##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547698_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1079721
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623714830034796	31.0	31.0	33.0	30.0	34.0
2	31.997853149100553	33.0	31.0	34.0	30.0	34.0
3	32.040507686707954	33.0	31.0	34.0	30.0	34.0
4	35.739419720464824	37.0	35.0	37.0	33.0	37.0
5	35.662427608613704	37.0	35.0	37.0	33.0	37.0
6	35.7163971062895	37.0	35.0	37.0	33.0	37.0
7	36.09457813638894	37.0	35.0	37.0	35.0	37.0
8	36.00688418582208	37.0	35.0	37.0	35.0	37.0
9	37.890628227106816	39.0	38.0	39.0	35.0	39.0
10	37.47655366525241	39.0	37.0	39.0	35.0	39.0
11	37.07425621989384	39.0	37.0	39.0	33.0	39.0
12	35.652185147829854	35.0	35.0	39.0	32.0	39.0
13	35.06791291453996	35.0	35.0	39.0	31.0	39.0
14	35.817854797674585	36.0	35.0	40.0	31.0	41.0
15	36.252473555668544	36.0	35.0	40.0	32.0	41.0
16	36.50570934528457	36.0	35.0	40.0	32.0	41.0
17	36.4746244631715	36.0	35.0	40.0	32.0	41.0
18	36.43543100486144	36.0	35.0	40.0	32.0	41.0
19	36.354986149199654	36.0	35.0	40.0	32.0	41.0
20	36.167515497058965	36.0	35.0	40.0	32.0	41.0
21	35.936384491919675	35.0	35.0	40.0	31.0	41.0
22	35.8687049710064	35.0	35.0	40.0	31.0	41.0
23	35.8779758845109	35.0	35.0	40.0	31.0	41.0
24	35.86300535045628	35.0	35.0	40.0	31.0	41.0
25	35.79102379225744	35.0	35.0	40.0	31.0	41.0
26	35.74367544949112	35.0	35.0	40.0	31.0	41.0
27	35.63088612706431	35.0	34.0	40.0	31.0	41.0
28	35.74708095887734	36.0	35.0	40.0	31.0	41.0
29	35.759243360090245	36.0	35.0	40.0	31.0	41.0
30	35.788239739710534	36.0	35.0	40.0	31.0	41.0
31	35.52741680489682	35.0	35.0	40.0	31.0	41.0
32	35.264992530477784	35.0	34.0	40.0	30.0	41.0
33	35.132131356155895	35.0	34.0	40.0	30.0	41.0
34	35.008223420679975	35.0	34.0	40.0	30.0	41.0
35	34.901045733110685	35.0	34.0	40.0	29.0	41.0
36	34.733879400326565	35.0	34.0	40.0	27.0	41.0
37	34.44922901379152	35.0	34.0	40.0	27.0	41.0
38	34.62025652923302	35.0	34.0	40.0	27.0	41.0
39	34.629124560881934	35.0	34.0	40.0	27.0	41.0
40	34.48425287643752	35.0	34.0	40.0	27.0	41.0
41	34.499039103620284	35.0	34.0	40.0	27.0	41.0
42	34.51328259800449	35.0	34.0	40.0	27.0	41.0
43	34.365811167885035	35.0	34.0	40.0	27.0	41.0
44	34.27092369232422	35.0	34.0	40.0	27.0	41.0
45	34.257376674159346	35.0	34.0	40.0	27.0	41.0
46	34.080388359585484	35.0	34.0	39.0	26.0	41.0
47	33.96384621582798	35.0	33.0	39.0	25.0	41.0
48	33.8860094413279	35.0	34.0	39.0	24.0	41.0
49	34.06413508674926	35.0	34.0	39.0	26.0	41.0
50	33.86888001622641	35.0	34.0	39.0	24.0	41.0
51	33.21347737054295	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	2.0
11	0.0
12	2.0
13	3.0
14	8.0
15	20.0
16	71.0
17	188.0
18	447.0
19	863.0
20	1574.0
21	2503.0
22	3965.0
23	5986.0
24	8689.0
25	12676.0
26	16793.0
27	19528.0
28	20980.0
29	22810.0
30	26735.0
31	32688.0
32	41983.0
33	57640.0
34	121787.0
35	254653.0
36	51663.0
37	63201.0
38	102441.0
39	209769.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.560302152130042	25.39313396701555	28.300181250526755	15.746382630327648
2	29.556153858265237	25.640049605407324	31.099145056917482	13.70465147940996
3	27.197581597468236	25.584664927328447	32.542388265116635	14.67536521008668
4	24.965801350534072	27.544986158461306	32.550260669191395	14.938951821813228
5	22.650481003888967	31.710877161785312	31.885644532244907	13.752997302080816
6	20.056477552997485	39.540214555426815	29.06593462570423	11.337373265871461
7	87.64190008344748	4.2824025836304	6.631898425611801	1.4437989073103146
8	90.10411022847569	2.649480745488881	5.771490968500196	1.4749180575352336
9	88.78247250910188	2.9931806457408903	6.6674631687259955	1.5568836764312262
10	61.4678236322161	17.575558871226917	11.49259855092195	9.464018945635031
