##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547702_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1398291
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.661511087463197	31.0	31.0	33.0	30.0	34.0
2	32.045316747372325	33.0	31.0	34.0	30.0	34.0
3	32.10505753094313	33.0	31.0	34.0	30.0	34.0
4	35.79812070591887	37.0	35.0	37.0	35.0	37.0
5	35.72228813601747	37.0	35.0	37.0	33.0	37.0
6	35.786553013643086	37.0	35.0	37.0	35.0	37.0
7	36.12919914381198	37.0	35.0	37.0	35.0	37.0
8	36.02728974154879	37.0	35.0	37.0	35.0	37.0
9	37.83272294536688	39.0	38.0	39.0	35.0	39.0
10	37.46991792123385	39.0	37.0	39.0	35.0	39.0
11	37.1144354072221	39.0	37.0	39.0	33.0	39.0
12	36.40549785416626	38.0	35.0	39.0	33.0	39.0
13	36.13964403689933	38.0	35.0	39.0	32.0	39.0
14	37.111634130520756	39.0	35.0	41.0	32.0	41.0
15	37.359476675455966	39.0	35.0	41.0	33.0	41.0
16	37.49363472982376	39.0	35.0	41.0	33.0	41.0
17	37.41550578527646	39.0	35.0	41.0	33.0	41.0
18	37.33211899382889	39.0	35.0	41.0	33.0	41.0
19	37.302486392317476	39.0	35.0	41.0	33.0	41.0
20	37.19082723124156	39.0	35.0	41.0	33.0	41.0
21	37.04338724914914	39.0	35.0	41.0	32.0	41.0
22	36.99584921879637	39.0	35.0	41.0	32.0	41.0
23	36.92681137188182	38.0	35.0	41.0	32.0	41.0
24	36.90850616931669	38.0	35.0	41.0	32.0	41.0
25	36.84900925486898	38.0	35.0	41.0	32.0	41.0
26	36.79935864566102	38.0	35.0	41.0	32.0	41.0
27	36.71825249536756	38.0	35.0	41.0	32.0	41.0
28	36.7354327532681	38.0	35.0	41.0	32.0	41.0
29	36.69054367080958	38.0	35.0	41.0	32.0	41.0
30	36.709699912250024	38.0	35.0	41.0	32.0	41.0
31	36.56146181302748	38.0	35.0	41.0	31.0	41.0
32	36.3789032468921	38.0	35.0	41.0	31.0	41.0
33	36.311056139244265	38.0	35.0	41.0	31.0	41.0
34	36.15017617935037	38.0	35.0	41.0	30.0	41.0
35	36.05741222678255	38.0	35.0	41.0	30.0	41.0
36	35.94737790631564	38.0	35.0	41.0	30.0	41.0
37	35.69043925763664	38.0	35.0	40.0	29.0	41.0
38	35.8134851758325	38.0	35.0	40.0	30.0	41.0
39	35.76738318418698	38.0	35.0	40.0	29.0	41.0
40	35.65787450537835	38.0	35.0	40.0	29.0	41.0
41	35.61114031342546	38.0	35.0	40.0	29.0	41.0
42	35.62518817613787	38.0	35.0	40.0	29.0	41.0
43	35.53343116704606	38.0	35.0	40.0	29.0	41.0
44	35.417983095078206	38.0	34.0	40.0	29.0	41.0
45	35.40495290322258	37.0	34.0	40.0	28.0	41.0
46	35.24260972859012	37.0	34.0	40.0	28.0	41.0
47	35.12891594095936	37.0	34.0	40.0	27.0	41.0
48	35.020306931818915	37.0	34.0	40.0	27.0	41.0
49	35.10062569236303	37.0	34.0	40.0	27.0	41.0
50	34.95855226129611	37.0	34.0	40.0	27.0	41.0
51	34.346366385823835	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	5.0
13	4.0
14	22.0
15	29.0
16	75.0
17	257.0
18	476.0
19	933.0
20	1665.0
21	2646.0
22	4106.0
23	5956.0
24	8841.0
25	13091.0
26	17813.0
27	20679.0
28	22525.0
29	24370.0
30	28906.0
31	35817.0
32	45350.0
33	60744.0
34	113758.0
35	203583.0
36	83540.0
37	111438.0
38	186531.0
39	405024.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.40891917347676	23.773020065208172	30.008703481607192	13.809357279707871
2	30.81182672276372	25.268703009602433	30.243490088972898	13.675980178660952
3	29.739160160510224	24.341070635511493	32.35857199967675	13.561197204301537
4	26.707459319984178	27.182324709234347	31.05004609197942	15.060169878802052
5	24.20168620122707	32.17248770105793	29.399745832591357	14.226080265123638
6	22.779163993760957	40.40160453010139	26.05387576691833	10.765355709219325
7	88.3615070110585	3.82502640723569	6.168172433348995	1.6452941483568155
8	89.55789603165579	2.9807100238791495	5.8911199457051495	1.5702739987599148
9	85.61794361831693	5.319922677039329	6.729142932336687	2.332990772307052
10	48.40930821981976	32.48250900563617	11.513054149672708	7.595128624871361
