FastQCFastQC Report
Thu 26 May 2016
SRR1547696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547696_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1470598
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.48368078835956524No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25110.17074686624080818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC207.033929E-445.00000429
ACACGTA207.033929E-445.00000439
GCGAATA207.033929E-445.00000441
CACGCTT207.033929E-445.00000442
TATTACG207.033929E-445.0000041
ATACGTC207.033929E-445.00000426
TCGACCA207.033929E-445.00000420
TGCGTCG406.8175723E-945.0000041
TCGCAAG502.1827873E-1145.01
CGCGAGC253.891339E-545.037
TACGCTA253.891339E-545.035
CTTATCG351.2122291E-745.01
CGCTAAG253.891339E-545.037
GAACGTA453.8562575E-1045.09
CGTAAAG253.891339E-545.035
TTACGCG253.891339E-545.01
CGCGTCA453.8562575E-1045.039
TAGTCGA253.891339E-545.027
CGAGATC302.165918E-644.99999620
CGTTTTT45850.042.9880071