FastQCFastQC Report
Thu 26 May 2016
SRR1547698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547698_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1079721
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49690.46021148055840355No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44660.4136253717395512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA302.1653796E-645.00000416
CTATGCG750.045.0000041
GATCGCT302.1653796E-645.0000049
GTCCTCG302.1653796E-645.00000437
CCGTCTA302.1653796E-645.00000440
ATCCGGC302.1653796E-645.00000434
GCCGATC302.1653796E-645.0000049
GTTCGGC302.1653796E-645.00000418
AACGCCG302.1653796E-645.00000413
CGGGCAA750.045.0000046
ACGCTAG302.1653796E-645.0000041
ACGTCGT302.1653796E-645.00000410
CTCGACT302.1653796E-645.00000423
TTCGGCG302.1653796E-645.0000041
ACCGTGC302.1653796E-645.00000433
GCGGAGT302.1653796E-645.00000416
TATACGC302.1653796E-645.00000417
TTACCGG302.1653796E-645.0000042
GCTCGAC302.1653796E-645.00000422
TCGACTA302.1653796E-645.00000424
Produced by FastQC (version 0.11.5)