##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547704_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2185925
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.711214245685465	31.0	31.0	33.0	30.0	34.0
2	32.08505506822055	33.0	31.0	34.0	30.0	34.0
3	32.142481100678204	33.0	31.0	34.0	30.0	34.0
4	35.82611434518568	37.0	35.0	37.0	35.0	37.0
5	35.76230977732539	37.0	35.0	37.0	35.0	37.0
6	35.83031348285051	37.0	35.0	37.0	35.0	37.0
7	36.16525361117147	37.0	35.0	37.0	35.0	37.0
8	36.056344568089024	37.0	35.0	37.0	35.0	37.0
9	37.8579104955568	39.0	38.0	39.0	35.0	39.0
10	37.5170104189302	39.0	37.0	39.0	35.0	39.0
11	37.18996534647803	39.0	37.0	39.0	34.0	39.0
12	36.45594244999256	38.0	35.0	39.0	33.0	39.0
13	36.16570971099191	38.0	35.0	39.0	32.0	39.0
14	37.16830678088223	39.0	35.0	41.0	33.0	41.0
15	37.386958381463224	39.0	35.0	41.0	33.0	41.0
16	37.51435387764905	39.0	35.0	41.0	33.0	41.0
17	37.44542562073264	39.0	35.0	41.0	33.0	41.0
18	37.34895662019511	39.0	35.0	41.0	33.0	41.0
19	37.309318938206935	39.0	35.0	41.0	33.0	41.0
20	37.20095291466999	39.0	35.0	41.0	33.0	41.0
21	37.05350915516315	38.0	35.0	41.0	32.0	41.0
22	36.9983155872228	38.0	35.0	41.0	32.0	41.0
23	36.93196564383499	38.0	35.0	41.0	32.0	41.0
24	36.91351075631598	38.0	35.0	41.0	32.0	41.0
25	36.846245868453856	38.0	35.0	41.0	32.0	41.0
26	36.82227752553267	38.0	35.0	41.0	32.0	41.0
27	36.72507107974885	38.0	35.0	41.0	32.0	41.0
28	36.73001772704919	38.0	35.0	41.0	32.0	41.0
29	36.67212507290964	38.0	35.0	40.0	32.0	41.0
30	36.67576106225054	38.0	35.0	41.0	32.0	41.0
31	36.53809851664627	38.0	35.0	41.0	31.0	41.0
32	36.326976451616595	38.0	35.0	41.0	31.0	41.0
33	36.24039251117948	38.0	35.0	41.0	31.0	41.0
34	36.073883596189255	38.0	35.0	41.0	30.0	41.0
35	35.982207532280384	38.0	35.0	41.0	30.0	41.0
36	35.88437160469824	38.0	35.0	41.0	30.0	41.0
37	35.6308317988952	38.0	35.0	40.0	29.0	41.0
38	35.74078204878941	38.0	35.0	40.0	30.0	41.0
39	35.70507268090168	38.0	35.0	40.0	30.0	41.0
40	35.586222308633644	38.0	35.0	40.0	29.0	41.0
41	35.54941729473793	38.0	35.0	40.0	29.0	41.0
42	35.5534654665645	38.0	35.0	40.0	29.0	41.0
43	35.46456763155186	37.0	35.0	40.0	29.0	41.0
44	35.337458512986494	37.0	34.0	40.0	29.0	41.0
45	35.32381669087457	37.0	34.0	40.0	29.0	41.0
46	35.14884865674714	37.0	34.0	40.0	28.0	41.0
47	35.04007136566899	37.0	34.0	40.0	27.0	41.0
48	34.927229891235974	36.0	34.0	40.0	27.0	41.0
49	35.00629253062205	36.0	34.0	40.0	27.0	41.0
50	34.85472420142503	36.0	34.0	40.0	27.0	41.0
51	34.2445902764276	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	6.0
13	16.0
14	32.0
15	55.0
16	134.0
17	310.0
18	675.0
19	1293.0
20	2388.0
21	3896.0
22	6207.0
23	9258.0
24	13509.0
25	20433.0
26	27300.0
27	32532.0
28	34927.0
29	38263.0
30	44419.0
31	54678.0
32	69962.0
33	94494.0
34	180666.0
35	335478.0
36	133711.0
37	176100.0
38	288581.0
39	616437.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.114127886363896	23.282546290472	29.75408579891808	13.84924002424603
