28.0	41.0
45	35.394686473166935	38.0	34.0	40.0	28.0	41.0
46	35.22817638409881	37.0	34.0	40.0	27.0	41.0
47	35.12425847301753	37.0	34.0	40.0	27.0	41.0
48	34.995121570243164	37.0	34.0	40.0	27.0	41.0
49	35.062773845730696	37.0	34.0	40.0	27.0	41.0
50	34.94827336411309	37.0	34.0	40.0	27.0	41.0
51	34.32219481799835	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	3.0
12	12.0
13	12.0
14	17.0
15	34.0
16	127.0
17	263.0
18	625.0
19	1203.0
20	2142.0
21	3415.0
22	5361.0
23	7812.0
24	11272.0
25	16369.0
26	22630.0
27	26255.0
28	28936.0
29	31778.0
30	37704.0
31	45176.0
32	57459.0
33	76038.0
34	141025.0
35	235949.0
36	109793.0
37	147237.0
38	246681.0
39	511513.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.661440370534283	24.287392711331655	30.380845768047077	14.670321150086984
2	29.08076121614387	25.09640841172589	32.32446424993859	13.49836612219165
3	29.885871908960127	24.56770434225524	32.05315111473818	13.49327263404646
4	26.71806184852872	27.31637692265029	31.387828374351006	14.577732854469987
5	24.38705529603919	31.96407166651009	29.291688219667428	14.357184817783292
6	21.8493097191677	41.36500954746056	26.369384287834148	10.416296445537595
7	88.32917742430227	3.9590551466302046	6.178231337176465	1.5335360918910537
8	90.17075636035183	2.6771939634242274	5.758414725387417	1.3936349508365204
9	86.3951799755739	4.895691022217796	6.544056974626505	2.1650720275818043
10	45.9900099719179	32.4394072990817	12.217693419552882	9.352889309447516
11	37.705338315142036	21.682809251132735	24.8626173058617	15.749235127863532
12	35.98447504813346	21.80222325098106	26.089242439848736	16.12405926103674
13	25.238007382162152	30.87321628874871	26.877148461596796	17.01162786749234
14	17.49064213039103	35.6923352058505	28.931012664675304	17.88600999908317
15	16.631993217737563	27.851985498273308	40.02293201551589	15.49308926847323
16	21.55088790817233	24.864258540930706	38.0879724634712	15.496881087425763
17	21.08285294194216	24.271036954954322	28.083343048690352	26.562767054413168
18	22.81746862694274	26.68302996895236	31.798703084729606	18.700798319375288
19	25.598230182652486	28.078306154857884	27.372178914996475	18.951284747493155
20	28.770680976727288	27.30760480417802	26.42145105554053	17.50026316355417
21	25.588665743802075	26.420488952224215	30.009360699324606	17.981484604649108
22	23.535763644039882	22.219096958508448	30.903833811932575	23.341305585519102
23	20.302134397910084	26.31448780449155	30.42770585898282	22.955671938615545
24	21.434020652396597	25.930438798344273	34.506514571337696	18.129025977921426
25	20.344240566576985	26.921065648270876	32.28530098553336	20.44939279961878
26	18.458857632478797	32.80064879720107	27.65911208050881	21.081381489811328
27	18.465535761380266	33.172190460236266	30.825903443311176	17.536370335072288
28	16.0057771474429	29.404254307676116	35.80824035830991	18.781728186571076
29	17.89149964741743	27.40743717182373	34.56752324045452	20.133539940304317
30	19.45163506628892	28.87147544768931	32.932853111725095	18.744036374296673
31	26.529489598531264	26.605552354832756	28.11588477850684	18.74907326812914
32	26.551957540682817	27.152762764564265	28.432812929763063	17.862466764989854
33	24.960921627063854	28.066987292313023	27.360011137760743	19.61207994286238
34	20.428679281161678	28.371860854958964	29.471261973941715	21.728197889937643
35	19.760866391014634	27.987755254498964	30.495336062688388	21.756042291798014
36	26.241481141077173	26.201185990417454	28.960837867481022	18.59649500102436
37	21.059253113536116	30.157637798662336	29.87376072603712	18.90934836176443
38	20.394043561774392	31.285336074007255	25.92042160499207	22.40019875922629
39	20.226015047295867	30.156675695346024	28.65754894559136	20.95976031176675
40	23.09845938961902	27.177098319035725	27.425038003080992	22.299404288264263
41	19.48083773165467	25.907348318752753	29.091683918499662	25.520130031092915
42	21.57714766927642	26.702441818216805	27.629003906139467	24.091406606367315
43	22.22543552153357	26.779579866460058	27.630588546895744	23.364396065110622
44	20.3366003343592	29.725710003950283	28.801751254412945	21.135938407277578
45	19.35327415077404	34.03604604966038	26.215900511725778	20.394779287839807
46	23.13326489194448	30.29533176152062	27.035103188410393	19.536300158124508
47	21.555132481626657	29.413139614773833	27.2987194970803	21.73300840651921
