##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547699_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1301509
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.578326388830195	31.0	31.0	33.0	30.0	34.0
2	31.968974475013233	31.0	31.0	34.0	30.0	34.0
3	32.04334199763505	33.0	31.0	34.0	30.0	34.0
4	35.75295829686925	37.0	35.0	37.0	35.0	37.0
5	35.678062925419646	37.0	35.0	37.0	33.0	37.0
6	35.72892158256301	37.0	35.0	37.0	33.0	37.0
7	36.075407853499286	37.0	35.0	37.0	35.0	37.0
8	35.9757112705329	37.0	35.0	37.0	35.0	37.0
9	37.80603438009265	39.0	38.0	39.0	35.0	39.0
10	37.36972698613686	39.0	37.0	39.0	34.0	39.0
11	37.107444512485124	39.0	37.0	39.0	33.0	39.0
12	36.45952890068374	38.0	35.0	39.0	33.0	39.0
13	36.19550844442874	38.0	35.0	39.0	32.0	39.0
14	37.180999132545374	39.0	35.0	41.0	32.0	41.0
15	37.40230071401734	39.0	35.0	41.0	33.0	41.0
16	37.51130495447976	39.0	35.0	41.0	33.0	41.0
17	37.43198241425914	39.0	35.0	41.0	33.0	41.0
18	37.322673911590314	39.0	35.0	41.0	32.0	41.0
19	37.28322892888178	39.0	35.0	41.0	32.0	41.0
20	37.179421732773264	39.0	35.0	41.0	32.0	41.0
21	37.03467436644695	39.0	35.0	41.0	32.0	41.0
22	36.9954237734814	39.0	35.0	41.0	32.0	41.0
23	36.91095720429133	38.0	35.0	41.0	32.0	41.0
24	36.88664081462364	38.0	35.0	41.0	32.0	41.0
25	36.81975998629284	38.0	35.0	41.0	32.0	41.0
26	36.778491735362564	38.0	35.0	41.0	32.0	41.0
27	36.68500256241025	38.0	35.0	41.0	31.0	41.0
28	36.69196063953457	38.0	35.0	41.0	31.0	41.0
29	36.64842886218997	38.0	35.0	40.0	31.0	41.0
30	36.64861095851047	38.0	35.0	41.0	31.0	41.0
31	36.51771059593134	38.0	35.0	41.0	31.0	41.0
32	36.324432639344025	38.0	35.0	41.0	31.0	41.0
33	36.250891081045154	38.0	35.0	41.0	30.0	41.0
34	36.074691761639755	38.0	35.0	41.0	30.0	41.0
35	35.96445817892923	38.0	35.0	41.0	30.0	41.0
36	35.872392737968006	38.0	35.0	41.0	30.0	41.0
37	35.60431622063312	38.0	35.0	40.0	28.0	41.0
38	35.715504080263756	38.0	35.0	40.0	29.0	41.0
39	35.64754296743242	38.0	35.0	40.0	29.0	41.0
40	35.51828531343233	38.0	35.0	40.0	28.0	41.0
41	35.458361793886944	38.0	35.0	40.0	28.0	41.0
42	35.46848465896125	38.0	35.0	40.0	28.0	41.0
43	35.375172972296006	38.0	34.0	40.0	28.0	41.0
44	35.24533061238916	37.0	34.0	40.0	27.0	41.0
45	35.22401765950139	37.0	34.0	40.0	27.0	41.0
46	35.04771154098819	37.0	34.0	40.0	27.0	41.0
47	34.922763499906644	37.0	34.0	40.0	26.0	41.0
48	34.796076707882925	37.0	34.0	40.0	26.0	41.0
49	34.86262254045112	36.0	34.0	40.0	27.0	41.0
50	34.72312139216863	36.0	34.0	40.0	26.0	41.0
51	34.111184017936104	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	3.0
13	13.0
14	13.0
15	33.0
16	96.0
17	196.0
18	474.0
19	898.0
20	1647.0
21	2540.0
22	3981.0
23	5773.0
24	8676.0
25	12292.0
26	17019.0
27	20623.0
28	22175.0
29	24428.0
30	28363.0
31	34292.0
32	43609.0
33	57893.0
34	104043.0
35	175374.0
36	84263.0
37	112702.0
38	183333.0
39	356680.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.015145496496764	22.529233374490687	30.34877207917886	15.106849049833693
2	30.551152546774553	24.8042848724058	31.29152391570093	13.353038665118719
3	28.782590055082213	23.99699118484774	34.09911110872073	13.121307651349317
4	25.734743286446733	26.358096640130803	32.74437595129961	15.162784122122858
5	23.57002525529981	30.8537244075915	31.782185140479243	13.794065196629452
6	21.114029945240485	39.75915648681646	28.021934539061967	11.104879028881092
7	87.48652525645232	3.577155440338868	6.709903657984693	2.2264156452241206
8	89.38378451474404	2.4990222887432973	6.484626691017888	1.6325665054947758
9	86.21338769074973	3.8125744808526103	7.426917524196913	2.5471203042007393
10	45.69357568791303	25.75410542685452	15.09924249467349	13.45307639055896
11	37.3525653683532	25.436397289607676	22.35512777860161	14.855909563437516
12	35.62695302145433	21.65056100265154	26.04223251625613	16.680253459638006