2	30.8930086805357	25.294509189473562	30.442032549149673	13.370449580841063
3	29.345288607797613	24.865857703260634	32.9294920914487	12.859361597493052
4	26.535814357766164	27.308210482976314	31.31209899699212	14.843876162265401
5	24.052563560048952	32.00768553358418	29.676727243615403	14.263023662751467
6	22.471402266774934	40.68753502521816	26.768347495911343	10.072715212095567
7	87.74619440282719	3.9865960634513997	6.485583906126697	1.7816256275947253
8	88.65725036311859	3.2606333703123394	6.290563491428114	1.7915527751409588
9	84.76727243615403	5.526356119263013	7.433603623180119	2.2727678214028386
10	48.571794549218296	31.373766254560426	12.076306369157223	7.978132827064058
11	39.94734494550362	20.288893717762505	24.440317028260345	15.32344430847353
12	38.11141736335876	20.351201436462823	27.138717019110903	14.398664181067511
13	24.35842949780985	34.12257968594531	26.144218122762673	15.374772693482164
14	16.444571520065875	39.32815627251621	28.759861385912146	15.46741082150577
15	14.645516200235598	27.601861912005216	43.38136029369718	14.371261594062009
16	18.419479167858	23.591934764458983	42.29568718048423	15.692898887198783
17	19.010396056589315	22.902432608621066	28.54516966501596	29.542001669773665
18	21.640495442432837	25.639077278497663	33.96315061129728	18.757276667772224
19	26.891133044363368	26.49729519539783	28.204764573349955	18.406807186888845
20	29.347667463430817	25.54049201139106	27.209304985303707	17.902535539874425
21	23.54705673799421	26.824845317199813	30.665919462012646	18.962178482793327
22	23.668012397497627	22.11141736335876	30.255109393048706	23.965460846094903
23	19.02860345162803	27.938790214668845	29.385729153561996	23.64687718014113
24	19.549069615837688	25.267518327481504	37.75939247686906	17.424019579811752
25	18.116769788533457	25.733499548246165	35.17156352573853	20.978167137481844
26	17.568123334515136	34.15890298157531	28.383956448643023	19.889017235266536
27	17.603760421789403	33.70678316959639	31.91056417763647	16.778892230977732
28	16.31922412708579	28.974598854032045	37.75120372382401	16.954973295058156
29	16.51456477235038	25.673570685179044	37.39025812870981	20.421606413760767
30	18.5257957157725	29.682308404908675	33.349726088498	18.44216979082082
31	27.588869700470052	26.93061289842973	26.871827715955487	18.608689685144732
32	26.3902924391276	28.211169184670105	28.757391035831514	16.641147340370782
33	25.830026190285576	27.886958610199343	27.468463007651224	18.814552191863857
34	20.601393002962133	29.017098024863614	28.10855816187655	22.2729508102977
35	19.672449878198016	27.303407024486205	31.2188204078365	21.805322689479283
36	28.07401900797145	25.080046204695954	29.060054667932338	17.785880119400254
37	20.888914304013177	31.13688713016229	30.173908070953942	17.800290494870595
38	20.028363278703523	32.05018470441575	25.898281048068895	22.02317096881183
