FastQCFastQC Report
Thu 26 May 2016
SRR1547695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547695_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2219929
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97550.43942846820776704No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39120.17622185214031622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT351.2126111E-745.00000411
TCGTTAG207.03471E-445.01
TAGACGT253.891984E-545.027
CGTGATA551.8189894E-1245.039
CGTTGAC406.8193913E-945.019
CCGCTAT207.03471E-445.038
CGTTACA207.03471E-445.013
CTAACCG253.891984E-545.023
CTCGATA406.8193913E-945.024
CGTAAGA253.891984E-545.012
TATAGCG551.8189894E-1245.01
CAGTCGA207.03471E-445.01
TGAATCG207.03471E-445.013
GTAACGA207.03471E-445.020
AATCGAT207.03471E-445.020
AGTCGAC253.891984E-545.042
TAACGCG302.16642E-644.99999618
CACGTAT302.16642E-644.99999636
CGTTTTT71900.042.7155761
CTTGACG1650.042.2727281