11	56.10569767560324	19.05242187565121	16.154543627474137	8.68733682127142
12	51.11950216768961	20.433982482511688	18.257123831063765	10.189391518734931
13	20.49881404548027	48.90068823334917	19.796595602012	10.803902119158561
14	11.202894080970918	51.57313787543264	25.693767186152723	11.53020085744373
15	9.494952862822895	22.98093674199168	57.42400120031008	10.100109194875342
16	11.080825509552932	17.45052657121608	55.82034618202295	15.648301737208037
17	12.363471674627057	17.23658241341976	27.8320973658936	42.56784854605959
18	19.87059620031471	22.755322902861018	38.290076788355506	19.08400410846876
19	30.526682355904907	23.49301347292495	26.267341285387612	19.71296288578253
20	33.41001981067331	22.86423992864823	25.03609728809572	18.68964297258273
21	19.889489970094125	29.11659586133825	30.403595002783124	20.590319165784493
22	20.616251790971926	23.30240867779732	26.67652106423789	29.40481846699286
23	15.501319322306411	30.54955863598096	26.490269245481006	27.45885279623162
24	17.614272575971015	22.84645755709114	43.656000022227964	15.883269844709883
25	11.94864228814666	24.249319963212717	41.83914177829273	21.962895970347894
26	12.743106784067365	38.744360811728214	27.898873875751235	20.613658528453183
27	14.362599227022535	40.11953087881036	30.431657807896666	15.086212086270434
28	10.178277536511747	30.99458100750101	44.56623516630685	14.260906289680388
29	10.996174011619669	23.98656690015291	44.41795612014585	20.59930296808157
30	15.289227494880622	33.50087661534785	34.63200215611255	16.577893733658975
31	31.203801722852475	27.18378173620778	24.535227155904163	17.07718938503558
32	31.831741718462453	27.383370333632485	26.34652840872781	14.43835953917725
33	29.14734454548907	29.84215366747521	23.584240743673597	17.42626104336213
34	16.8117504429385	30.69811553169754	26.725793052094016	25.764340973269945
35	16.151672515399813	27.695951083659576	32.90544501774069	23.24693138319992
36	32.96925779900548	21.651426618543123	29.339338588394593	16.039976994056797
37	17.24899302690232	33.75001505018426	32.81421774699205	16.18677417592137
38	17.212224269047283	35.633186721384504	23.670559338940336	23.484029670627876
39	17.436541476918574	33.494763925125106	30.766559138888656	18.302135459067667
40	23.932756702888987	26.867866791513734	24.626361810134284	24.573014695462994
41	14.833646840248546	22.94000024080295	29.076029826223625	33.15032309272488
42	22.110156234805103	23.141163319042604	25.154646431809695	29.594034014342597
43	22.300668413414208	24.051120613565914	26.3684785236186	27.27973244940128
44	16.813139690716397	32.70706043505683	29.327298440986144	21.152501433240626
45	13.917669472021013	44.44444444444444	21.21853701095005	20.41934907258449
46	23.347790771875328	35.75256941376522	23.62063903545453	17.279000778904923
47	20.637460973714504	29.265337990091883	25.47019091042964	24.62701012576397
48	22.955004116804247	24.1013187666073	32.09894037441154	20.844736742176913
49	21.028673147970633	21.361999998147667	34.402035340611135	23.207291513270558
50	18.559887230127043	35.18881266549414	27.465150719491426	18.786149384887395
51	16.167139473993743	37.087636528325376	24.016759885192563	22.728464112488318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1303.0
1	1310.0
2	1317.0
3	4323.5
4	7330.0
5	5517.5
6	3705.0
7	4191.0
8	4677.0
9	5404.0
10	6131.0
11	6432.5
12	6734.0
13	6246.0
14	5758.0
15	5589.0
16	5420.0
17	4862.0
18	4304.0
19	4087.0
20	3870.0
21	3816.5
22	3763.0
23	3526.0
24	3289.0
25	4000.0
26	5543.5
27	6376.0
28	8393.5
29	10411.0
30	12673.5
31	14936.0
32	17647.0
33	20358.0
34	21755.0
35	23152.0
36	25736.5
37	28321.0
38	35086.0
39	41851.0
40	67297.5
41	92744.0
42	119493.0
43	146242.0
44	151116.5
45	155991.0
46	140223.0
47	124455.0
48	110245.0