39	19.556572160527008	30.333337145601973	29.909580612326593	20.200510081544426
40	22.496471745370954	27.565630110822646	27.615677573567254	22.322220570239143
41	18.439241968503037	25.642599814723745	29.85861820510768	26.059540011665543
42	21.847318640849984	25.854958427210452	27.464528746411705	24.833194185527866
43	22.469343641707745	25.594565229822614	28.326955407893685	23.609135720575956
44	19.51928817319899	29.72238297288333	29.766940768782096	20.991388085135583
45	18.53499090773929	35.59618925626451	25.218340061987487	20.650479774008716
46	22.972197124786987	31.07201756693391	27.22947951096218	18.726305797316925
47	21.431567871724784	29.36111714720313	27.168315472854744	22.038999508217344
48	22.638471127783433	27.129201596578106	30.544552077495794	19.687775198142663
49	21.99004997884191	25.15200658760022	31.34069101181422	21.517252421743656
50	20.665942335624507	30.804716538765053	28.935073252741972	19.594267872868464
51	19.38913732172879	32.19520340359344	26.588057687249105	21.827601587428664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2102.0
1	3029.5
2	3957.0
3	11278.5
4	18600.0
5	13460.0
6	8320.0
7	8940.0
8	9560.0
9	9857.0
10	10154.0
11	10457.5
12	10761.0
13	10383.5
14	10006.0
15	9406.0
16	8806.0
17	8273.0
18	7740.0
19	7886.5
20	8033.0
21	9041.5
22	10050.0
23	12231.0
24	14412.0
25	15569.5
26	21228.0
27	25729.0
28	30509.0
29	35289.0
30	41883.5
31	48478.0
32	54738.0
33	60998.0
34	67981.0
35	74964.0
36	78409.0
37	81854.0
38	92630.0
39	103406.0
40	128655.0
41	153904.0
42	182349.5
43	210795.0
44	209126.5
45	207458.0
46	198862.0
47	190266.0
48	184981.0
49	179696.0
50	163193.0
51	146690.0
52	131618.0
53	116546.0
54	105598.5
55	94651.0
56	86398.5
57	78146.0
58	74565.5
59	70985.0
60	63620.0
61	56255.0
62	49369.5
63	42484.0
64	34170.5
65	25857.0
66	21367.5
67	16878.0
68	14161.5
69	11445.0
70	8959.5
71	6474.0
72	5036.0
73	3598.0
74	2818.5
75	1536.0
76	1033.0
77	640.0
78	247.0
79	251.0
80	255.0
81	214.0
82	173.0
83	108.5
84	44.0
85	31.0
86	18.0
87	13.5
88	9.0
89	9.0
90	9.0
91	10.0
92	11.0
93	10.0
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2185925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.3939909011967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.23782723055302	13.64600182253069
2	6.577348447109492	2.156579811918265
3	1.947165575417457	0.9576544417955166
4	0.8653343768850195	0.5674513560458289
5	0.5293073933700444	0.43387302954223245
6	0.35666009359821926	0.35082493975615014
7	0.2945066499260893	0.3379697537461161
8	0.21229364221497163	0.2784272031083322
9	0.18677561690383007	0.27557979876781113
>10	2.3522221978724023	9.592777939085435
>50	1.4108007406696625	17.148503870605726
>100	2.018830293433561	52.35969137169123
>500	0.008966352448242633	0.9094353626177061
>1k	0.0016811910840454935	0.5041445148363131
>5k	0.0	0.0
>10k+	2.8019851400758227E-4	0.48108478395268395
