FastQCFastQC Report
Thu 26 May 2016
SRR1547704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547704_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2185925
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104730.4791106739709734No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38550.17635554742271578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGC207.034684E-445.00000437
CCGTCGA207.034684E-445.00000417
GTCGTAA302.1664036E-645.00000431
ATGCGTA406.8193913E-945.00000442
CGTCCAC551.8189894E-1245.00000435
GTCGAAC253.891965E-545.09
CGCGACG253.891965E-545.028
AATGCGC253.891965E-545.023
CGTCCCG351.2125929E-745.037
CAATCGT253.891965E-545.018
TGCGTAG1100.042.9545481
CGGTCTA5450.042.93577631
CGTTTTT71750.041.268291
ACGGGTA556.184564E-1140.9090925
GCTACGG556.184564E-1140.9090922
TTTCGCG501.0822987E-940.51
AATATCG501.0822987E-940.525
CGACGGT5950.040.08403428
GTTAGCG900.040.01
AGCGAAT900.040.043