48	22.271559886403896	27.585369691029012	29.38942659774234	20.753643824824756
49	21.527854022893532	25.46183788898686	30.77876038081181	22.231547707307797
50	20.32330067086898	29.762439712908375	29.30266751633595	20.611592099886696
51	19.31773292238316	31.711321465883252	27.45571212057758	21.515233491156007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1182.0
1	2116.0
2	3050.0
3	9308.0
4	15566.0
5	11076.0
6	6586.0
7	6896.0
8	7206.0
9	7511.5
10	7817.0
11	7953.0
12	8089.0
13	7692.0
14	7295.0
15	6883.0
16	6471.0
17	6397.5
18	6324.0
19	5978.0
20	5632.0
21	6301.5
22	6971.0
23	8751.5
24	10532.0
25	11452.5
26	15782.0
27	19191.0
28	23844.0
29	28497.0
30	31828.0
31	35159.0
32	41692.0
33	48225.0
34	52079.5
35	55934.0
36	61638.5
37	67343.0
38	75691.0
39	84039.0
40	99854.0
41	115669.0
42	134239.5
43	152810.0
44	155093.5
45	157377.0
46	160137.5
47	162898.0
48	155019.0
49	147140.0
50	134166.5
51	121193.0
52	111969.5
53	102746.0
54	94343.0
55	85940.0
56	79867.0
57	73794.0
58	68331.0
59	62868.0
60	56807.0
61	50746.0
62	42609.5
63	34473.0
64	28627.5
65	22782.0
66	18362.5
67	13943.0
68	11012.5
69	8082.0
70	6561.5
71	5041.0
72	3874.0
73	2707.0
74	2206.5
75	1239.5
76	773.0
77	623.0
78	473.0
79	312.0
80	151.0
81	114.5
82	78.0
83	52.0
84	26.0
85	25.5
86	25.0
87	19.5
88	14.0
89	13.5
90	13.0
91	10.5
92	8.0
93	4.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1766962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.075541532769133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.89701368352115	14.803328722462483
2	5.9925508408778265	2.166372032230355
3	1.8058799099609124	0.9792677194707557
4	0.9208536401810354	0.6657971287477579
5	0.5982500707372466	0.5406847000296586
6	0.42035552243102875	0.45588922225385553
7	0.3698745192030721	0.46799775636377006
8	0.2889940657891938	0.4178979391117109
9	0.2395177782561348	0.3896472193834816
>10	3.998482142147656	18.55235128959804
>50	2.2116282800352387	29.320480925690447
>100	1.2512536528914817	30.01712097387752
>500	0.003773572212789209	0.41533816893399234
>1k	0.0012578574042630699	0.30228115264690997
>5k	3.1446435106576747E-4	0.505545049199251
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8894	0.5033498173701528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02309047959152489	0.0
2	0.0	0.0	0.0	0.06734723214194759	0.0
3	0.0	0.0	0.0	0.0880607505990508	0.0
4	0.0	0.0	0.0	0.11579196383397039	0.0
5	0.0	0.0	0.0	0.1926470405136047	0.0
6	0.0	0.0	0.0	0.26259761104087126	0.0
7	0.0	0.0	0.0	0.3229837427177268	0.0
8	0.0	0.0	0.0	0.45445233117633543	0.0
9	0.0	0.0	0.0	0.494690887523331	0.0
10	0.0	0.0	0.0	0.5768092352863277	0.0
11	0.0	0.0	0.0	0.714842764020958	0.0
12	0.0	0.0	0.0	0.8308045107931014	0.0
13	0.0	0.0	0.0	0.8726843022091024	0.0
14	0.0	0.0	0.0	0.8914736140335785	0.0
15	0.0	0.0	0.0	0.9233362120973739	0.0
16	0.0	0.0	0.0	0.9897779352357323	0.0
17	0.0	0.0	0.0	1.074160055507702	0.0
18	0.0	0.0	0.0	1.172690753960753	0.0
19	0.0	0.0	0.0	1.2347747150193382	0.0
20	0.0	0.0	0.0	1.301612598346767	0.0
21	0.0	0.0	0.0	1.3910882067639259	0.0
22	0.0	0.0	0.0	1.4884304246497662	0.0
23	0.0	0.0	0.0	1.5899606216772064	0.0
24	0.0	0.0	0.0	1.6728146955056193	0.0
25	0.0	0.0	0.0	1.7431614262219561	0.0
26	0.0	0.0	0.0	1.816111495323612	0.0
27	0.0	0.0	0.0	1.8941550525704571	0.0
28	0.0	0.0	0.0	1.9724249870681996	0.0
29	0.0	0.0	0.0	2.061334652358115	0.0
30	0.0	0.0	0.0	2.166769856963534	0.0
31	0.0	0.0	0.0	2.2723748445071257	0.0
32	0.0	0.0	0.0	2.3590207372880685	0.0
33	0.0	0.0	0.0	2.450024392148784	0.0
34	0.0	0.0	0.0	2.5527430697434355	0.0
35	0.0	0.0	0.0	2.674251059162563	0.0
36	0.0	0.0	0.0	2.775045530124587	0.0
37	0.0	0.0	0.0	2.8856874115006437	0.0
38	0.0	0.0	0.0	3.009855333617814	0.0
39	0.0	0.0	0.0	3.185184514437775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCACC	20	7.034318E-4	45.0	21
CACGATT	20	7.034318E-4	45.0	27
TACGACG	25	3.8916594E-5	45.0	1
TTGCGGT	25	3.8916594E-5	45.0	11
CGTACTA	20	7.034318E-4	45.0	29
GTATCGT	20	7.034318E-4	45.0	25
CGTACAT	25	3.8916594E-5	45.0	45
ACGTAGC	25	3.8916594E-5	45.0	11
GCGCTAA	20	7.034318E-4	45.0	23
GTACGTA	30	2.1661672E-6	44.999996	15
CGTTTTT	5745	0.0	43.31593	1
TACGGGA	640	0.0	41.484375	4
TTGCGAG	210	0.0	40.714287	1
TATTACG	50	1.0822987E-9	40.5	1
ACGGGAC	435	0.0	40.34483	5
TCACGAC	580	0.0	40.344826	25
TAGTACG	90	0.0	40.0	1
CGACGGT	595	0.0	39.705883	28
CTACGCT	40	3.459627E-7	39.375	15
CGAGGGT	200	0.0	39.375	4
>>END_MODULE