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10473	0.4791106739709734	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3855	0.17635554742271578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.574722371536077E-5	0.0	0.0	0.019900042316181938	0.0
2	4.574722371536077E-5	0.0	0.0	0.07287532737856971	0.0
3	4.574722371536077E-5	0.0	0.0	0.10352596726786144	0.0
4	4.574722371536077E-5	0.0	0.0	0.15064560769468302	0.0
5	4.574722371536077E-5	0.0	0.0	0.25453755275226736	0.0
6	4.574722371536077E-5	0.0	0.0	0.3472214279995883	0.0
7	4.574722371536077E-5	0.0	0.0	0.40998661893706323	0.0
8	4.574722371536077E-5	0.0	0.0	0.545764378924254	0.0
9	9.149444743072154E-5	0.0	0.0	0.5972757528277503	0.0
10	9.149444743072154E-5	0.0	0.0	0.6895479030616329	0.0
11	9.149444743072154E-5	0.0	0.0	0.8519048000274483	0.0
12	9.149444743072154E-5	0.0	0.0	0.9838855404462642	0.0
13	9.149444743072154E-5	0.0	0.0	1.0315998947813854	0.0
14	9.149444743072154E-5	0.0	0.0	1.0535585621647585	0.0
15	9.149444743072154E-5	0.0	0.0	1.085947596555234	0.0
16	9.149444743072154E-5	0.0	0.0	1.1566270571954664	0.0
17	9.149444743072154E-5	0.0	0.0	1.2428148266752062	0.0
18	9.149444743072154E-5	0.0	0.0	1.3500920662877272	0.0
19	9.149444743072154E-5	0.0	0.0	1.4197193407825062	0.0
20	9.149444743072154E-5	0.0	0.0	1.4874709791049556	0.0
21	9.149444743072154E-5	0.0	0.0	1.581481523840022	0.0
22	9.149444743072154E-5	0.0	0.0	1.6754920685750883	0.0
23	9.149444743072154E-5	0.0	0.0	1.7824490776216018	0.0
24	9.149444743072154E-5	0.0	0.0	1.860082116266569	0.0
25	9.149444743072154E-5	0.0	0.0	1.9266900739961343	0.0
26	9.149444743072154E-5	0.0	0.0	1.9982387318869586	0.0
27	9.149444743072154E-5	0.0	0.0	2.0697416425540673	0.0
28	9.149444743072154E-5	0.0	0.0	2.1451788144606976	0.0
29	9.149444743072154E-5	0.0	0.0	2.2329677367704748	0.0
30	9.149444743072154E-5	0.0	0.0	2.33507554010316	0.0
31	9.149444743072154E-5	0.0	0.0	2.4359481683955306	0.0
32	9.149444743072154E-5	0.0	0.0	2.5240115740476	0.0
33	9.149444743072154E-5	0.0	0.0	2.607957729565287	0.0
34	9.149444743072154E-5	0.0	0.0	2.7038896576963984	0.0
35	9.149444743072154E-5	0.0	0.0	2.8282763589784645	0.0
36	9.149444743072154E-5	0.0	0.0	2.9298351956265654	0.0
37	9.149444743072154E-5	0.0	0.0	3.0309823072612283	0.0
38	1.372416711460823E-4	0.0	0.0	3.1373903496231574	0.0
39	1.372416711460823E-4	0.0	0.0	3.2612738314443543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGC	20	7.034684E-4	45.000004	37
CCGTCGA	20	7.034684E-4	45.000004	17
GTCGTAA	30	2.1664036E-6	45.000004	31
ATGCGTA	40	6.8193913E-9	45.000004	42
CGTCCAC	55	1.8189894E-12	45.000004	35
GTCGAAC	25	3.891965E-5	45.0	9
CGCGACG	25	3.891965E-5	45.0	28
AATGCGC	25	3.891965E-5	45.0	23
CGTCCCG	35	1.2125929E-7	45.0	37
CAATCGT	25	3.891965E-5	45.0	18
TGCGTAG	110	0.0	42.954548	1
CGGTCTA	545	0.0	42.935776	31
CGTTTTT	7175	0.0	41.26829	1
ACGGGTA	55	6.184564E-11	40.909092	5
GCTACGG	55	6.184564E-11	40.909092	2
TTTCGCG	50	1.0822987E-9	40.5	1
AATATCG	50	1.0822987E-9	40.5	25
CGACGGT	595	0.0	40.084034	28
GTTAGCG	90	0.0	40.0	1
AGCGAAT	90	0.0	40.0	43
>>END_MODULE