13	23.543671230855875	31.87569198522638	26.148416952936937	18.43221983098081
14	16.837839769068058	35.35788073689848	29.390115627321823	18.41416386671164
15	15.897008779808669	26.304159248994818	41.5917216093012	16.207110361895307
16	20.759595208331252	22.939372682017567	39.044601305100464	17.256430804550718
17	21.433889431421527	22.453090988998156	27.436537127288403	28.676482452291918
18	23.009522024050543	26.05314292870814	31.782261974369753	19.155073072871566
19	27.248601431108042	25.944576641421612	27.30038747330983	19.50643445416052
20	28.605257435791838	26.239388279297337	26.98037431934777	18.17497996556305
21	24.58623029114666	26.343959204277496	30.266482982445766	18.803327522130083
22	24.940127190822345	20.96919806163461	30.020384031151533	24.07029071639151
23	20.6842979956343	26.53750377446487	28.940022696731255	23.838175533169576
24	20.924327069578467	24.55134770485644	35.38723128307219	19.137093942492907
25	19.79287119797097	26.15417949472497	32.87061403340277	21.182335273901295
26	19.086076239196196	32.06247517304913	27.719285844354513	21.13216274340016
27	19.170977688206538	31.51726188601078	30.316348177384867	18.995412248397823
28	16.869956335300024	28.717665417603722	35.58315770386528	18.829220543230974
29	17.455814750416632	25.67650319744235	35.85653268628953	21.011149365851484
30	19.704281722216287	28.657811816898693	32.41844658776851	19.219459873116513
31	26.918369369708543	25.68825878268994	27.535422344371035	19.857949503230483
32	26.278112560112916	27.22270841000715	28.192966779330764	18.30621225054917
33	25.42126101317778	26.660899002619264	27.240918042057334	20.67692194214562
34	20.67868912162728	27.35063683770147	29.357230722184784	22.613443318486464
35	20.31964435128762	26.711302034791924	30.927331274697295	22.041722339223163
36	27.714983146486116	24.35572861962537	28.831610077225744	19.097678156662766
37	21.10918941013854	29.317200265230593	29.927799193090483	19.645811131540388
38	20.436278197077392	30.664559369163026	26.698470775077237	22.200691658682345
39	20.77288747138898	29.743167354201933	28.30890912010597	21.17503605430312
40	23.494497540931334	26.908841967285667	27.0900162811014	22.5066442106816
41	19.929328187511572	25.11377178336838	29.142479998217453	25.81442003090259
42	22.446252772743026	25.542274390726455	26.574691377470305	25.436781459060214
43	22.590469985224843	25.48818333180946	27.376145689349823	24.54520099361587
44	20.632819288994543	28.757772708448424	28.6243122406376	21.985095761919435
45	19.511812826496016	33.09012845858154	25.42710038885632	21.97095832606613
46	23.19054267008526	29.4721742223834	26.8615891246238	20.475693982907533
47	21.759050456047557	27.619555454476302	27.975142699743145	22.646251389732992
48	23.269374241745545	25.766629351007175	29.524267600147212	21.439728807100067
49	22.217595114593905	24.44185941088383	30.77619901206984	22.56434646245243
50	20.902659912455466	29.007867022049023	29.029380511391008	21.060092554104504
51	19.755453093294015	30.708815690095115	27.25344196620999	22.28228925040088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1163.0
1	1730.0
2	2297.0
3	7530.5
4	12764.0
5	9055.5
6	5347.0
7	5468.0
8	5589.0
9	5795.0
10	6001.0
11	6030.5
12	6060.0
13	5696.0
14	5332.0
15	5090.5
16	4849.0
17	4383.0
18	3917.0
19	3798.0
20	3679.0
21	3997.5
22	4316.0
23	4408.0
24	4500.0
25	6140.0
26	9959.5
27	12139.0
28	13050.5
29	13962.0
30	18868.0
31	23774.0
32	26630.0
33	29486.0
34	31712.5
35	33939.0
36	35424.0
37	36909.0
38	45911.0
39	54913.0
40	69870.5
41	84828.0
42	97816.0
43	110804.0
44	118026.5
45	125249.0
46	121954.0
47	118659.0
48	113848.5
49	109038.0
50	103561.5
51	98085.0
52	90107.5
53	82130.0
54	74251.5
55	66373.0
56	63505.0
57	60637.0
58	55017.0
59	49397.0
60	43220.5
61	37044.0
62	33142.5
63	29241.0
64	24513.0
65	19785.0
66	16352.5
67	12920.0
68	10562.0
69	8204.0
70	6471.0
71	4738.0
72	3595.5
73	2453.0
74	2071.5
75	1250.5