49	96035.0
50	85753.5
51	75472.0
52	64912.5
53	54353.0
54	48011.0
55	41669.0
56	35573.0
57	29477.0
58	26095.5
59	22714.0
60	17798.5
61	12883.0
62	11132.0
63	9381.0
64	7248.5
65	5116.0
66	4029.0
67	2942.0
68	2018.5
69	1095.0
70	969.0
71	843.0
72	558.0
73	273.0
74	216.0
75	118.5
76	78.0
77	51.0
78	24.0
79	31.5
80	39.0
81	22.0
82	5.0
83	4.5
84	4.0
85	2.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	3.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1079721.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.428190493919757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.80111034930194	14.430735242757049
2	7.053523467921967	2.4586030130455505
3	2.055260236182263	1.0745840073228898
4	0.9313662763901399	0.649281155381603
5	0.5572470790023888	0.4855904122516992
6	0.404050560491021	0.4225122082447537
7	0.2762153148109363	0.33697531867041086
8	0.23371427782539037	0.3258573564071831
9	0.1766346977566698	0.2770580844305264
>10	2.2016420816038544	9.601188212275114
>50	1.3964599950270078	18.364759663861307
>100	1.9063551579621554	50.11305531754391
>500	0.004815379293196576	0.4858229345691304
>1k	0.001605126431065525	0.9739770732388804
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4969	0.46021148055840355	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4466	0.4136253717395512	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07714955993261222	0.0
2	0.0	0.0	0.0	0.2749784435053129	0.0
3	0.0	0.0	0.0	0.3663909472910131	0.0
4	0.0	0.0	0.0	0.5504199696032587	0.0
5	0.0	0.0	0.0	0.9041224538561351	0.0
6	0.0	0.0	0.0	1.2439324603300297	0.0
7	0.0	0.0	0.0	1.439816397013673	0.0
8	0.0	0.0	0.0	1.8502001906047951	0.0
9	0.0	0.0	0.0	1.94818846720588	0.0
10	0.0	0.0	0.0	2.1249007845545282	0.0
11	0.0	0.0	0.0	2.40099062628216	0.0
12	0.0	0.0	0.0	2.5970597960028563	0.0
13	0.0	0.0	0.0	2.6782844827506365	0.0
14	0.0	0.0	0.0	2.708292234753237	0.0
15	0.0	0.0	0.0	2.7595091694984166	0.0
16	0.0	0.0	0.0	2.873520103804594	0.0
17	0.0	0.0	0.0	2.9866974894440323	0.0
18	0.0	0.0	0.0	3.1542407714585528	0.0
19	0.0	0.0	0.0	3.2333352782802223	0.0
20	0.0	0.0	0.0	3.3212283543619137	0.0
21	0.0	0.0	0.0	3.465154424152165	0.0
22	0.0	0.0	0.0	3.5961141813487	0.0
23	0.0	0.0	0.0	3.7696775370674462	0.0
24	0.0	0.0	0.0	3.8846146365588887	0.0
25	0.0	0.0	0.0	3.979268718493018	0.0
26	0.0	0.0	0.0	4.078275776797895	0.0
27	0.0	0.0	0.0	4.161074944360626	0.0
28	0.0	0.0	0.0	4.245911675330942	0.0
29	0.0	0.0	0.0	4.3433442528208674	0.0
30	0.0	0.0	0.0	4.475600641276774	0.0
31	0.0	0.0	0.0	4.604708068102778	0.0
32	0.0	0.0	0.0	4.701399713444491	0.0
33	9.261651852654528E-5	0.0	0.0	4.8051302141942225	0.0
34	9.261651852654528E-5	0.0	0.0	4.910713045314484	0.0
35	9.261651852654528E-5	0.0	0.0	5.062141053105385	0.0
36	9.261651852654528E-5	0.0	0.0	5.17300302578166	0.0
37	9.261651852654528E-5	0.0	0.0	5.2883105913472095	0.0
38	9.261651852654528E-5	0.0	0.0	5.401395360468121	0.0
39	9.261651852654528E-5	0.0	0.0	5.51105331840355	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	30	2.1653796E-6	45.000004	16
CTATGCG	75	0.0	45.000004	1
GATCGCT	30	2.1653796E-6	45.000004	9
GTCCTCG	30	2.1653796E-6	45.000004	37
CCGTCTA	30	2.1653796E-6	45.000004	40
ATCCGGC	30	2.1653796E-6	45.000004	34
GCCGATC	30	2.1653796E-6	45.000004	9
GTTCGGC	30	2.1653796E-6	45.000004	18
AACGCCG	30	2.1653796E-6	45.000004	13
CGGGCAA	75	0.0	45.000004	6
ACGCTAG	30	2.1653796E-6	45.000004	1
ACGTCGT	30	2.1653796E-6	45.000004	10
CTCGACT	30	2.1653796E-6	45.000004	23
TTCGGCG	30	2.1653796E-6	45.000004	1
ACCGTGC	30	2.1653796E-6	45.000004	33
GCGGAGT	30	2.1653796E-6	45.000004	16
TATACGC	30	2.1653796E-6	45.000004	17
TTACCGG	30	2.1653796E-6	45.000004	2
GCTCGAC	30	2.1653796E-6	45.000004	22
TCGACTA	30	2.1653796E-6	45.000004	24
>>END_MODULE