FastQCFastQC Report
Thu 26 May 2016
SRR1547697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547697_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1825288
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102720.5627605068350858No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35370.19377763947388027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG650.045.0000041
ACGTTGT351.2124474E-745.029
ATTACGA253.891711E-545.011
CCAATCG700.045.024
GCGCCTA207.0343795E-445.019
TATATCG253.891711E-545.028
CGTTCGA351.2124474E-745.038
ATCGTAG406.8193913E-945.01
AATGCGT453.8562575E-1045.026
ATATCGG351.2124474E-745.02
TACGATA253.891711E-545.013
TCGTCCC253.891711E-545.038
CTCGCTA351.2124474E-745.041
GCGATAC351.2124474E-745.09
CGTCGCG253.891711E-545.039
GCGTGCC207.0343795E-445.038
CGCCGAT253.891711E-545.030
TAATGCG950.045.01
CCGTTCG253.891711E-545.041
TATCCGT207.0343795E-445.034