FastQCFastQC Report
Thu 26 May 2016
SRR1547700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547700_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1571185
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.45271562546740196No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23040.14664091115941152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC351.2123019E-745.00000414
CGAACCG207.0340786E-445.028
CGATTCG1000.045.010
TACGCGG1050.045.02
ACGTGCG207.0340786E-445.01
GTCGTAG453.8562575E-1045.01
CCGAACG453.8562575E-1045.036
ACGCGTA207.0340786E-445.030
CGGACTA207.0340786E-445.011
TCGTACT302.1660126E-644.99999611
TATAGCG700.041.7857171
CGTTTTT48600.041.759261
ATGACGG1800.041.252
TACGGGA7100.040.563384
CGGGATC4300.040.29076
CGTTAGG1300.039.807692
GGCGATA3450.039.7826088
GGTACGA403.4592995E-739.3759
TTCGTCA403.4592995E-739.37528
TAGGGAC15750.039.2857135
Produced by FastQC (version 0.11.5)