##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547696_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1470598
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.687646114029803	31.0	31.0	33.0	30.0	34.0
2	32.06098267507504	33.0	31.0	34.0	30.0	34.0
3	32.12035580083748	33.0	31.0	34.0	30.0	34.0
4	35.81394643539567	37.0	35.0	37.0	35.0	37.0
5	35.740807481038324	37.0	35.0	37.0	33.0	37.0
6	35.79445436482302	37.0	35.0	37.0	35.0	37.0
7	36.146870184781974	37.0	35.0	37.0	35.0	37.0
8	36.05570523011727	37.0	35.0	37.0	35.0	37.0
9	37.88535888121703	39.0	38.0	39.0	35.0	39.0
10	37.51949751053653	39.0	37.0	39.0	35.0	39.0
11	37.09663959831307	39.0	37.0	39.0	33.0	39.0
12	36.20907141176583	37.0	35.0	39.0	33.0	39.0
13	35.89095796403912	37.0	35.0	39.0	32.0	39.0
14	36.80807875435707	38.0	35.0	41.0	32.0	41.0
15	37.07455878492967	39.0	35.0	41.0	33.0	41.0
16	37.22825952435676	38.0	35.0	41.0	33.0	41.0
17	37.145307555157835	38.0	35.0	41.0	33.0	41.0
18	37.08542987274564	38.0	35.0	41.0	33.0	41.0
19	37.026519143912886	38.0	35.0	41.0	32.0	41.0
20	36.88562543944708	38.0	35.0	41.0	32.0	41.0
21	36.73620255161506	38.0	35.0	41.0	32.0	41.0
22	36.68403873798278	38.0	35.0	41.0	32.0	41.0
23	36.658987704321646	38.0	35.0	41.0	32.0	41.0
24	36.64332196834213	38.0	35.0	41.0	32.0	41.0
25	36.582471892386636	38.0	35.0	40.0	32.0	41.0
26	36.545094580571984	38.0	35.0	40.0	32.0	41.0
27	36.45326731030506	38.0	35.0	40.0	32.0	41.0
28	36.479587895536376	38.0	35.0	40.0	32.0	41.0
29	36.457650561200275	37.0	35.0	40.0	32.0	41.0
30	36.47508360544486	37.0	35.0	40.0	32.0	41.0
31	36.31329635971217	37.0	35.0	40.0	31.0	41.0
32	36.09378769724969	37.0	35.0	40.0	31.0	41.0
33	36.01008025306712	38.0	35.0	40.0	30.0	41.0
34	35.828471138951635	37.0	35.0	40.0	30.0	41.0
35	35.72588566011922	37.0	35.0	40.0	30.0	41.0
36	35.637040850048756	37.0	35.0	40.0	30.0	41.0
37	35.368913190416414	37.0	34.0	40.0	29.0	41.0
38	35.489624628892464	37.0	35.0	40.0	29.0	41.0
39	35.442675700633345	37.0	35.0	40.0	29.0	41.0
40	35.301596357400186	37.0	34.0	40.0	29.0	41.0
41	35.287483731108026	37.0	34.0	40.0	29.0	41.0
42	35.29433060564478	37.0	34.0	40.0	29.0	41.0
43	35.20079722670641	36.0	34.0	40.0	29.0	41.0
44	35.064240533442856	36.0	34.0	40.0	28.0	41.0
45	35.045901055216994	36.0	34.0	40.0	28.0	41.0
46	34.879394640819584	36.0	34.0	40.0	27.0	41.0
47	34.773821941822305	36.0	34.0	40.0	27.0	41.0
48	34.67466228024246	35.0	34.0	40.0	27.0	41.0
49	34.764118406253786	36.0	34.0	40.0	27.0	41.0
50	34.60474650448321	35.0	34.0	40.0	27.0	41.0
51	33.99337004402291	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	10.0
14	8.0
15	38.0
16	75.0
17	203.0
18	487.0
19	918.0
20	1714.0
21	2779.0
22	4350.0
23	6547.0
24	9579.0
25	14310.0
26	19213.0
27	22649.0
28	24199.0
29	26954.0
30	31537.0
31	39364.0
32	50551.0
33	68375.0
34	135552.0
35	257472.0
36	83712.0
37	109179.0
38	182191.0
39	378536.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.099797497344618	23.52621178595374	30.9746783281359	14.399312388565741
2	29.58361156481921	24.870358860817166	32.19540622250268	13.350623351860943
3	28.459783027040704	24.780123459980224	33.584161001170955	13.175932511808123
4	25.959847626611758	26.407148656532918	32.61843141361541	15.014572303239907
5	24.655412288062408	30.690372215928484	30.700164150910037	13.954051345099069
6	22.087545338698952	40.40077573884909	26.87321756183539	10.638461360616565
7	88.21350226234497	3.6407638253281998	6.6210480362410395	1.5246858760857829
8	90.09504976886954	2.7140659786019024	5.7268539736896145	1.4640302788389485
9	86.94537868268554	4.114652678706213	6.713391423080951	2.2265772155272887
10	55.4705636754572	25.491602735757834	11.155189929538869	7.882643659246103