11	39.39666349851354	21.166838662338527	25.37662046026185	14.059877378886082
12	36.68735620840011	21.80468872359187	26.747365176490444	14.760589891517572
13	23.307022644070514	34.84567947587448	25.85499012723389	15.992307752821123
14	15.472458880161568	39.292965484294754	29.56401779028829	15.670557845255386
15	14.737847844261315	27.842630754256447	43.195086001411724	14.224435400070515
16	19.002482315912783	24.27026992235522	41.35154985621734	15.37569790551466
17	19.40125481748792	23.50483554567683	28.69910483583174	28.39480480100351
18	21.80454569184812	26.115736996090227	33.73274947775535	18.34696783430631
19	26.572580385627887	26.664907376218537	27.65332824140326	19.109183996750318
20	28.723777811628626	27.09285835351869	27.222159049868733	16.961204784983956
21	23.93199984838635	26.776329104599828	30.785937977144958	18.505733069868864
22	23.457635070239313	22.794825969701584	30.316579310029173	23.43095965002993
23	18.716347312540808	28.019131926044004	29.824764659144627	23.439756102270557
24	19.302848977787885	26.32384818324655	37.712035620625464	16.661267218340104
25	18.397171976362575	27.946042704987732	33.716229311352215	19.94055600729748
26	17.407535341355985	35.274417127765254	27.608058694506365	19.7099888363724
27	17.33394550919658	34.128732860327354	31.419711633701425	17.117609996774636
28	15.451361697958435	30.185133137522875	37.42647274422849	16.937032420290198
29	16.309623676330606	25.98143018870893	37.744432310584855	19.96451382437561
30	19.026297101247167	30.38830972951982	32.379955245367384	18.205437923865635
31	26.256480231940277	28.47690502191604	27.23589009726874	18.030724648874948
32	26.707387804112305	28.292966199453474	28.403815800859768	16.595830195574454
33	26.089776734599596	28.797367643788025	26.94946903040926	18.163386591203118
34	19.00348353811903	29.8884137851134	29.115756305375633	21.992346371391935
35	19.596064052475487	28.416617141925393	30.94634807776064	21.04097072783848
36	27.101583289887444	26.06367344136521	29.082358393209994	17.752384875537352
37	20.158965480003804	31.437805149285808	30.495941116691732	17.907288254018656
38	19.519613585441085	31.988763426210998	26.644024741631036	21.847598246716885
39	19.480851982884822	31.070428115463805	29.40103311828511	20.047686783366267
40	23.010088744045408	27.376275753759412	27.711113065878273	21.902522436316904
41	18.654843662728286	26.275145874499657	29.677441963082074	25.392568499689975
42	21.767285922601232	25.467374101671254	27.921298213318973	24.84404176240854
43	22.10312445692635	27.083918869534312	27.436706665493805	23.376250008045535
44	19.374722429022285	30.246851334951025	29.56437536964766	20.81405086637903
45	18.391450706612574	35.906474403396714	25.098852813899253	20.603222076091456
46	22.479011879501478	31.88249084060471	26.713609684965434	18.92488759492838
47	21.215684002829168	30.027726703525946	27.187187788521843	21.56940150512304
48	22.377030246207692	26.859072968359232	30.963940982241894	19.799955803191178
49	21.19587410631979	25.583086782365044	31.72601411294216	21.49502499837301
50	19.75397109757554	31.95028788714223	28.46038485551291	19.83535615976932
51	18.682234241656424	33.46957106925525	26.05716549702458	21.79102919206374
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	940.0
1	1545.0
2	2150.0
3	6324.5
4	10499.0
5	7668.0
6	4837.0
7	5105.0
8	5373.0
9	5753.0
10	6133.0
11	6123.0
12	6113.0
13	5966.5
14	5820.0
15	5719.5
16	5619.0
17	5420.5
18	5222.0
19	5499.0
20	5776.0
21	5874.0
22	5972.0
23	8023.0
24	10074.0
25	11157.5
26	13651.5
27	15062.0
28	19240.5
29	23419.0
30	27544.0
31	31669.0
32	37200.5
33	42732.0
34	46095.0
35	49458.0
36	52792.5
37	56127.0
38	61540.0
39	66953.0