76	811.0
77	586.0
78	361.0
79	278.0
80	195.0
81	129.5
82	64.0
83	62.5
84	61.0
85	37.0
86	13.0
87	10.5
88	8.0
89	5.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1301509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.983107584684166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21607069746648	16.22949478352788
2	5.804738386927174	2.3199342337382345
3	1.8099882264444953	1.085075683681561
4	0.9648907890997708	0.7712606578420607
5	0.631206534221764	0.6306734040754569
6	0.48298599610896964	0.5790936673284832
7	0.3947267285634816	0.5521506678404133
8	0.31305134447576843	0.5004590956951444
9	0.29218926308446286	0.5254964531375766
>10	5.049179979653199	26.433484039252203
>50	2.470499329913201	35.08791180810998
>100	0.568149006224391	14.266721085948205
>500	0.0011618589084343373	0.1558671628428356
>1k	7.745726056228915E-4	0.2967977008452207
>5k	3.8728630281144576E-4	0.5655795561347778
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7308	0.5615020718258575	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	2214	0.17011023358271055	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1621	0.1245477365120026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029811549516753244	0.0
2	0.0	0.0	0.0	0.10679910780486343	0.0
3	0.0	0.0	0.0	0.14921141536478041	0.0
4	0.0	0.0	0.0	0.2224341130180429	0.0
5	0.0	0.0	0.0	0.41882153715418025	0.0
6	0.0	0.0	0.0	0.6194348252682079	0.0
7	0.0	0.0	0.0	0.7631142005164774	0.0
8	0.0	0.0	0.0	1.0354135084736256	0.0
9	0.0	0.0	0.0	1.1256164959289563	0.0
10	7.683389050709599E-5	0.0	0.0	1.2804367853007548	0.0
11	7.683389050709599E-5	0.0	0.0	1.4848149340496302	0.0
12	7.683389050709599E-5	0.0	0.0	1.6515444764500284	0.0
13	7.683389050709599E-5	0.0	0.0	1.7393656132996391	0.0
14	7.683389050709599E-5	0.0	0.0	1.7664111427581368	0.0
15	7.683389050709599E-5	0.0	0.0	1.8283392585068563	0.0
16	7.683389050709599E-5	0.0	0.0	1.9157762259039315	0.0
17	7.683389050709599E-5	0.0	0.0	2.017811632497355	0.0
18	7.683389050709599E-5	0.0	0.0	2.140899525089723	0.0
19	7.683389050709599E-5	0.0	0.0	2.215812568334141	0.0
20	1.5366778101419198E-4	0.0	0.0	2.3040178746362874	0.0
21	1.5366778101419198E-4	0.0	0.0	2.426106926652063	0.0
22	1.5366778101419198E-4	0.0	0.0	2.559721062243903	0.0
23	1.5366778101419198E-4	0.0	0.0	2.7056286203168782	0.0
24	1.5366778101419198E-4	0.0	0.0	2.8111983858736282	0.0
25	1.5366778101419198E-4	0.0	0.0	2.9289847400210065	0.0
26	1.5366778101419198E-4	0.0	0.0	3.0285614621182027	0.0
27	1.5366778101419198E-4	0.0	0.0	3.122375642427367	0.0
28	1.5366778101419198E-4	0.0	0.0	3.2188790089042794	0.0
29	1.5366778101419198E-4	0.0	0.0	3.3211449171692244	0.0
30	1.5366778101419198E-4	0.0	0.0	3.4573714050383058	0.0
31	1.5366778101419198E-4	0.0	0.0	3.577846945353432	0.0
32	1.5366778101419198E-4	0.0	0.0	3.695479631719796	0.0
33	1.5366778101419198E-4	0.0	0.0	3.798206543327783	0.0
34	1.5366778101419198E-4	0.0	0.0	3.9279789843942683	0.0
35	1.5366778101419198E-4	0.0	0.0	4.063206631686757	0.0
36	1.5366778101419198E-4	0.0	0.0	4.173616932345454	0.0
37	1.5366778101419198E-4	0.0	0.0	4.291787455945368	0.0
38	1.5366778101419198E-4	0.0	0.0	4.426784601566336	0.0
39	1.5366778101419198E-4	0.0	0.0	4.644224511701418	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAC	25	3.8910905E-5	45.000004	8
CGCGATT	30	2.1657233E-6	45.000004	23
GTTCGCG	30	2.1657233E-6	45.000004	1
CGGACCG	25	3.8910905E-5	45.000004	6
GCGGTAT	20	7.033631E-4	45.0	36
GTTCGAC	20	7.033631E-4	45.0	38
TCGACTC	20	7.033631E-4	45.0	24
CGGTCTA	180	0.0	42.5	31
CGTTTTT	4535	0.0	42.32084	1
TACGTAG	80	0.0	42.1875	1
GTATGCG	65	0.0	41.53846	1
TAGTACG	65	0.0	41.53846	1
TTAGGCG	55	6.184564E-11	40.909092	1
TTTAGCG	160	0.0	40.78125	1
TACGCGG	50	1.0822987E-9	40.500004	2
ACGGTCT	190	0.0	40.26316	30
CACGACG	185	0.0	40.135136	26
GGCGTAG	90	0.0	40.0	1
TACGGGA	265	0.0	39.905663	4
TAAACGG	120	0.0	39.375004	2
>>END_MODULE