11	43.453615467993295	19.801468518249038	22.858048222559802	13.886867791197865
12	40.8255009186739	20.5739433890159	24.134399747585675	14.466155944724527
13	23.23524171799499	38.089335086815026	24.055860269087816	14.61956292610217
14	14.362252634642505	41.13619085569272	28.186288843042085	16.31526766662269
15	14.238017459564068	25.497518696475858	45.42070640650946	14.843757437450616
16	17.392176515947934	21.429241709835047	44.621371714091815	16.5572100601252
17	17.969152684826174	21.22068709463769	28.834324540085056	31.97583568045108
18	21.911154509934054	24.135555739909886	34.20445288243286	19.7488368677232
19	27.588776810521974	25.96236360990563	27.22293923968345	19.225920339888944
20	30.085584231720702	24.94311837769397	27.04586841543372	17.925428975151604
21	22.97072347439613	27.76686762799895	30.33208259497157	18.93032630263335
22	22.61821381506027	21.930466381703226	29.442036504877606	26.0092832983589
23	19.138404920991327	28.034785849021965	28.074157587593618	24.752651642393094
24	19.775764688922465	23.98928871112296	38.90920564287453	17.325740957080047
25	17.11671034504331	25.986367450520127	35.60762356537953	21.289298639057037
26	16.339339506785674	35.240426003571336	28.165480981206287	20.254753508436703
27	16.614669678593334	34.93497203178571	31.58320628751025	16.867152002110704
28	14.545919415095085	29.853705771393678	38.86201395622733	16.738360857283908
29	15.035108166881772	25.073609511232846	38.69990303264386	21.191379289241517
30	18.064284053153887	30.07334431299376	34.23865665531981	17.623714978532544
31	28.31861596439	26.03179114890677	27.00119271208039	18.64840017462284
32	28.159973017779162	27.307054681156917	28.156233042612598	16.376739258451323
33	27.592312787043095	27.917078630597892	26.638483120472078	17.852125461886935
34	19.77175271556197	28.449923092510666	29.136922530834397	22.64140166109297
35	18.623988336717446	27.848467086178548	31.558862449153334	21.96868212795067
36	29.257213732100816	23.57245147892218	29.50235210438203	17.667982684594975
37	20.007303151507074	31.04743784501271	30.980730287950887	17.96452871552933
38	19.477858667018452	32.420756726175334	25.995887387307747	22.105497219498464
39	19.543002234465163	30.773603663271675	29.813858035982644	19.869536066280517
40	23.002683262183137	26.398580713424064	27.440061797989664	23.158674226403136
41	18.0546281172693	24.204711280717095	30.00548076360773	27.73517983840587
42	22.265160159336542	24.406737939260083	27.44645375554706	25.88164814585631
43	23.0104352107102	25.50465864906657	27.11617994856514	24.368726191658087
44	19.730748987826722	30.406882098302866	28.75666905571747	21.105699858152942
45	18.106035775922447	37.11687354395966	24.34111837497399	20.435972305143892
46	22.91401185096131	31.666097737111027	26.767750262138257	18.652140149789407
47	21.494929273669623	28.992083492565605	27.291142786811896	22.221844446952872
48	23.519003833814544	25.271352198221404	31.057229779994262	20.152414187969793
49	22.180364722378243	23.44046435531668	32.013575429859145	22.36559549244593
50	20.10087053021968	30.871591012635673	29.284073553751604	19.74346490339304
51	19.623309701223583	32.63475130525133	25.899396028010376	21.842542965514706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1194.0
1	1727.5
2	2261.0
3	7222.0
4	12183.0
5	8793.0
6	5403.0
7	5765.5
8	6128.0
9	6494.0
10	6860.0
11	6935.0
12	7010.0
13	6806.5
14	6603.0
15	6044.0
16	5485.0
17	5204.5
18	4924.0
19	4892.5
20	4861.0
21	5318.0
22	5775.0
23	6897.0
24	8019.0
25	7656.0
26	9967.5
27	12642.0
28	16971.5
29	21301.0
30	23512.0
31	25723.0
32	28928.0
33	32133.0
34	34656.0
35	37179.0
36	43697.5
37	50216.0
38	58753.0
39	67290.0
40	87485.0
41	107680.0
42	129164.5
