FastQCFastQC Report
Thu 26 May 2016
SRR1547699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547699_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1301509
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73080.5615020718258575No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA22140.17011023358271055No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16210.1245477365120026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC253.8910905E-545.0000048
CGCGATT302.1657233E-645.00000423
GTTCGCG302.1657233E-645.0000041
CGGACCG253.8910905E-545.0000046
GCGGTAT207.033631E-445.036
GTTCGAC207.033631E-445.038
TCGACTC207.033631E-445.024
CGGTCTA1800.042.531
CGTTTTT45350.042.320841
TACGTAG800.042.18751
GTATGCG650.041.538461
TAGTACG650.041.538461
TTAGGCG556.184564E-1140.9090921
TTTAGCG1600.040.781251
TACGCGG501.0822987E-940.5000042
ACGGTCT1900.040.2631630
CACGACG1850.040.13513626
GGCGTAG900.040.01
TACGGGA2650.039.9056634
TAAACGG1200.039.3750042