40	83764.0
41	100575.0
42	118435.0
43	136295.0
44	140196.0
45	144097.0
46	136042.5
47	127988.0
48	119586.0
49	111184.0
50	104464.0
51	97744.0
52	88826.5
53	79909.0
54	69978.5
55	60048.0
56	54628.0
57	49208.0
58	44833.0
59	40458.0
60	34754.5
61	29051.0
62	23859.0
63	18667.0
64	15518.0
65	12369.0
66	9909.5
67	7450.0
68	5930.0
69	4410.0
70	3582.0
71	2754.0
72	2305.0
73	1856.0
74	1459.0
75	830.0
76	598.0
77	376.5
78	155.0
79	135.5
80	116.0
81	90.0
82	64.0
83	46.0
84	28.0
85	18.0
86	8.0
87	4.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1398291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.327236091460854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7908371816918	14.989999831471005
2	6.002302632193242	2.2001123486520493
3	1.7703967962313734	0.9733944018029487
4	0.8988246147769308	0.6589188367933265
5	0.5869119162599626	0.537823662709402
6	0.4308126178730509	0.4737362735363827
7	0.34960603080182007	0.44851185858524434
8	0.2818398624411188	0.4132276559154588
9	0.2537298159491654	0.4185149616308937
>10	4.067155197981742	19.273934706378054
>50	2.466574284806793	33.01655276139922
>100	1.0994389826129536	25.922585671025345
>500	7.850331900128195E-4	0.08136124810650137
>1k	3.9251659500640974E-4	0.1576868751984536
>5k	3.9251659500640974E-4	0.4336389067957474
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6028	0.4310976756626482	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2192	0.1567627911500539	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019809896509381808	0.0
2	0.0	0.0	0.0	0.07859594319065202	0.0
3	0.0	0.0	0.0	0.11156476012503835	0.0
4	0.0	0.0	0.0	0.15990948951255496	0.0
5	0.0	0.0	0.0	0.2839895272157226	0.0
6	0.0	0.0	0.0	0.3876875414345083	0.0
7	0.0	0.0	0.0	0.4575585482564073	0.0
8	0.0	0.0	0.0	0.604952760190833	0.0
9	0.0	0.0	0.0	0.6505083705752236	0.0
10	0.0	0.0	0.0	0.7463396388877566	0.0
11	0.0	0.0	0.0	0.9014575649846849	0.0
12	0.0	0.0	0.0	1.015310833009724	0.0
13	0.0	0.0	0.0	1.061438570369115	0.0
14	0.0	0.0	0.0	1.0818205938534968	0.0
15	0.0	0.0	0.0	1.1152185060191333	0.0
16	0.0	0.0	0.0	1.1918120047972847	0.0
17	0.0	0.0	0.0	1.283137773181691	0.0
18	0.0	0.0	0.0	1.4048577871129828	0.0
19	0.0	0.0	0.0	1.4766597224755076	0.0
20	0.0	0.0	0.0	1.5513938085849084	0.0
21	0.0	0.0	0.0	1.6503717752599423	0.0
22	0.0	0.0	0.0	1.762866241719356	0.0
23	0.0	0.0	0.0	1.8846577715225228	0.0
24	0.0	0.0	0.0	1.9742671589819287	0.0
25	0.0	0.0	0.0	2.048357602244454	0.0
26	0.0	0.0	0.0	2.1267389978194813	0.0
27	0.0	0.0	0.0	2.2024027902632572	0.0
28	0.0	0.0	0.0	2.2862909079726608	0.0
29	0.0	0.0	0.0	2.3864131285976953	0.0
30	0.0	0.0	0.0	2.500838523597735	0.0
31	0.0	0.0	0.0	2.6136190535446486	0.0
32	0.0	0.0	0.0	2.7070187822134306	0.0
33	0.0	0.0	0.0	2.804566431450964	0.0
34	0.0	0.0	0.0	2.9142002630353767	0.0
35	0.0	0.0	0.0	3.046933721235422	0.0
36	0.0	0.0	0.0	3.1645773304698377	0.0
37	0.0	0.0	0.0	3.2851530904511295	0.0
38	0.0	0.0	0.0	3.4106634455917972	0.0
39	0.0	0.0	0.0	3.587307649123108	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGTC	25	3.8912403E-5	45.000004	16
CGACTAT	25	3.8912403E-5	45.000004	17
GTACGTT	25	3.8912403E-5	45.000004	40
GTCGACG	20	7.033812E-4	45.0	12
CACGCGT	20	7.033812E-4	45.0	43
CACGATA	40	6.8175723E-9	45.0	16
CACGACG	190	0.0	45.0	26
CGTTCGA	20	7.033812E-4	45.0	43
CTAACCG	20	7.033812E-4	45.0	1
TACGACG	20	7.033812E-4	45.0	1
ACGTCCG	20	7.033812E-4	45.0	24
CGCACCG	20	7.033812E-4	45.0	26
CGACTAA	40	6.8175723E-9	45.0	6
CGGTACG	20	7.033812E-4	45.0	31
CCACGCG	20	7.033812E-4	45.0	42
TCGACTA	20	7.033812E-4	45.0	45
ATCGACT	20	7.033812E-4	45.0	44
AATCGAC	20	7.033812E-4	45.0	43
CGGACTA	20	7.033812E-4	45.0	20
CGACGGT	205	0.0	41.70732	28
>>END_MODULE