FastQCFastQC Report
Thu 26 May 2016
SRR1547702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547702_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1398291
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60280.4310976756626482No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21920.1567627911500539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTC253.8912403E-545.00000416
CGACTAT253.8912403E-545.00000417
GTACGTT253.8912403E-545.00000440
GTCGACG207.033812E-445.012
CACGCGT207.033812E-445.043
CACGATA406.8175723E-945.016
CACGACG1900.045.026
CGTTCGA207.033812E-445.043
CTAACCG207.033812E-445.01
TACGACG207.033812E-445.01
ACGTCCG207.033812E-445.024
CGCACCG207.033812E-445.026
CGACTAA406.8175723E-945.06
CGGTACG207.033812E-445.031
CCACGCG207.033812E-445.042
TCGACTA207.033812E-445.045
ATCGACT207.033812E-445.044
AATCGAC207.033812E-445.043
CGGACTA207.033812E-445.020
CGACGGT2050.041.7073228