FastQCFastQC Report
Thu 26 May 2016
SRR1547701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547701_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1132045
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64010.5654368863428574No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28860.25493686205053684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCA253.8907674E-545.00000436
CTATGCG351.2119017E-745.0000041
CCCCGTT351.2119017E-745.00000430
CTAAACG253.8907674E-545.0000041
ACTACGT253.8907674E-545.00000418
ACTACGC253.8907674E-545.00000439
CTCGATA253.8907674E-545.00000424
CGTCGCA253.8907674E-545.00000431
AATCGTT253.8907674E-545.00000422
TGATCGC207.0332416E-445.019
ACGCCCG207.0332416E-445.026
GCGCGAA207.0332416E-445.013
CGTACGA207.0332416E-445.041
GCGGCAC207.0332416E-445.05
CATCGTA207.0332416E-445.028
CGATCTT551.8189894E-1245.010
TGTAACG406.8157533E-945.01
CCGTTGT207.0332416E-445.038
GCGTAGC207.0332416E-445.016
TAGTCCG207.0332416E-445.01