43	150649.0
44	151323.0
45	151997.0
46	149694.5
47	147392.0
48	137248.0
49	127104.0
50	115762.5
51	104421.0
52	96586.5
53	88752.0
54	80608.0
55	72464.0
56	63548.5
57	54633.0
58	49507.0
59	44381.0
60	38677.5
61	32974.0
62	28977.5
63	24981.0
64	19974.5
65	14968.0
66	11448.0
67	7928.0
68	6069.5
69	4211.0
70	3265.0
71	2319.0
72	1892.0
73	1465.0
74	1138.5
75	620.0
76	428.0
77	391.5
78	355.0
79	242.0
80	129.0
81	74.5
82	20.0
83	21.5
84	23.0
85	16.5
86	10.0
87	9.5
88	9.0
89	8.5
90	8.0
91	4.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1470598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.657517878382937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.33550851763259	14.53840713675848
2	6.512737078950538	2.299975428175532
3	1.8922164143394367	1.0023553549790478
4	0.9199797558281396	0.6497823594514297
5	0.541451287700659	0.4780342896423925
6	0.39685909774181544	0.4204527968145016
7	0.2742824202721276	0.33902027177768645
8	0.25229558404305763	0.3563931028701883
9	0.21056971669948307	0.33463246835404376
>10	3.120414562209315	14.244622688338351
>50	1.9930565495544637	26.172556644515826
>100	1.545588210528377	37.970853574258385
>500	0.004265296115356785	0.5339801057117839
>1k	3.877541923051623E-4	0.17192256000029355
>5k	3.877541923051623E-4	0.487011218352086
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7113	0.48368078835956524	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2511	0.17074686624080818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.046443691613887686	0.0
2	0.0	0.0	0.0	0.16088693171077345	0.0
3	0.0	0.0	0.0	0.211274597136675	0.0
4	0.0	0.0	0.0	0.29063007021633375	0.0
5	0.0	0.0	0.0	0.48191280009900733	0.0
6	0.0	0.0	0.0	0.6205638794558405	0.0
7	0.0	0.0	0.0	0.7180072324319766	0.0
8	0.0	0.0	0.0	0.9332938029291485	0.0
9	0.0	0.0	0.0	1.0025173432848407	0.0
10	0.0	0.0	0.0	1.1969280523977321	0.0
11	0.0	0.0	0.0	1.3946707393862905	0.0
12	0.0	0.0	0.0	1.5777935234510043	0.0
13	0.0	0.0	0.0	1.6412371021856416	0.0
14	0.0	0.0	0.0	1.6678929251909766	0.0
15	0.0	0.0	0.0	1.7131806244806536	0.0
16	0.0	0.0	0.0	1.805796009514497	0.0
17	0.0	0.0	0.0	1.9194912545780696	0.0
18	0.0	0.0	0.0	2.0659622820104473	0.0
19	0.0	0.0	0.0	2.1642896291168627	0.0
20	0.0	0.0	0.0	2.250581056141787	0.0
21	0.0	0.0	0.0	2.3733882407020817	0.0
22	0.0	0.0	0.0	2.498643409007764	0.0
23	0.0	0.0	0.0	2.63668249242825	0.0
24	0.0	0.0	0.0	2.7416737952859993	0.0
25	0.0	0.0	0.0	2.8329291893501827	0.0
26	0.0	0.0	0.0	2.9240485843174002	0.0
27	0.0	0.0	0.0	3.01000001359991	0.0
28	0.0	0.0	0.0	3.098943423015671	0.0
29	0.0	0.0	0.0	3.1996507543189914	0.0
30	0.0	0.0	0.0	3.3335418652820143	0.0
31	0.0	0.0	0.0	3.455737053905962	0.0
32	0.0	0.0	0.0	3.558008374824391	0.0
33	0.0	0.0	0.0	3.662931678133657	0.0
34	0.0	0.0	0.0	3.7676509827974742	0.0
35	0.0	0.0	0.0	3.921873958756914	0.0
36	0.0	0.0	0.0	4.042097160474855	0.0
37	0.0	0.0	0.0	4.1690523174926115	0.0
38	0.0	0.0	0.0	4.2970954672860975	0.0
39	0.0	0.0	0.0	4.461518375517986	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	20	7.033929E-4	45.000004	29
ACACGTA	20	7.033929E-4	45.000004	39
GCGAATA	20	7.033929E-4	45.000004	41
CACGCTT	20	7.033929E-4	45.000004	42
TATTACG	20	7.033929E-4	45.000004	1
ATACGTC	20	7.033929E-4	45.000004	26
TCGACCA	20	7.033929E-4	45.000004	20
TGCGTCG	40	6.8175723E-9	45.000004	1
TCGCAAG	50	2.1827873E-11	45.0	1
CGCGAGC	25	3.891339E-5	45.0	37
TACGCTA	25	3.891339E-5	45.0	35
CTTATCG	35	1.2122291E-7	45.0	1
CGCTAAG	25	3.891339E-5	45.0	37
GAACGTA	45	3.8562575E-10	45.0	9
CGTAAAG	25	3.891339E-5	45.0	35
TTACGCG	25	3.891339E-5	45.0	1
CGCGTCA	45	3.8562575E-10	45.0	39
TAGTCGA	25	3.891339E-5	45.0	27
CGAGATC	30	2.165918E-6	44.999996	20
CGTTTTT	4585	0.0	42.988007	1
>>END_MODULE