FastQCFastQC Report
Thu 26 May 2016
SRR1547703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547703_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1615797
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90800.5619517798337291No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30300.18752355648636554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG207.0341374E-445.020
GGCGTAG800.045.01
CGTTCGA207.0341374E-445.025
TAACCGT253.8915106E-545.027
TATTCGT253.8915106E-545.033
GCGATCG207.0341374E-445.09
TCGTAAG1000.045.01
ATAACCG253.8915106E-545.026
TCGACTA207.0341374E-445.037
TCGCGAC207.0341374E-445.019
ACGACGT207.0341374E-445.021
CGACGGT4150.044.45783228
GCGATAA1250.043.29
TCACGAC4450.042.97752825
CTCACGA4500.042.524
TCTCACG4500.042.523
CGTTTTT59150.042.3753171
CGCATCG850.042.3529421
ACGGGTC750.042.0000045
CGGTCTA4350.041.89655331